Intravital imaging of mouse embryos
© 2020 The Authors. Embryonic development is a complex process that is unamenable to direct observation. In this study, we implanted a window to the mouse uterus to visualize the developing embryo from embryonic day 9.5 to birth. This removable intravital window allowed manipulation and high-resolut...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Association for the Advancement of Science (AAAS)
2021
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| Online Access: | https://hdl.handle.net/1721.1/136659 |
| _version_ | 1826207418527252480 |
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| author | Huang, Qiang Cohen, Malkiel A Alsina, Fernando C Devlin, Garth Garrett, Aliesha McKey, Jennifer Havlik, Patrick Rakhilin, Nikolai Wang, Ergang Xiang, Kun Mathews, Parker Wang, Lihua Bock, Cheryl Ruthig, Victor Wang, Yi Negrete, Marcos Wong, Chi Wut Murthy, Preetish KL Zhang, Shupei Daniel, Andrea R Kirsch, David G Kang, Yubin Capel, Blanche Asokan, Aravind Silver, Debra L Jaenisch, Rudolf Shen, Xiling |
| author_facet | Huang, Qiang Cohen, Malkiel A Alsina, Fernando C Devlin, Garth Garrett, Aliesha McKey, Jennifer Havlik, Patrick Rakhilin, Nikolai Wang, Ergang Xiang, Kun Mathews, Parker Wang, Lihua Bock, Cheryl Ruthig, Victor Wang, Yi Negrete, Marcos Wong, Chi Wut Murthy, Preetish KL Zhang, Shupei Daniel, Andrea R Kirsch, David G Kang, Yubin Capel, Blanche Asokan, Aravind Silver, Debra L Jaenisch, Rudolf Shen, Xiling |
| author_sort | Huang, Qiang |
| collection | MIT |
| description | © 2020 The Authors. Embryonic development is a complex process that is unamenable to direct observation. In this study, we implanted a window to the mouse uterus to visualize the developing embryo from embryonic day 9.5 to birth. This removable intravital window allowed manipulation and high-resolution imaging. In live mouse embryos, we observed transient neurotransmission and early vascularization of neural crest cell (NCC)-derived perivascular cells in the brain, autophagy in the retina, viral gene delivery, and chemical diffusion through the placenta. We combined the imaging window with in utero electroporation to label and track cell division and movement within embryos and observed that clusters of mouse NCC-derived cells expanded in interspecies chimeras, whereas adjacent human donor NCC-derived cells shrank. This technique can be combined with various tissue manipulation and microscopy methods to study the processes of development at unprecedented spatiotemporal resolution. |
| first_indexed | 2024-09-23T13:49:21Z |
| format | Article |
| id | mit-1721.1/136659 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T13:49:21Z |
| publishDate | 2021 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | dspace |
| spelling | mit-1721.1/1366592021-10-28T03:47:46Z Intravital imaging of mouse embryos Huang, Qiang Cohen, Malkiel A Alsina, Fernando C Devlin, Garth Garrett, Aliesha McKey, Jennifer Havlik, Patrick Rakhilin, Nikolai Wang, Ergang Xiang, Kun Mathews, Parker Wang, Lihua Bock, Cheryl Ruthig, Victor Wang, Yi Negrete, Marcos Wong, Chi Wut Murthy, Preetish KL Zhang, Shupei Daniel, Andrea R Kirsch, David G Kang, Yubin Capel, Blanche Asokan, Aravind Silver, Debra L Jaenisch, Rudolf Shen, Xiling © 2020 The Authors. Embryonic development is a complex process that is unamenable to direct observation. In this study, we implanted a window to the mouse uterus to visualize the developing embryo from embryonic day 9.5 to birth. This removable intravital window allowed manipulation and high-resolution imaging. In live mouse embryos, we observed transient neurotransmission and early vascularization of neural crest cell (NCC)-derived perivascular cells in the brain, autophagy in the retina, viral gene delivery, and chemical diffusion through the placenta. We combined the imaging window with in utero electroporation to label and track cell division and movement within embryos and observed that clusters of mouse NCC-derived cells expanded in interspecies chimeras, whereas adjacent human donor NCC-derived cells shrank. This technique can be combined with various tissue manipulation and microscopy methods to study the processes of development at unprecedented spatiotemporal resolution. 2021-10-27T20:36:28Z 2021-10-27T20:36:28Z 2020 2021-07-19T17:36:41Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/136659 en 10.1126/SCIENCE.ABA0210 Science Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf American Association for the Advancement of Science (AAAS) PMC |
| spellingShingle | Huang, Qiang Cohen, Malkiel A Alsina, Fernando C Devlin, Garth Garrett, Aliesha McKey, Jennifer Havlik, Patrick Rakhilin, Nikolai Wang, Ergang Xiang, Kun Mathews, Parker Wang, Lihua Bock, Cheryl Ruthig, Victor Wang, Yi Negrete, Marcos Wong, Chi Wut Murthy, Preetish KL Zhang, Shupei Daniel, Andrea R Kirsch, David G Kang, Yubin Capel, Blanche Asokan, Aravind Silver, Debra L Jaenisch, Rudolf Shen, Xiling Intravital imaging of mouse embryos |
| title | Intravital imaging of mouse embryos |
| title_full | Intravital imaging of mouse embryos |
| title_fullStr | Intravital imaging of mouse embryos |
| title_full_unstemmed | Intravital imaging of mouse embryos |
| title_short | Intravital imaging of mouse embryos |
| title_sort | intravital imaging of mouse embryos |
| url | https://hdl.handle.net/1721.1/136659 |
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