The nature of cell division forces in epithelial monolayers
<jats:p>Epithelial cells undergo striking morphological changes during division to ensure proper segregation of genetic and cytoplasmic materials. These morphological changes occur despite dividing cells being mechanically restricted by neighboring cells, indicating the need for extracellular...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Rockefeller University Press
2021
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| Online Access: | https://hdl.handle.net/1721.1/135609 |
| _version_ | 1826205337401688064 |
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| author | Gupta, Vivek K Nam, Sungmin Yim, Donghyun Camuglia, Jaclyn Martin, Judy Lisette Sanders, Erin Nicole O’Brien, Lucy Erin Martin, Adam C Kim, Taeyoon Chaudhuri, Ovijit |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Gupta, Vivek K Nam, Sungmin Yim, Donghyun Camuglia, Jaclyn Martin, Judy Lisette Sanders, Erin Nicole O’Brien, Lucy Erin Martin, Adam C Kim, Taeyoon Chaudhuri, Ovijit |
| author_sort | Gupta, Vivek K |
| collection | MIT |
| description | <jats:p>Epithelial cells undergo striking morphological changes during division to ensure proper segregation of genetic and cytoplasmic materials. These morphological changes occur despite dividing cells being mechanically restricted by neighboring cells, indicating the need for extracellular force generation. Beyond driving cell division itself, forces associated with division have been implicated in tissue-scale processes, including development, tissue growth, migration, and epidermal stratification. While forces generated by mitotic rounding are well understood, forces generated after rounding remain unknown. Here, we identify two distinct stages of division force generation that follow rounding: (1) Protrusive forces along the division axis that drive division elongation, and (2) outward forces that facilitate postdivision spreading. Cytokinetic ring contraction of the dividing cell, but not activity of neighboring cells, generates extracellular forces that propel division elongation and contribute to chromosome segregation. Forces from division elongation are observed in epithelia across many model organisms. Thus, division elongation forces represent a universal mechanism that powers cell division in confining epithelia.</jats:p> |
| first_indexed | 2024-09-23T13:11:06Z |
| format | Article |
| id | mit-1721.1/135609 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T13:11:06Z |
| publishDate | 2021 |
| publisher | Rockefeller University Press |
| record_format | dspace |
| spelling | mit-1721.1/1356092023-12-19T21:03:11Z The nature of cell division forces in epithelial monolayers Gupta, Vivek K Nam, Sungmin Yim, Donghyun Camuglia, Jaclyn Martin, Judy Lisette Sanders, Erin Nicole O’Brien, Lucy Erin Martin, Adam C Kim, Taeyoon Chaudhuri, Ovijit Massachusetts Institute of Technology. Department of Biology <jats:p>Epithelial cells undergo striking morphological changes during division to ensure proper segregation of genetic and cytoplasmic materials. These morphological changes occur despite dividing cells being mechanically restricted by neighboring cells, indicating the need for extracellular force generation. Beyond driving cell division itself, forces associated with division have been implicated in tissue-scale processes, including development, tissue growth, migration, and epidermal stratification. While forces generated by mitotic rounding are well understood, forces generated after rounding remain unknown. Here, we identify two distinct stages of division force generation that follow rounding: (1) Protrusive forces along the division axis that drive division elongation, and (2) outward forces that facilitate postdivision spreading. Cytokinetic ring contraction of the dividing cell, but not activity of neighboring cells, generates extracellular forces that propel division elongation and contribute to chromosome segregation. Forces from division elongation are observed in epithelia across many model organisms. Thus, division elongation forces represent a universal mechanism that powers cell division in confining epithelia.</jats:p> 2021-10-27T20:24:14Z 2021-10-27T20:24:14Z 2021 2021-07-21T17:32:28Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/135609 en 10.1083/jcb.202011106 The Journal of Cell Biology Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Rockefeller University Press Rockefeller University Press |
| spellingShingle | Gupta, Vivek K Nam, Sungmin Yim, Donghyun Camuglia, Jaclyn Martin, Judy Lisette Sanders, Erin Nicole O’Brien, Lucy Erin Martin, Adam C Kim, Taeyoon Chaudhuri, Ovijit The nature of cell division forces in epithelial monolayers |
| title | The nature of cell division forces in epithelial monolayers |
| title_full | The nature of cell division forces in epithelial monolayers |
| title_fullStr | The nature of cell division forces in epithelial monolayers |
| title_full_unstemmed | The nature of cell division forces in epithelial monolayers |
| title_short | The nature of cell division forces in epithelial monolayers |
| title_sort | nature of cell division forces in epithelial monolayers |
| url | https://hdl.handle.net/1721.1/135609 |
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