New observations in neuroscience using superresolution microscopy
© 2018 the authors. Superresolution microscopy (SM) techniques are among the revolutionary methods for molecular and cellular observations in the 21st century. SM techniques overcome optical limitations, and several new observations using SM lead us to expect these techniques to have a large impact...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Society for Neuroscience
2021
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| Online Access: | https://hdl.handle.net/1721.1/136375 |
| _version_ | 1826189299420233728 |
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| author | Igarashi, Michihiro Nozumi, Motohiro Wu, Ling-Gang Cella Zanacchi, Francesca Katona, István Barna, László Xu, Pingyong Zhang, Mingshu Xue, Fudong Boyden, Edward |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Igarashi, Michihiro Nozumi, Motohiro Wu, Ling-Gang Cella Zanacchi, Francesca Katona, István Barna, László Xu, Pingyong Zhang, Mingshu Xue, Fudong Boyden, Edward |
| author_sort | Igarashi, Michihiro |
| collection | MIT |
| description | © 2018 the authors. Superresolution microscopy (SM) techniques are among the revolutionary methods for molecular and cellular observations in the 21st century. SM techniques overcome optical limitations, and several new observations using SM lead us to expect these techniques to have a large impact on neuroscience in the near future. Several types of SM have been developed, including structured illumination microscopy (SIM), stimulated emission depletion microscopy (STED), and photoactivated localization microscopy (PALM)/stochastic optical reconstruction microscopy (STORM), each with special features. In this Minisymposium, experts in these different types of SM discuss the new structural and functional information about specific important molecules in neuroscience that has been gained with SM. Using these techniques, we have revealed novel mechanisms of endocytosis in nerve growth, fusion pore dynamics, and described quantitative new properties of excitatory and inhibitory synapses. Additional powerful techniques, including single molecule-guided Bayesian localization SM (SIMBA) and expansion microscopy (ExM), alone or combined with super-resolution observation, are also introduced in this session. |
| first_indexed | 2024-09-23T08:12:54Z |
| format | Article |
| id | mit-1721.1/136375 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:12:54Z |
| publishDate | 2021 |
| publisher | Society for Neuroscience |
| record_format | dspace |
| spelling | mit-1721.1/1363752023-02-22T21:16:27Z New observations in neuroscience using superresolution microscopy Igarashi, Michihiro Nozumi, Motohiro Wu, Ling-Gang Cella Zanacchi, Francesca Katona, István Barna, László Xu, Pingyong Zhang, Mingshu Xue, Fudong Boyden, Edward Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Massachusetts Institute of Technology. Media Laboratory © 2018 the authors. Superresolution microscopy (SM) techniques are among the revolutionary methods for molecular and cellular observations in the 21st century. SM techniques overcome optical limitations, and several new observations using SM lead us to expect these techniques to have a large impact on neuroscience in the near future. Several types of SM have been developed, including structured illumination microscopy (SIM), stimulated emission depletion microscopy (STED), and photoactivated localization microscopy (PALM)/stochastic optical reconstruction microscopy (STORM), each with special features. In this Minisymposium, experts in these different types of SM discuss the new structural and functional information about specific important molecules in neuroscience that has been gained with SM. Using these techniques, we have revealed novel mechanisms of endocytosis in nerve growth, fusion pore dynamics, and described quantitative new properties of excitatory and inhibitory synapses. Additional powerful techniques, including single molecule-guided Bayesian localization SM (SIMBA) and expansion microscopy (ExM), alone or combined with super-resolution observation, are also introduced in this session. 2021-10-27T20:35:05Z 2021-10-27T20:35:05Z 2018 2019-07-19T16:10:37Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/136375 en 10.1523/JNEUROSCI.1678-18.2018 Journal of Neuroscience Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Society for Neuroscience Society for Neurocience |
| spellingShingle | Igarashi, Michihiro Nozumi, Motohiro Wu, Ling-Gang Cella Zanacchi, Francesca Katona, István Barna, László Xu, Pingyong Zhang, Mingshu Xue, Fudong Boyden, Edward New observations in neuroscience using superresolution microscopy |
| title | New observations in neuroscience using superresolution microscopy |
| title_full | New observations in neuroscience using superresolution microscopy |
| title_fullStr | New observations in neuroscience using superresolution microscopy |
| title_full_unstemmed | New observations in neuroscience using superresolution microscopy |
| title_short | New observations in neuroscience using superresolution microscopy |
| title_sort | new observations in neuroscience using superresolution microscopy |
| url | https://hdl.handle.net/1721.1/136375 |
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