Multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues
© 2020 SPIE. The formation and accumulation of advanced glycation end products (AGEs) contribute to diabetic complications such as retinopathy, neuropathy, nephropathy, and cardiovascular diseases. It is clear that the development of effective technique in AGEs detection and the establishing the cor...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SPIE
2021
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| Online Access: | https://hdl.handle.net/1721.1/137474.2 |
| _version_ | 1811075436343459840 |
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| author | Lin, Chih-Ju Kang, Jeon Woong So, Peter T. C. Dong, Chen-Yuan |
| author2 | Massachusetts Institute of Technology. Laser Biomedical Research Center |
| author_facet | Massachusetts Institute of Technology. Laser Biomedical Research Center Lin, Chih-Ju Kang, Jeon Woong So, Peter T. C. Dong, Chen-Yuan |
| author_sort | Lin, Chih-Ju |
| collection | MIT |
| description | © 2020 SPIE. The formation and accumulation of advanced glycation end products (AGEs) contribute to diabetic complications such as retinopathy, neuropathy, nephropathy, and cardiovascular diseases. It is clear that the development of effective technique in AGEs detection and the establishing the correlation of measured AGE parameters to diabetic pathogenesis are invaluable in the monitoring of disease progression and drug discovery of anti-AGE compounds. Since some AGE are fluorescent, we propose to investigate the degree of tissue glycation in forming fluorescent AGEs (fAGEs). In this preliminary study, we will investigate the effects of glucose, fructose, and galactose, three of the most abundant dietary simple sugars, in fAGEs production. Excised tissues will be treated in solutions containing the three sugar types; multiphoton autofluorescence imaging will then be performed on the treated tissues to determine their autofluroescence levels. |
| first_indexed | 2024-09-23T10:05:51Z |
| format | Article |
| id | mit-1721.1/137474.2 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T10:05:51Z |
| publishDate | 2021 |
| publisher | SPIE |
| record_format | dspace |
| spelling | mit-1721.1/137474.22021-11-24T18:25:30Z Multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues Lin, Chih-Ju Kang, Jeon Woong So, Peter T. C. Dong, Chen-Yuan Massachusetts Institute of Technology. Laser Biomedical Research Center Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Department of Biological Engineering © 2020 SPIE. The formation and accumulation of advanced glycation end products (AGEs) contribute to diabetic complications such as retinopathy, neuropathy, nephropathy, and cardiovascular diseases. It is clear that the development of effective technique in AGEs detection and the establishing the correlation of measured AGE parameters to diabetic pathogenesis are invaluable in the monitoring of disease progression and drug discovery of anti-AGE compounds. Since some AGE are fluorescent, we propose to investigate the degree of tissue glycation in forming fluorescent AGEs (fAGEs). In this preliminary study, we will investigate the effects of glucose, fructose, and galactose, three of the most abundant dietary simple sugars, in fAGEs production. Excised tissues will be treated in solutions containing the three sugar types; multiphoton autofluorescence imaging will then be performed on the treated tissues to determine their autofluroescence levels. 2021-11-24T18:25:29Z 2021-11-05T14:19:59Z 2021-11-24T18:25:29Z 2020-02 2020-08-10T15:30:20Z Article http://purl.org/eprint/type/ConferencePaper https://hdl.handle.net/1721.1/137474.2 Lin, Chih-Ju, Kang, Jeon Woong, So, Peter T. C. and Dong, Chen-Yuan. 2020. "Multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues." Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 11244. en 10.1117/12.2545548 Progress in Biomedical Optics and Imaging - Proceedings of SPIE Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/octet-stream SPIE SPIE |
| spellingShingle | Lin, Chih-Ju Kang, Jeon Woong So, Peter T. C. Dong, Chen-Yuan Multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues |
| title | Multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues |
| title_full | Multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues |
| title_fullStr | Multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues |
| title_full_unstemmed | Multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues |
| title_short | Multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues |
| title_sort | multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues |
| url | https://hdl.handle.net/1721.1/137474.2 |
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