Engineered microbial biofuel production and recovery under supercritical carbon dioxide
© 2019, The Author(s). Culture contamination, end-product toxicity, and energy efficient product recovery are long-standing bioprocess challenges. To solve these problems, we propose a high-pressure fermentation strategy, coupled with in situ extraction using the abundant and renewable solvent supe...
| Autori principali: | , , , , , , , , , |
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| Altri autori: | |
| Natura: | Articolo |
| Lingua: | English |
| Pubblicazione: |
Springer Science and Business Media LLC
2021
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| Accesso online: | https://hdl.handle.net/1721.1/135865 |
| _version_ | 1826194300554182656 |
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| author | Boock, Jason T Freedman, Adam JE Tompsett, Geoffrey A Muse, Sarah K Allen, Audrey J Jackson, Luke A Castro-Dominguez, Bernardo Timko, Michael T Prather, Kristala LJ Thompson, Janelle R |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Boock, Jason T Freedman, Adam JE Tompsett, Geoffrey A Muse, Sarah K Allen, Audrey J Jackson, Luke A Castro-Dominguez, Bernardo Timko, Michael T Prather, Kristala LJ Thompson, Janelle R |
| author_sort | Boock, Jason T |
| collection | MIT |
| description | © 2019, The Author(s). Culture contamination, end-product toxicity, and energy efficient product recovery are long-standing bioprocess challenges. To solve these problems, we propose a high-pressure fermentation strategy, coupled with in situ extraction using the abundant and renewable solvent supercritical carbon dioxide (scCO 2 ), which is also known for its broad microbial lethality. Towards this goal, we report the domestication and engineering of a scCO 2 -tolerant strain of Bacillus megaterium, previously isolated from formation waters from the McElmo Dome CO 2 field, to produce branched alcohols that have potential use as biofuels. After establishing induced-expression under scCO 2 , isobutanol production from 2-ketoisovalerate is observed with greater than 40% yield with co-produced isopentanol. Finally, we present a process model to compare the energy required for our process to other in situ extraction methods, such as gas stripping, finding scCO 2 extraction to be potentially competitive, if not superior. |
| first_indexed | 2024-09-23T09:53:55Z |
| format | Article |
| id | mit-1721.1/135865 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T09:53:55Z |
| publishDate | 2021 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1358652023-09-26T20:08:40Z Engineered microbial biofuel production and recovery under supercritical carbon dioxide Boock, Jason T Freedman, Adam JE Tompsett, Geoffrey A Muse, Sarah K Allen, Audrey J Jackson, Luke A Castro-Dominguez, Bernardo Timko, Michael T Prather, Kristala LJ Thompson, Janelle R Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Civil and Environmental Engineering © 2019, The Author(s). Culture contamination, end-product toxicity, and energy efficient product recovery are long-standing bioprocess challenges. To solve these problems, we propose a high-pressure fermentation strategy, coupled with in situ extraction using the abundant and renewable solvent supercritical carbon dioxide (scCO 2 ), which is also known for its broad microbial lethality. Towards this goal, we report the domestication and engineering of a scCO 2 -tolerant strain of Bacillus megaterium, previously isolated from formation waters from the McElmo Dome CO 2 field, to produce branched alcohols that have potential use as biofuels. After establishing induced-expression under scCO 2 , isobutanol production from 2-ketoisovalerate is observed with greater than 40% yield with co-produced isopentanol. Finally, we present a process model to compare the energy required for our process to other in situ extraction methods, such as gas stripping, finding scCO 2 extraction to be potentially competitive, if not superior. 2021-10-27T20:29:42Z 2021-10-27T20:29:42Z 2019 2019-07-18T14:09:57Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/135865 en 10.1038/S41467-019-08486-6 Nature Communications Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Springer Science and Business Media LLC Nature |
| spellingShingle | Boock, Jason T Freedman, Adam JE Tompsett, Geoffrey A Muse, Sarah K Allen, Audrey J Jackson, Luke A Castro-Dominguez, Bernardo Timko, Michael T Prather, Kristala LJ Thompson, Janelle R Engineered microbial biofuel production and recovery under supercritical carbon dioxide |
| title | Engineered microbial biofuel production and recovery under supercritical carbon dioxide |
| title_full | Engineered microbial biofuel production and recovery under supercritical carbon dioxide |
| title_fullStr | Engineered microbial biofuel production and recovery under supercritical carbon dioxide |
| title_full_unstemmed | Engineered microbial biofuel production and recovery under supercritical carbon dioxide |
| title_short | Engineered microbial biofuel production and recovery under supercritical carbon dioxide |
| title_sort | engineered microbial biofuel production and recovery under supercritical carbon dioxide |
| url | https://hdl.handle.net/1721.1/135865 |
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