A genetically encoded near-infrared fluorescent calcium ion indicator
© 2019, The Author(s), under exclusive licence to Springer Nature America, Inc. We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca2+) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively. This GECI, designated NIR-GECO1, enable...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer Nature
2021
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| Online Access: | https://hdl.handle.net/1721.1/138166 |
| _version_ | 1826194695405961216 |
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| author | Qian, Yong Piatkevich, Kiryl D Mc Larney, Benedict Abdelfattah, Ahmed S Mehta, Sohum Murdock, Mitchell H Gottschalk, Sven Molina, Rosana S Zhang, Wei Chen, Yingche Wu, Jiahui Drobizhev, Mikhail Hughes, Thomas E Zhang, Jin Schreiter, Eric R Shoham, Shy Razansky, Daniel Boyden, Edward S Campbell, Robert E |
| author_facet | Qian, Yong Piatkevich, Kiryl D Mc Larney, Benedict Abdelfattah, Ahmed S Mehta, Sohum Murdock, Mitchell H Gottschalk, Sven Molina, Rosana S Zhang, Wei Chen, Yingche Wu, Jiahui Drobizhev, Mikhail Hughes, Thomas E Zhang, Jin Schreiter, Eric R Shoham, Shy Razansky, Daniel Boyden, Edward S Campbell, Robert E |
| author_sort | Qian, Yong |
| collection | MIT |
| description | © 2019, The Author(s), under exclusive licence to Springer Nature America, Inc. We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca2+) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca2+ transients in cultured mammalian cells and brain tissue with sensitivity comparable to that of currently available visible-wavelength GECIs. We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca2+ imaging in combination with other optogenetic indicators and actuators. |
| first_indexed | 2024-09-23T10:00:41Z |
| format | Article |
| id | mit-1721.1/138166 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T10:00:41Z |
| publishDate | 2021 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | mit-1721.1/1381662021-11-20T03:29:51Z A genetically encoded near-infrared fluorescent calcium ion indicator Qian, Yong Piatkevich, Kiryl D Mc Larney, Benedict Abdelfattah, Ahmed S Mehta, Sohum Murdock, Mitchell H Gottschalk, Sven Molina, Rosana S Zhang, Wei Chen, Yingche Wu, Jiahui Drobizhev, Mikhail Hughes, Thomas E Zhang, Jin Schreiter, Eric R Shoham, Shy Razansky, Daniel Boyden, Edward S Campbell, Robert E © 2019, The Author(s), under exclusive licence to Springer Nature America, Inc. We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca2+) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca2+ transients in cultured mammalian cells and brain tissue with sensitivity comparable to that of currently available visible-wavelength GECIs. We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca2+ imaging in combination with other optogenetic indicators and actuators. 2021-11-19T16:19:10Z 2021-11-19T16:19:10Z 2019 2021-11-19T16:15:40Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/138166 Qian, Yong, Piatkevich, Kiryl D, Mc Larney, Benedict, Abdelfattah, Ahmed S, Mehta, Sohum et al. 2019. "A genetically encoded near-infrared fluorescent calcium ion indicator." Nature Methods, 16 (2). en 10.1038/S41592-018-0294-6 Nature Methods Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Springer Nature PMC |
| spellingShingle | Qian, Yong Piatkevich, Kiryl D Mc Larney, Benedict Abdelfattah, Ahmed S Mehta, Sohum Murdock, Mitchell H Gottschalk, Sven Molina, Rosana S Zhang, Wei Chen, Yingche Wu, Jiahui Drobizhev, Mikhail Hughes, Thomas E Zhang, Jin Schreiter, Eric R Shoham, Shy Razansky, Daniel Boyden, Edward S Campbell, Robert E A genetically encoded near-infrared fluorescent calcium ion indicator |
| title | A genetically encoded near-infrared fluorescent calcium ion indicator |
| title_full | A genetically encoded near-infrared fluorescent calcium ion indicator |
| title_fullStr | A genetically encoded near-infrared fluorescent calcium ion indicator |
| title_full_unstemmed | A genetically encoded near-infrared fluorescent calcium ion indicator |
| title_short | A genetically encoded near-infrared fluorescent calcium ion indicator |
| title_sort | genetically encoded near infrared fluorescent calcium ion indicator |
| url | https://hdl.handle.net/1721.1/138166 |
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