Cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry
<jats:title>Abstract</jats:title><jats:p>Nanosensors have proven to be powerful tools to monitor single cells, achieving spatiotemporal precision even at molecular level. However, there has not been way of extending this approach to statistically relevant numbers of living cells. H...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer Science and Business Media LLC
2021
|
| Online Access: | https://hdl.handle.net/1721.1/135634 |
| _version_ | 1826201122287648768 |
|---|---|
| author | Cho, Soo-Yeon Gong, Xun Koman, Volodymyr B Kuehne, Matthias Moon, Sun Jin Son, Manki Lew, Tedrick Thomas Salim Gordiichuk, Pavlo Jin, Xiaojia Sikes, Hadley D Strano, Michael S |
| author_facet | Cho, Soo-Yeon Gong, Xun Koman, Volodymyr B Kuehne, Matthias Moon, Sun Jin Son, Manki Lew, Tedrick Thomas Salim Gordiichuk, Pavlo Jin, Xiaojia Sikes, Hadley D Strano, Michael S |
| author_sort | Cho, Soo-Yeon |
| collection | MIT |
| description | <jats:title>Abstract</jats:title><jats:p>Nanosensors have proven to be powerful tools to monitor single cells, achieving spatiotemporal precision even at molecular level. However, there has not been way of extending this approach to statistically relevant numbers of living cells. Herein, we design and fabricate nanosensor array in microfluidics that addresses this limitation, creating a Nanosensor Chemical Cytometry (NCC). nIR fluorescent carbon nanotube array is integrated along microfluidic channel through which flowing cells is guided. We can utilize the flowing cell itself as highly informative Gaussian lenses projecting nIR profiles and extract rich information. This unique biophotonic waveguide allows for quantified cross-correlation of biomolecular information with various physical properties and creates label-free chemical cytometer for cellular heterogeneity measurement. As an example, the NCC can profile the immune heterogeneities of human monocyte populations at attomolar sensitivity in completely non-destructive and real-time manner with rate of ~600 cells/hr, highest range demonstrated to date for state-of-the-art chemical cytometry.</jats:p> |
| first_indexed | 2024-09-23T11:46:34Z |
| format | Article |
| id | mit-1721.1/135634 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T11:46:34Z |
| publishDate | 2021 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1356342021-10-28T04:11:18Z Cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry Cho, Soo-Yeon Gong, Xun Koman, Volodymyr B Kuehne, Matthias Moon, Sun Jin Son, Manki Lew, Tedrick Thomas Salim Gordiichuk, Pavlo Jin, Xiaojia Sikes, Hadley D Strano, Michael S <jats:title>Abstract</jats:title><jats:p>Nanosensors have proven to be powerful tools to monitor single cells, achieving spatiotemporal precision even at molecular level. However, there has not been way of extending this approach to statistically relevant numbers of living cells. Herein, we design and fabricate nanosensor array in microfluidics that addresses this limitation, creating a Nanosensor Chemical Cytometry (NCC). nIR fluorescent carbon nanotube array is integrated along microfluidic channel through which flowing cells is guided. We can utilize the flowing cell itself as highly informative Gaussian lenses projecting nIR profiles and extract rich information. This unique biophotonic waveguide allows for quantified cross-correlation of biomolecular information with various physical properties and creates label-free chemical cytometer for cellular heterogeneity measurement. As an example, the NCC can profile the immune heterogeneities of human monocyte populations at attomolar sensitivity in completely non-destructive and real-time manner with rate of ~600 cells/hr, highest range demonstrated to date for state-of-the-art chemical cytometry.</jats:p> 2021-10-27T20:24:22Z 2021-10-27T20:24:22Z 2021 2021-06-17T17:27:46Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/135634 en 10.1038/s41467-021-23416-1 Nature Communications Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Springer Science and Business Media LLC Nature |
| spellingShingle | Cho, Soo-Yeon Gong, Xun Koman, Volodymyr B Kuehne, Matthias Moon, Sun Jin Son, Manki Lew, Tedrick Thomas Salim Gordiichuk, Pavlo Jin, Xiaojia Sikes, Hadley D Strano, Michael S Cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry |
| title | Cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry |
| title_full | Cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry |
| title_fullStr | Cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry |
| title_full_unstemmed | Cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry |
| title_short | Cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry |
| title_sort | cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry |
| url | https://hdl.handle.net/1721.1/135634 |
| work_keys_str_mv | AT chosooyeon cellularlensingandnearinfraredfluorescentnanosensorarraystoenablechemicaleffluxcytometry AT gongxun cellularlensingandnearinfraredfluorescentnanosensorarraystoenablechemicaleffluxcytometry AT komanvolodymyrb cellularlensingandnearinfraredfluorescentnanosensorarraystoenablechemicaleffluxcytometry AT kuehnematthias cellularlensingandnearinfraredfluorescentnanosensorarraystoenablechemicaleffluxcytometry AT moonsunjin cellularlensingandnearinfraredfluorescentnanosensorarraystoenablechemicaleffluxcytometry AT sonmanki cellularlensingandnearinfraredfluorescentnanosensorarraystoenablechemicaleffluxcytometry AT lewtedrickthomassalim cellularlensingandnearinfraredfluorescentnanosensorarraystoenablechemicaleffluxcytometry AT gordiichukpavlo cellularlensingandnearinfraredfluorescentnanosensorarraystoenablechemicaleffluxcytometry AT jinxiaojia cellularlensingandnearinfraredfluorescentnanosensorarraystoenablechemicaleffluxcytometry AT sikeshadleyd cellularlensingandnearinfraredfluorescentnanosensorarraystoenablechemicaleffluxcytometry AT stranomichaels cellularlensingandnearinfraredfluorescentnanosensorarraystoenablechemicaleffluxcytometry |