Immuno-SABER enables highly multiplexed and amplified protein imaging in tissues
© 2019, The Author(s), under exclusive licence to Springer Nature America, Inc. Spatial mapping of proteins in tissues is hindered by limitations in multiplexing, sensitivity and throughput. Here we report immunostaining with signal amplification by exchange reaction (Immuno-SABER), which achieves h...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Springer Science and Business Media LLC
2021
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| Online Access: | https://hdl.handle.net/1721.1/136430 |
| _version_ | 1826189031715635200 |
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| author | Saka, Sinem K Wang, Yu Kishi, Jocelyn Y Zhu, Allen Zeng, Yitian Xie, Wenxin Kirli, Koray Yapp, Clarence Cicconet, Marcelo Beliveau, Brian J Lapan, Sylvain W Yin, Siyuan Lin, Millicent Boyden, Edward S Kaeser, Pascal S Pihan, German Church, George M Yin, Peng |
| author2 | Massachusetts Institute of Technology. Media Laboratory |
| author_facet | Massachusetts Institute of Technology. Media Laboratory Saka, Sinem K Wang, Yu Kishi, Jocelyn Y Zhu, Allen Zeng, Yitian Xie, Wenxin Kirli, Koray Yapp, Clarence Cicconet, Marcelo Beliveau, Brian J Lapan, Sylvain W Yin, Siyuan Lin, Millicent Boyden, Edward S Kaeser, Pascal S Pihan, German Church, George M Yin, Peng |
| author_sort | Saka, Sinem K |
| collection | MIT |
| description | © 2019, The Author(s), under exclusive licence to Springer Nature America, Inc. Spatial mapping of proteins in tissues is hindered by limitations in multiplexing, sensitivity and throughput. Here we report immunostaining with signal amplification by exchange reaction (Immuno-SABER), which achieves highly multiplexed signal amplification via DNA-barcoded antibodies and orthogonal DNA concatemers generated by primer exchange reaction (PER). SABER offers independently programmable signal amplification without in situ enzymatic reactions, and intrinsic scalability to rapidly amplify and visualize a large number of targets when combined with fast exchange cycles of fluorescent imager strands. We demonstrate 5- to 180-fold signal amplification in diverse samples (cultured cells, cryosections, formalin-fixed paraffin-embedded sections and whole-mount tissues), as well as simultaneous signal amplification for ten different proteins using standard equipment and workflows. We also combined SABER with expansion microscopy to enable rapid, multiplexed super-resolution tissue imaging. Immuno-SABER presents an effective and accessible platform for multiplexed and amplified imaging of proteins with high sensitivity and throughput. |
| first_indexed | 2024-09-23T08:08:30Z |
| format | Article |
| id | mit-1721.1/136430 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:08:30Z |
| publishDate | 2021 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1364302023-12-08T21:16:12Z Immuno-SABER enables highly multiplexed and amplified protein imaging in tissues Saka, Sinem K Wang, Yu Kishi, Jocelyn Y Zhu, Allen Zeng, Yitian Xie, Wenxin Kirli, Koray Yapp, Clarence Cicconet, Marcelo Beliveau, Brian J Lapan, Sylvain W Yin, Siyuan Lin, Millicent Boyden, Edward S Kaeser, Pascal S Pihan, German Church, George M Yin, Peng Massachusetts Institute of Technology. Media Laboratory © 2019, The Author(s), under exclusive licence to Springer Nature America, Inc. Spatial mapping of proteins in tissues is hindered by limitations in multiplexing, sensitivity and throughput. Here we report immunostaining with signal amplification by exchange reaction (Immuno-SABER), which achieves highly multiplexed signal amplification via DNA-barcoded antibodies and orthogonal DNA concatemers generated by primer exchange reaction (PER). SABER offers independently programmable signal amplification without in situ enzymatic reactions, and intrinsic scalability to rapidly amplify and visualize a large number of targets when combined with fast exchange cycles of fluorescent imager strands. We demonstrate 5- to 180-fold signal amplification in diverse samples (cultured cells, cryosections, formalin-fixed paraffin-embedded sections and whole-mount tissues), as well as simultaneous signal amplification for ten different proteins using standard equipment and workflows. We also combined SABER with expansion microscopy to enable rapid, multiplexed super-resolution tissue imaging. Immuno-SABER presents an effective and accessible platform for multiplexed and amplified imaging of proteins with high sensitivity and throughput. 2021-10-27T20:35:20Z 2021-10-27T20:35:20Z 2019 2021-03-11T18:50:05Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/136430 en 10.1038/S41587-019-0207-Y Nature Biotechnology Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Springer Science and Business Media LLC PMC |
| spellingShingle | Saka, Sinem K Wang, Yu Kishi, Jocelyn Y Zhu, Allen Zeng, Yitian Xie, Wenxin Kirli, Koray Yapp, Clarence Cicconet, Marcelo Beliveau, Brian J Lapan, Sylvain W Yin, Siyuan Lin, Millicent Boyden, Edward S Kaeser, Pascal S Pihan, German Church, George M Yin, Peng Immuno-SABER enables highly multiplexed and amplified protein imaging in tissues |
| title | Immuno-SABER enables highly multiplexed and amplified protein imaging in tissues |
| title_full | Immuno-SABER enables highly multiplexed and amplified protein imaging in tissues |
| title_fullStr | Immuno-SABER enables highly multiplexed and amplified protein imaging in tissues |
| title_full_unstemmed | Immuno-SABER enables highly multiplexed and amplified protein imaging in tissues |
| title_short | Immuno-SABER enables highly multiplexed and amplified protein imaging in tissues |
| title_sort | immuno saber enables highly multiplexed and amplified protein imaging in tissues |
| url | https://hdl.handle.net/1721.1/136430 |
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