Helicobacter cyclurae sp. Nov., Isolated From Endangered Blue Iguanas (Cyclura lewisi)
<jats:p>Blue iguanas (<jats:italic>Cyclura lewisi</jats:italic>) are endangered reptiles found only on Grand Cayman. Previously, DNA for a novel <jats:italic>Helicobacter</jats:italic> species GCBI1 was detected in sick and dead iguanas. In the current study, fecal and...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media SA
2021
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| Online Access: | https://hdl.handle.net/1721.1/137224 |
| _version_ | 1826198151831224320 |
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| author | Chan, Nathan Shen, Zeli Mannion, Anthony Kurnick, Susanna Popescu, Ioana S Burton, Frederic J Calle, Paul P Fox, James G |
| author2 | Massachusetts Institute of Technology. Division of Comparative Medicine |
| author_facet | Massachusetts Institute of Technology. Division of Comparative Medicine Chan, Nathan Shen, Zeli Mannion, Anthony Kurnick, Susanna Popescu, Ioana S Burton, Frederic J Calle, Paul P Fox, James G |
| author_sort | Chan, Nathan |
| collection | MIT |
| description | <jats:p>Blue iguanas (<jats:italic>Cyclura lewisi</jats:italic>) are endangered reptiles found only on Grand Cayman. Previously, DNA for a novel <jats:italic>Helicobacter</jats:italic> species GCBI1 was detected in sick and dead iguanas. In the current study, fecal and cloacal swab samples were obtained from 25 iguanas. Through molecular and microbiological techniques, a novel <jats:italic>Helicobacter</jats:italic> species was cultured from feces and characterized, for whom we propose the name <jats:italic>Helicobacter cyclurae</jats:italic>. This novel helicobacter had a prevalence of 56% by PCR and 20% by culture in samples analyzed. The type strain MIT 16-1353 was catalase, oxidase, and gamma-glutamyl transpeptidase positive. By electron microscopy, <jats:italic>H. cyclurae</jats:italic> has a curved rod morphology and a single sheathed polar flagellum. Phylogenetic analysis using 16S rRNA, <jats:italic>gyr</jats:italic>B, and <jats:italic>hsp</jats:italic>60 indicated that these strains were most closely related to <jats:italic>Helicobacter</jats:italic> sp. 12502256-12 previously isolated from lizards. <jats:italic>H. cyclurae</jats:italic> has a 1.91-Mb genome with a GC content of 33.37%. There were 1,969 genes with four notable virulence genes: high temperature requirement-A protein-secreted serine protease, gamma-glutamyl transpeptidase, fibronectin/fibrinogen binding protein, and neutrophil-activating protein. Whole-genome phylogeny, average nucleotide identity, and digital DNA–DNA hybridization analysis confirmed that <jats:italic>H. cyclurae</jats:italic> is a novel species, and the first helicobacter cultured and characterized from blue iguanas.</jats:p> |
| first_indexed | 2024-09-23T10:59:49Z |
| format | Article |
| id | mit-1721.1/137224 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T10:59:49Z |
| publishDate | 2021 |
| publisher | Frontiers Media SA |
| record_format | dspace |
| spelling | mit-1721.1/1372242023-02-09T15:53:00Z Helicobacter cyclurae sp. Nov., Isolated From Endangered Blue Iguanas (Cyclura lewisi) Chan, Nathan Shen, Zeli Mannion, Anthony Kurnick, Susanna Popescu, Ioana S Burton, Frederic J Calle, Paul P Fox, James G Massachusetts Institute of Technology. Division of Comparative Medicine <jats:p>Blue iguanas (<jats:italic>Cyclura lewisi</jats:italic>) are endangered reptiles found only on Grand Cayman. Previously, DNA for a novel <jats:italic>Helicobacter</jats:italic> species GCBI1 was detected in sick and dead iguanas. In the current study, fecal and cloacal swab samples were obtained from 25 iguanas. Through molecular and microbiological techniques, a novel <jats:italic>Helicobacter</jats:italic> species was cultured from feces and characterized, for whom we propose the name <jats:italic>Helicobacter cyclurae</jats:italic>. This novel helicobacter had a prevalence of 56% by PCR and 20% by culture in samples analyzed. The type strain MIT 16-1353 was catalase, oxidase, and gamma-glutamyl transpeptidase positive. By electron microscopy, <jats:italic>H. cyclurae</jats:italic> has a curved rod morphology and a single sheathed polar flagellum. Phylogenetic analysis using 16S rRNA, <jats:italic>gyr</jats:italic>B, and <jats:italic>hsp</jats:italic>60 indicated that these strains were most closely related to <jats:italic>Helicobacter</jats:italic> sp. 12502256-12 previously isolated from lizards. <jats:italic>H. cyclurae</jats:italic> has a 1.91-Mb genome with a GC content of 33.37%. There were 1,969 genes with four notable virulence genes: high temperature requirement-A protein-secreted serine protease, gamma-glutamyl transpeptidase, fibronectin/fibrinogen binding protein, and neutrophil-activating protein. Whole-genome phylogeny, average nucleotide identity, and digital DNA–DNA hybridization analysis confirmed that <jats:italic>H. cyclurae</jats:italic> is a novel species, and the first helicobacter cultured and characterized from blue iguanas.</jats:p> 2021-11-03T15:50:54Z 2021-11-03T15:50:54Z 2021-09-14 2021-11-03T15:43:00Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/137224 Chan, Nathan, Shen, Zeli, Mannion, Anthony, Kurnick, Susanna, Popescu, Ioana S et al. 2021. "Helicobacter cyclurae sp. Nov., Isolated From Endangered Blue Iguanas (Cyclura lewisi)." Frontiers in Ecology and Evolution, 9. en 10.3389/fevo.2021.676682 Frontiers in Ecology and Evolution Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Frontiers Media SA Frontiers |
| spellingShingle | Chan, Nathan Shen, Zeli Mannion, Anthony Kurnick, Susanna Popescu, Ioana S Burton, Frederic J Calle, Paul P Fox, James G Helicobacter cyclurae sp. Nov., Isolated From Endangered Blue Iguanas (Cyclura lewisi) |
| title | Helicobacter cyclurae sp. Nov., Isolated From Endangered Blue Iguanas (Cyclura lewisi) |
| title_full | Helicobacter cyclurae sp. Nov., Isolated From Endangered Blue Iguanas (Cyclura lewisi) |
| title_fullStr | Helicobacter cyclurae sp. Nov., Isolated From Endangered Blue Iguanas (Cyclura lewisi) |
| title_full_unstemmed | Helicobacter cyclurae sp. Nov., Isolated From Endangered Blue Iguanas (Cyclura lewisi) |
| title_short | Helicobacter cyclurae sp. Nov., Isolated From Endangered Blue Iguanas (Cyclura lewisi) |
| title_sort | helicobacter cyclurae sp nov isolated from endangered blue iguanas cyclura lewisi |
| url | https://hdl.handle.net/1721.1/137224 |
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