Microglia enable mature perineuronal nets disassembly upon anesthetic ketamine exposure or 60-Hz light entrainment in the healthy brain
Perineuronal nets (PNNs), components of the extracellular matrix, preferentially coat parvalbumin-positive interneurons and constrain critical-period plasticity in the adult cerebral cortex. Current strategies to remove PNN are long-lasting, invasive, and trigger neuropsychiatric symptoms. Here, we...
| Main Authors: | , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Elsevier BV
2021
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| Online Access: | https://hdl.handle.net/1721.1/138161 |
| _version_ | 1826212991131975680 |
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| author | Venturino, Alessandro Schulz, Rouven De Jesús-Cortés, Héctor Maes, Margaret E Nagy, Bálint Reilly-Andújar, Francis Colombo, Gloria Cubero, Ryan John A Schoot Uiterkamp, Florianne E Bear, Mark F Siegert, Sandra |
| author2 | Picower Institute for Learning and Memory |
| author_facet | Picower Institute for Learning and Memory Venturino, Alessandro Schulz, Rouven De Jesús-Cortés, Héctor Maes, Margaret E Nagy, Bálint Reilly-Andújar, Francis Colombo, Gloria Cubero, Ryan John A Schoot Uiterkamp, Florianne E Bear, Mark F Siegert, Sandra |
| author_sort | Venturino, Alessandro |
| collection | MIT |
| description | Perineuronal nets (PNNs), components of the extracellular matrix, preferentially coat parvalbumin-positive interneurons and constrain critical-period plasticity in the adult cerebral cortex. Current strategies to remove PNN are long-lasting, invasive, and trigger neuropsychiatric symptoms. Here, we apply repeated anesthetic ketamine as a method with minimal behavioral effect. We find that this paradigm strongly reduces PNN coating in the healthy adult brain and promotes juvenile-like plasticity. Microglia are critically involved in PNN loss because they engage with parvalbumin-positive neurons in their defined cortical layer. We identify external 60-Hz light-flickering entrainment to recapitulate microglia-mediated PNN removal. Importantly, 40-Hz frequency, which is known to remove amyloid plaques, does not induce PNN loss, suggesting microglia might functionally tune to distinct brain frequencies. Thus, our 60-Hz light-entrainment strategy provides an alternative form of PNN intervention in the healthy adult brain. |
| first_indexed | 2024-09-23T15:41:21Z |
| format | Article |
| id | mit-1721.1/138161 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T15:41:21Z |
| publishDate | 2021 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1381612023-08-07T18:11:16Z Microglia enable mature perineuronal nets disassembly upon anesthetic ketamine exposure or 60-Hz light entrainment in the healthy brain Venturino, Alessandro Schulz, Rouven De Jesús-Cortés, Héctor Maes, Margaret E Nagy, Bálint Reilly-Andújar, Francis Colombo, Gloria Cubero, Ryan John A Schoot Uiterkamp, Florianne E Bear, Mark F Siegert, Sandra Picower Institute for Learning and Memory Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Perineuronal nets (PNNs), components of the extracellular matrix, preferentially coat parvalbumin-positive interneurons and constrain critical-period plasticity in the adult cerebral cortex. Current strategies to remove PNN are long-lasting, invasive, and trigger neuropsychiatric symptoms. Here, we apply repeated anesthetic ketamine as a method with minimal behavioral effect. We find that this paradigm strongly reduces PNN coating in the healthy adult brain and promotes juvenile-like plasticity. Microglia are critically involved in PNN loss because they engage with parvalbumin-positive neurons in their defined cortical layer. We identify external 60-Hz light-flickering entrainment to recapitulate microglia-mediated PNN removal. Importantly, 40-Hz frequency, which is known to remove amyloid plaques, does not induce PNN loss, suggesting microglia might functionally tune to distinct brain frequencies. Thus, our 60-Hz light-entrainment strategy provides an alternative form of PNN intervention in the healthy adult brain. 2021-11-19T15:25:50Z 2021-11-19T15:25:50Z 2021-07 2021-11-19T15:21:24Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/138161 Venturino, Alessandro, Schulz, Rouven, De Jesús-Cortés, Héctor, Maes, Margaret E, Nagy, Bálint et al. 2021. "Microglia enable mature perineuronal nets disassembly upon anesthetic ketamine exposure or 60-Hz light entrainment in the healthy brain." Cell Reports, 36 (1). en 10.1016/j.celrep.2021.109313 Cell Reports Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Elsevier BV Elsevier |
| spellingShingle | Venturino, Alessandro Schulz, Rouven De Jesús-Cortés, Héctor Maes, Margaret E Nagy, Bálint Reilly-Andújar, Francis Colombo, Gloria Cubero, Ryan John A Schoot Uiterkamp, Florianne E Bear, Mark F Siegert, Sandra Microglia enable mature perineuronal nets disassembly upon anesthetic ketamine exposure or 60-Hz light entrainment in the healthy brain |
| title | Microglia enable mature perineuronal nets disassembly upon anesthetic ketamine exposure or 60-Hz light entrainment in the healthy brain |
| title_full | Microglia enable mature perineuronal nets disassembly upon anesthetic ketamine exposure or 60-Hz light entrainment in the healthy brain |
| title_fullStr | Microglia enable mature perineuronal nets disassembly upon anesthetic ketamine exposure or 60-Hz light entrainment in the healthy brain |
| title_full_unstemmed | Microglia enable mature perineuronal nets disassembly upon anesthetic ketamine exposure or 60-Hz light entrainment in the healthy brain |
| title_short | Microglia enable mature perineuronal nets disassembly upon anesthetic ketamine exposure or 60-Hz light entrainment in the healthy brain |
| title_sort | microglia enable mature perineuronal nets disassembly upon anesthetic ketamine exposure or 60 hz light entrainment in the healthy brain |
| url | https://hdl.handle.net/1721.1/138161 |
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