Expansion Mini-Microscopy: An Enabling Alternative in Point-of-Care Diagnostics
© 2017 Elsevier Inc. Diagnostics play a significant role in health care. In the developing world and low-resource regions the utility for point-of-care (POC) diagnostics becomes even greater. This need has long been recognized, and diagnostic technology has seen tremendous progress with the developm...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier BV
2021
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| Online Access: | https://hdl.handle.net/1721.1/135746 |
| _version_ | 1826217665385988096 |
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| author | Zhang, Yu Shrike Trujillo-de Santiago, Grissel Alvarez, Mario Moisés Schiff, Steven J Boyden, Edward S Khademhosseini, Ali |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Zhang, Yu Shrike Trujillo-de Santiago, Grissel Alvarez, Mario Moisés Schiff, Steven J Boyden, Edward S Khademhosseini, Ali |
| author_sort | Zhang, Yu Shrike |
| collection | MIT |
| description | © 2017 Elsevier Inc. Diagnostics play a significant role in health care. In the developing world and low-resource regions the utility for point-of-care (POC) diagnostics becomes even greater. This need has long been recognized, and diagnostic technology has seen tremendous progress with the development of portable instrumentation such as miniature imagers featuring low complexity and cost. However, such inexpensive devices have not been able to achieve a resolution sufficient for POC detection of pathogens at very small scales, such as single-cell parasites, bacteria, fungi, and viruses. To this end, expansion microscopy (ExM) is a recently developed technique that, by physically expanding preserved biological specimens through a chemical process, enables super-resolution imaging on conventional microscopes and improves imaging resolution of a given microscope without the need to modify the existing microscope hardware. Here we review recent advances in ExM and portable imagers, respectively, and discuss the rational combination of the two technologies, that we term expansion mini-microscopy (ExMM). In ExMM, the physical expansion of a biological sample followed by imaging on a mini-microscope achieves a resolution as high as that attainable by conventional high-end microscopes imaging non-expanded samples, at significant reduction in cost. We believe that this newly developed ExMM technique is likely to find widespread applications in POC diagnostics in resource-limited and remote regions by expanded-scale imaging of biological specimens that are otherwise not resolvable using low-cost imagers. |
| first_indexed | 2024-09-23T17:06:56Z |
| format | Article |
| id | mit-1721.1/135746 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T17:06:56Z |
| publishDate | 2021 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1357462023-01-20T20:27:56Z Expansion Mini-Microscopy: An Enabling Alternative in Point-of-Care Diagnostics Zhang, Yu Shrike Trujillo-de Santiago, Grissel Alvarez, Mario Moisés Schiff, Steven J Boyden, Edward S Khademhosseini, Ali Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Media Laboratory Massachusetts Institute of Technology. Department of Biological Engineering McGovern Institute for Brain Research at MIT Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Massachusetts Institute of Technology. Center for Neurobiological Engineering © 2017 Elsevier Inc. Diagnostics play a significant role in health care. In the developing world and low-resource regions the utility for point-of-care (POC) diagnostics becomes even greater. This need has long been recognized, and diagnostic technology has seen tremendous progress with the development of portable instrumentation such as miniature imagers featuring low complexity and cost. However, such inexpensive devices have not been able to achieve a resolution sufficient for POC detection of pathogens at very small scales, such as single-cell parasites, bacteria, fungi, and viruses. To this end, expansion microscopy (ExM) is a recently developed technique that, by physically expanding preserved biological specimens through a chemical process, enables super-resolution imaging on conventional microscopes and improves imaging resolution of a given microscope without the need to modify the existing microscope hardware. Here we review recent advances in ExM and portable imagers, respectively, and discuss the rational combination of the two technologies, that we term expansion mini-microscopy (ExMM). In ExMM, the physical expansion of a biological sample followed by imaging on a mini-microscope achieves a resolution as high as that attainable by conventional high-end microscopes imaging non-expanded samples, at significant reduction in cost. We believe that this newly developed ExMM technique is likely to find widespread applications in POC diagnostics in resource-limited and remote regions by expanded-scale imaging of biological specimens that are otherwise not resolvable using low-cost imagers. 2021-10-27T20:29:06Z 2021-10-27T20:29:06Z 2017 2019-07-19T12:35:55Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/135746 en 10.1016/J.COBME.2017.03.001 Current Opinion in Biomedical Engineering Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier BV PMC |
| spellingShingle | Zhang, Yu Shrike Trujillo-de Santiago, Grissel Alvarez, Mario Moisés Schiff, Steven J Boyden, Edward S Khademhosseini, Ali Expansion Mini-Microscopy: An Enabling Alternative in Point-of-Care Diagnostics |
| title | Expansion Mini-Microscopy: An Enabling Alternative in Point-of-Care Diagnostics |
| title_full | Expansion Mini-Microscopy: An Enabling Alternative in Point-of-Care Diagnostics |
| title_fullStr | Expansion Mini-Microscopy: An Enabling Alternative in Point-of-Care Diagnostics |
| title_full_unstemmed | Expansion Mini-Microscopy: An Enabling Alternative in Point-of-Care Diagnostics |
| title_short | Expansion Mini-Microscopy: An Enabling Alternative in Point-of-Care Diagnostics |
| title_sort | expansion mini microscopy an enabling alternative in point of care diagnostics |
| url | https://hdl.handle.net/1721.1/135746 |
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