Multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues

© 2020 SPIE. The formation and accumulation of advanced glycation end products (AGEs) contribute to diabetic complications such as retinopathy, neuropathy, nephropathy, and cardiovascular diseases. It is clear that the development of effective technique in AGEs detection and the establishing the cor...

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Main Authors: Lin, Chih-Ju, Kang, Jeon Woong, So, Peter T. C., Dong, Chen-Yuan
Other Authors: Massachusetts Institute of Technology. Laser Biomedical Research Center
Format: Article
Language:English
Published: SPIE 2021
Online Access:https://hdl.handle.net/1721.1/137474
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author Lin, Chih-Ju
Kang, Jeon Woong
So, Peter T. C.
Dong, Chen-Yuan
author2 Massachusetts Institute of Technology. Laser Biomedical Research Center
author_facet Massachusetts Institute of Technology. Laser Biomedical Research Center
Lin, Chih-Ju
Kang, Jeon Woong
So, Peter T. C.
Dong, Chen-Yuan
author_sort Lin, Chih-Ju
collection MIT
description © 2020 SPIE. The formation and accumulation of advanced glycation end products (AGEs) contribute to diabetic complications such as retinopathy, neuropathy, nephropathy, and cardiovascular diseases. It is clear that the development of effective technique in AGEs detection and the establishing the correlation of measured AGE parameters to diabetic pathogenesis are invaluable in the monitoring of disease progression and drug discovery of anti-AGE compounds. Since some AGE are fluorescent, we propose to investigate the degree of tissue glycation in forming fluorescent AGEs (fAGEs). In this preliminary study, we will investigate the effects of glucose, fructose, and galactose, three of the most abundant dietary simple sugars, in fAGEs production. Excised tissues will be treated in solutions containing the three sugar types; multiphoton autofluorescence imaging will then be performed on the treated tissues to determine their autofluroescence levels.
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spelling mit-1721.1/1374742021-11-06T03:00:24Z Multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues Lin, Chih-Ju Kang, Jeon Woong So, Peter T. C. Dong, Chen-Yuan Massachusetts Institute of Technology. Laser Biomedical Research Center Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Department of Biological Engineering © 2020 SPIE. The formation and accumulation of advanced glycation end products (AGEs) contribute to diabetic complications such as retinopathy, neuropathy, nephropathy, and cardiovascular diseases. It is clear that the development of effective technique in AGEs detection and the establishing the correlation of measured AGE parameters to diabetic pathogenesis are invaluable in the monitoring of disease progression and drug discovery of anti-AGE compounds. Since some AGE are fluorescent, we propose to investigate the degree of tissue glycation in forming fluorescent AGEs (fAGEs). In this preliminary study, we will investigate the effects of glucose, fructose, and galactose, three of the most abundant dietary simple sugars, in fAGEs production. Excised tissues will be treated in solutions containing the three sugar types; multiphoton autofluorescence imaging will then be performed on the treated tissues to determine their autofluroescence levels. 2021-11-05T14:19:59Z 2021-11-05T14:19:59Z 2020-02 2020-08-10T15:30:20Z Article http://purl.org/eprint/type/ConferencePaper https://hdl.handle.net/1721.1/137474 Lin, Chih-Ju, Kang, Jeon Woong, So, Peter T. C. and Dong, Chen-Yuan. 2020. "Multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues." Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 11244. en 10.1117/12.2545548 Progress in Biomedical Optics and Imaging - Proceedings of SPIE Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf SPIE SPIE
spellingShingle Lin, Chih-Ju
Kang, Jeon Woong
So, Peter T. C.
Dong, Chen-Yuan
Multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues
title Multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues
title_full Multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues
title_fullStr Multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues
title_full_unstemmed Multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues
title_short Multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues
title_sort multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues
url https://hdl.handle.net/1721.1/137474
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