Cell diversity and network dynamics in photosensitive human brain organoids
© 2017 Macmillan Publishers Limited, part of Springer Nature. All rights reserved. In vitro models of the developing brain such as three-dimensional brain organoids offer an unprecedented opportunity to study aspects of human brain development and disease. However, the cells generated within organoi...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer Science and Business Media LLC
2021
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| Online Access: | https://hdl.handle.net/1721.1/135707 |
| _version_ | 1810978477242843136 |
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| author | Quadrato, Giorgia Nguyen, Tuan Macosko, Evan Z Sherwood, John L Min Yang, Sung Berger, Daniel R Maria, Natalie Scholvin, Jorg Goldman, Melissa Kinney, Justin P Boyden, Edward S Lichtman, Jeff W Williams, Ziv M McCarroll, Steven A Arlotta, Paola |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Quadrato, Giorgia Nguyen, Tuan Macosko, Evan Z Sherwood, John L Min Yang, Sung Berger, Daniel R Maria, Natalie Scholvin, Jorg Goldman, Melissa Kinney, Justin P Boyden, Edward S Lichtman, Jeff W Williams, Ziv M McCarroll, Steven A Arlotta, Paola |
| author_sort | Quadrato, Giorgia |
| collection | MIT |
| description | © 2017 Macmillan Publishers Limited, part of Springer Nature. All rights reserved. In vitro models of the developing brain such as three-dimensional brain organoids offer an unprecedented opportunity to study aspects of human brain development and disease. However, the cells generated within organoids and the extent to which they recapitulate the regional complexity, cellular diversity and circuit functionality of the brain remain undefined. Here we analyse gene expression in over 80,000 individual cells isolated from 31 human brain organoids. We find that organoids can generate a broad diversity of cells, which are related to endogenous classes, including cells from the cerebral cortex and the retina. Organoids could be developed over extended periods (more than 9 months), allowing for the establishment of relatively mature features, including the formation of dendritic spines and spontaneously active neuronal networks. Finally, neuronal activity within organoids could be controlled using light stimulation of photosensitive cells, which may offer a way to probe the functionality of human neuronal circuits using physiological sensory stimuli. |
| first_indexed | 2024-09-23T09:27:41Z |
| format | Article |
| id | mit-1721.1/135707 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T09:27:41Z |
| publishDate | 2021 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1357072023-09-06T20:20:35Z Cell diversity and network dynamics in photosensitive human brain organoids Quadrato, Giorgia Nguyen, Tuan Macosko, Evan Z Sherwood, John L Min Yang, Sung Berger, Daniel R Maria, Natalie Scholvin, Jorg Goldman, Melissa Kinney, Justin P Boyden, Edward S Lichtman, Jeff W Williams, Ziv M McCarroll, Steven A Arlotta, Paola Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Massachusetts Institute of Technology. Media Laboratory McGovern Institute for Brain Research at MIT © 2017 Macmillan Publishers Limited, part of Springer Nature. All rights reserved. In vitro models of the developing brain such as three-dimensional brain organoids offer an unprecedented opportunity to study aspects of human brain development and disease. However, the cells generated within organoids and the extent to which they recapitulate the regional complexity, cellular diversity and circuit functionality of the brain remain undefined. Here we analyse gene expression in over 80,000 individual cells isolated from 31 human brain organoids. We find that organoids can generate a broad diversity of cells, which are related to endogenous classes, including cells from the cerebral cortex and the retina. Organoids could be developed over extended periods (more than 9 months), allowing for the establishment of relatively mature features, including the formation of dendritic spines and spontaneously active neuronal networks. Finally, neuronal activity within organoids could be controlled using light stimulation of photosensitive cells, which may offer a way to probe the functionality of human neuronal circuits using physiological sensory stimuli. 2021-10-27T20:28:54Z 2021-10-27T20:28:54Z 2017 2019-07-19T12:17:07Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/135707 Quadrato, G., et al. "Cell Diversity and Network Dynamics in Photosensitive Human Brain Organoids." Nature (2017). en 10.1038/NATURE22047 Nature Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Springer Science and Business Media LLC PMC |
| spellingShingle | Quadrato, Giorgia Nguyen, Tuan Macosko, Evan Z Sherwood, John L Min Yang, Sung Berger, Daniel R Maria, Natalie Scholvin, Jorg Goldman, Melissa Kinney, Justin P Boyden, Edward S Lichtman, Jeff W Williams, Ziv M McCarroll, Steven A Arlotta, Paola Cell diversity and network dynamics in photosensitive human brain organoids |
| title | Cell diversity and network dynamics in photosensitive human brain organoids |
| title_full | Cell diversity and network dynamics in photosensitive human brain organoids |
| title_fullStr | Cell diversity and network dynamics in photosensitive human brain organoids |
| title_full_unstemmed | Cell diversity and network dynamics in photosensitive human brain organoids |
| title_short | Cell diversity and network dynamics in photosensitive human brain organoids |
| title_sort | cell diversity and network dynamics in photosensitive human brain organoids |
| url | https://hdl.handle.net/1721.1/135707 |
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