Porous silicon–graphene oxide core–shell nanoparticles for targeted delivery of siRNA to the injured brain
© 2016 The Royal Society of Chemistry. We report the synthesis, characterization, and assessment of a nanoparticle-based RNAi delivery platform that protects siRNA payloads against nuclease-induced degradation and efficiently delivers them to target cells. The nanocarrier is based on biodegradable m...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Royal Society of Chemistry (RSC)
2021
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| Online Access: | https://hdl.handle.net/1721.1/135749 |
| _version_ | 1826199773742366720 |
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| author | Joo, Jinmyoung Kwon, Ester J Kang, Jinyoung Skalak, Matthew Anglin, Emily J Mann, Aman P Ruoslahti, Erkki Bhatia, Sangeeta N Sailor, Michael J |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Joo, Jinmyoung Kwon, Ester J Kang, Jinyoung Skalak, Matthew Anglin, Emily J Mann, Aman P Ruoslahti, Erkki Bhatia, Sangeeta N Sailor, Michael J |
| author_sort | Joo, Jinmyoung |
| collection | MIT |
| description | © 2016 The Royal Society of Chemistry. We report the synthesis, characterization, and assessment of a nanoparticle-based RNAi delivery platform that protects siRNA payloads against nuclease-induced degradation and efficiently delivers them to target cells. The nanocarrier is based on biodegradable mesoporous silicon nanoparticles (pSiNPs), where the voids of the nanoparticles are loaded with siRNA and the nanoparticles are encapsulated with graphene oxide nanosheets (GO-pSiNPs). The graphene oxide encapsulant delays release of the oligonucleotide payloads in vitro by a factor of 3. When conjugated to a targeting peptide derived from the rabies virus glycoprotein (RVG), the nanoparticles show 2-fold greater cellular uptake and gene silencing. Intravenous administration of the nanoparticles into brain-injured mice results in substantial accumulation specifically at the site of injury. |
| first_indexed | 2024-09-23T11:25:33Z |
| format | Article |
| id | mit-1721.1/135749 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T11:25:33Z |
| publishDate | 2021 |
| publisher | Royal Society of Chemistry (RSC) |
| record_format | dspace |
| spelling | mit-1721.1/1357492023-03-01T14:54:26Z Porous silicon–graphene oxide core–shell nanoparticles for targeted delivery of siRNA to the injured brain Joo, Jinmyoung Kwon, Ester J Kang, Jinyoung Skalak, Matthew Anglin, Emily J Mann, Aman P Ruoslahti, Erkki Bhatia, Sangeeta N Sailor, Michael J Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Koch Institute for Integrative Cancer Research at MIT © 2016 The Royal Society of Chemistry. We report the synthesis, characterization, and assessment of a nanoparticle-based RNAi delivery platform that protects siRNA payloads against nuclease-induced degradation and efficiently delivers them to target cells. The nanocarrier is based on biodegradable mesoporous silicon nanoparticles (pSiNPs), where the voids of the nanoparticles are loaded with siRNA and the nanoparticles are encapsulated with graphene oxide nanosheets (GO-pSiNPs). The graphene oxide encapsulant delays release of the oligonucleotide payloads in vitro by a factor of 3. When conjugated to a targeting peptide derived from the rabies virus glycoprotein (RVG), the nanoparticles show 2-fold greater cellular uptake and gene silencing. Intravenous administration of the nanoparticles into brain-injured mice results in substantial accumulation specifically at the site of injury. 2021-10-27T20:29:07Z 2021-10-27T20:29:07Z 2016 2019-05-09T16:08:03Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/135749 en 10.1039/C6NH00082G Nanoscale Horiz. Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Royal Society of Chemistry (RSC) PMC |
| spellingShingle | Joo, Jinmyoung Kwon, Ester J Kang, Jinyoung Skalak, Matthew Anglin, Emily J Mann, Aman P Ruoslahti, Erkki Bhatia, Sangeeta N Sailor, Michael J Porous silicon–graphene oxide core–shell nanoparticles for targeted delivery of siRNA to the injured brain |
| title | Porous silicon–graphene oxide core–shell nanoparticles for targeted delivery of siRNA to the injured brain |
| title_full | Porous silicon–graphene oxide core–shell nanoparticles for targeted delivery of siRNA to the injured brain |
| title_fullStr | Porous silicon–graphene oxide core–shell nanoparticles for targeted delivery of siRNA to the injured brain |
| title_full_unstemmed | Porous silicon–graphene oxide core–shell nanoparticles for targeted delivery of siRNA to the injured brain |
| title_short | Porous silicon–graphene oxide core–shell nanoparticles for targeted delivery of siRNA to the injured brain |
| title_sort | porous silicon graphene oxide core shell nanoparticles for targeted delivery of sirna to the injured brain |
| url | https://hdl.handle.net/1721.1/135749 |
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