Rapid Detection and Quantification of Plant Innate Immunity Response Using Raman Spectroscopy
<jats:p>We have developed a rapid Raman spectroscopy-based method for the detection and quantification of early innate immunity responses in Arabidopsis and Choy Sum plants. Arabidopsis plants challenged with flg22 and elf18 elicitors could be differentiated from mock-treated plants by their R...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
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Frontiers Media SA
2021
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| Online Access: | https://hdl.handle.net/1721.1/137239 |
| _version_ | 1826202354954797056 |
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| author | Chung, Pil Joong Singh, Gajendra P. Huang, Chung-Hao Koyyappurath, Sayuj Seo, Jun Sung Mao, Hui-Zhu Diloknawarit, Piyarut Ram, Rajeev J. Sarojam, Rajani Chua, Nam-Hai |
| author2 | Singapore-MIT Alliance in Research and Technology (SMART) |
| author_facet | Singapore-MIT Alliance in Research and Technology (SMART) Chung, Pil Joong Singh, Gajendra P. Huang, Chung-Hao Koyyappurath, Sayuj Seo, Jun Sung Mao, Hui-Zhu Diloknawarit, Piyarut Ram, Rajeev J. Sarojam, Rajani Chua, Nam-Hai |
| author_sort | Chung, Pil Joong |
| collection | MIT |
| description | <jats:p>We have developed a rapid Raman spectroscopy-based method for the detection and quantification of early innate immunity responses in Arabidopsis and Choy Sum plants. Arabidopsis plants challenged with flg22 and elf18 elicitors could be differentiated from mock-treated plants by their Raman spectral fingerprints. From the difference Raman spectrum and the value of <jats:italic>p</jats:italic> at each Raman shift, we derived the Elicitor Response Index (ERI) as a quantitative measure of the response whereby a higher ERI value indicates a more significant elicitor-induced immune response. Among various Raman spectral bands contributing toward the ERI value, the most significant changes were observed in those associated with carotenoids and proteins. To validate these results, we investigated several characterized Arabidopsis pattern-triggered immunity (PTI) mutants. Compared to wild type (WT), positive regulatory mutants had ERI values close to zero, whereas negative regulatory mutants at early time points had higher ERI values. Similar to elicitor treatments, we derived an analogous Infection Response Index (IRI) as a quantitative measure to detect the early PTI response in Arabidopsis and Choy Sum plants infected with bacterial pathogens. The Raman spectral bands contributing toward a high IRI value were largely identical to the ERI Raman spectral bands. Raman spectroscopy is a convenient tool for rapid screening for Arabidopsis PTI mutants and may be suitable for the noninvasive and early diagnosis of pathogen-infected crop plants.</jats:p> |
| first_indexed | 2024-09-23T12:06:09Z |
| format | Article |
| id | mit-1721.1/137239 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T12:06:09Z |
| publishDate | 2021 |
| publisher | Frontiers Media SA |
| record_format | dspace |
| spelling | mit-1721.1/1372392023-06-26T20:01:26Z Rapid Detection and Quantification of Plant Innate Immunity Response Using Raman Spectroscopy Chung, Pil Joong Singh, Gajendra P. Huang, Chung-Hao Koyyappurath, Sayuj Seo, Jun Sung Mao, Hui-Zhu Diloknawarit, Piyarut Ram, Rajeev J. Sarojam, Rajani Chua, Nam-Hai Singapore-MIT Alliance in Research and Technology (SMART) Massachusetts Institute of Technology. Research Laboratory of Electronics <jats:p>We have developed a rapid Raman spectroscopy-based method for the detection and quantification of early innate immunity responses in Arabidopsis and Choy Sum plants. Arabidopsis plants challenged with flg22 and elf18 elicitors could be differentiated from mock-treated plants by their Raman spectral fingerprints. From the difference Raman spectrum and the value of <jats:italic>p</jats:italic> at each Raman shift, we derived the Elicitor Response Index (ERI) as a quantitative measure of the response whereby a higher ERI value indicates a more significant elicitor-induced immune response. Among various Raman spectral bands contributing toward the ERI value, the most significant changes were observed in those associated with carotenoids and proteins. To validate these results, we investigated several characterized Arabidopsis pattern-triggered immunity (PTI) mutants. Compared to wild type (WT), positive regulatory mutants had ERI values close to zero, whereas negative regulatory mutants at early time points had higher ERI values. Similar to elicitor treatments, we derived an analogous Infection Response Index (IRI) as a quantitative measure to detect the early PTI response in Arabidopsis and Choy Sum plants infected with bacterial pathogens. The Raman spectral bands contributing toward a high IRI value were largely identical to the ERI Raman spectral bands. Raman spectroscopy is a convenient tool for rapid screening for Arabidopsis PTI mutants and may be suitable for the noninvasive and early diagnosis of pathogen-infected crop plants.</jats:p> 2021-11-03T16:52:25Z 2021-11-03T16:52:25Z 2021-10-21 Article http://purl.org/eprint/type/JournalArticle 1664-462X https://hdl.handle.net/1721.1/137239 Chung, Pil Joong, Singh, Gajendra P., Huang, Chung-Hao, Koyyappurath, Sayuj, Seo, Jun Sung et al. 2021. "Rapid Detection and Quantification of Plant Innate Immunity Response Using Raman Spectroscopy." 12. 10.3389/fpls.2021.746586 Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Frontiers Media SA Frontiers |
| spellingShingle | Chung, Pil Joong Singh, Gajendra P. Huang, Chung-Hao Koyyappurath, Sayuj Seo, Jun Sung Mao, Hui-Zhu Diloknawarit, Piyarut Ram, Rajeev J. Sarojam, Rajani Chua, Nam-Hai Rapid Detection and Quantification of Plant Innate Immunity Response Using Raman Spectroscopy |
| title | Rapid Detection and Quantification of Plant Innate Immunity Response Using Raman Spectroscopy |
| title_full | Rapid Detection and Quantification of Plant Innate Immunity Response Using Raman Spectroscopy |
| title_fullStr | Rapid Detection and Quantification of Plant Innate Immunity Response Using Raman Spectroscopy |
| title_full_unstemmed | Rapid Detection and Quantification of Plant Innate Immunity Response Using Raman Spectroscopy |
| title_short | Rapid Detection and Quantification of Plant Innate Immunity Response Using Raman Spectroscopy |
| title_sort | rapid detection and quantification of plant innate immunity response using raman spectroscopy |
| url | https://hdl.handle.net/1721.1/137239 |
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