Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology
© 2020, The Author(s), under exclusive licence to Springer Nature Limited. In pathology, microscopy is an important tool for the analysis of human tissues, both for the scientific study of disease states and for diagnosis. However, the microscopes commonly used in pathology are limited in resolution...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer Science and Business Media LLC
2021
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| Online Access: | https://hdl.handle.net/1721.1/138168 |
| _version_ | 1811086246938673152 |
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| author | Bucur, Octavian Fu, Feifei Calderon, Mike Mylvaganam, Geetha H Ly, Ngoc L Day, Jimmy Watkin, Simon Walker, Bruce D Boyden, Edward S Zhao, Yongxin |
| author2 | Massachusetts Institute of Technology. Media Laboratory |
| author_facet | Massachusetts Institute of Technology. Media Laboratory Bucur, Octavian Fu, Feifei Calderon, Mike Mylvaganam, Geetha H Ly, Ngoc L Day, Jimmy Watkin, Simon Walker, Bruce D Boyden, Edward S Zhao, Yongxin |
| author_sort | Bucur, Octavian |
| collection | MIT |
| description | © 2020, The Author(s), under exclusive licence to Springer Nature Limited. In pathology, microscopy is an important tool for the analysis of human tissues, both for the scientific study of disease states and for diagnosis. However, the microscopes commonly used in pathology are limited in resolution by diffraction. Recently, we discovered that it was possible, through a chemical process, to isotropically expand preserved cells and tissues by 4–5× in linear dimension. We call this process expansion microscopy (ExM). ExM enables nanoscale resolution imaging on conventional microscopes. Here we describe protocols for the simple and effective physical expansion of a variety of human tissues and clinical specimens, including paraffin-embedded, fresh frozen and chemically stained human tissues. These protocols require only inexpensive, commercially available reagents and hardware commonly found in a routine pathology laboratory. Our protocols are written for researchers and pathologists experienced in conventional fluorescence microscopy. The conventional protocol, expansion pathology, can be completed in ~1 d with immunostained tissue sections and 2 d with unstained specimens. We also include a new, fast variant, rapid expansion pathology, that can be performed on <5-µm-thick tissue sections, taking <4 h with immunostained tissue sections and <8 h with unstained specimens. |
| first_indexed | 2024-09-23T13:23:12Z |
| format | Article |
| id | mit-1721.1/138168 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T13:23:12Z |
| publishDate | 2021 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1381682024-03-19T13:56:28Z Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology Bucur, Octavian Fu, Feifei Calderon, Mike Mylvaganam, Geetha H Ly, Ngoc L Day, Jimmy Watkin, Simon Walker, Bruce D Boyden, Edward S Zhao, Yongxin Massachusetts Institute of Technology. Media Laboratory Massachusetts Institute of Technology. Institute for Medical Engineering & Science Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Massachusetts Institute of Technology. Department of Biological Engineering McGovern Institute for Brain Research at MIT Koch Institute for Integrative Cancer Research at MIT Massachusetts Institute of Technology. Center for Neurobiological Engineering © 2020, The Author(s), under exclusive licence to Springer Nature Limited. In pathology, microscopy is an important tool for the analysis of human tissues, both for the scientific study of disease states and for diagnosis. However, the microscopes commonly used in pathology are limited in resolution by diffraction. Recently, we discovered that it was possible, through a chemical process, to isotropically expand preserved cells and tissues by 4–5× in linear dimension. We call this process expansion microscopy (ExM). ExM enables nanoscale resolution imaging on conventional microscopes. Here we describe protocols for the simple and effective physical expansion of a variety of human tissues and clinical specimens, including paraffin-embedded, fresh frozen and chemically stained human tissues. These protocols require only inexpensive, commercially available reagents and hardware commonly found in a routine pathology laboratory. Our protocols are written for researchers and pathologists experienced in conventional fluorescence microscopy. The conventional protocol, expansion pathology, can be completed in ~1 d with immunostained tissue sections and 2 d with unstained specimens. We also include a new, fast variant, rapid expansion pathology, that can be performed on <5-µm-thick tissue sections, taking <4 h with immunostained tissue sections and <8 h with unstained specimens. 2021-11-19T19:19:35Z 2021-11-19T19:19:35Z 2020 2021-11-19T19:13:28Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/138168 Bucur, Octavian, Fu, Feifei, Calderon, Mike, Mylvaganam, Geetha H, Ly, Ngoc L et al. 2020. "Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology." Nature Protocols, 15 (5). en 10.1038/S41596-020-0300-1 Nature Protocols Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Springer Science and Business Media LLC PMC |
| spellingShingle | Bucur, Octavian Fu, Feifei Calderon, Mike Mylvaganam, Geetha H Ly, Ngoc L Day, Jimmy Watkin, Simon Walker, Bruce D Boyden, Edward S Zhao, Yongxin Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology |
| title | Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology |
| title_full | Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology |
| title_fullStr | Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology |
| title_full_unstemmed | Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology |
| title_short | Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology |
| title_sort | nanoscale imaging of clinical specimens using conventional and rapid expansion pathology |
| url | https://hdl.handle.net/1721.1/138168 |
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