Chronically implantable LED arrays for behavioral optogenetics in primates
Optogenetic methods have been widely used in rodent brains, but remain relatively under-developed for nonhuman primates such as rhesus macaques, an animal model with a large brain expressing sophisticated sensory, motor and cognitive behaviors. To address challenges in behavioral optogenetics in lar...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
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Springer Science and Business Media LLC
2021
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| Online Access: | https://hdl.handle.net/1721.1/138202 |
| _version_ | 1826209252560076800 |
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| author | Rajalingham, Rishi Sorenson, Michael Azadi, Reza Bohn, Simon DiCarlo, James J Afraz, Arash |
| author2 | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences |
| author_facet | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Rajalingham, Rishi Sorenson, Michael Azadi, Reza Bohn, Simon DiCarlo, James J Afraz, Arash |
| author_sort | Rajalingham, Rishi |
| collection | MIT |
| description | Optogenetic methods have been widely used in rodent brains, but remain relatively under-developed for nonhuman primates such as rhesus macaques, an animal model with a large brain expressing sophisticated sensory, motor and cognitive behaviors. To address challenges in behavioral optogenetics in large brains, we developed Opto-Array, a chronically implantable array of light-emitting diodes for high-throughput optogenetic perturbation. We demonstrated that optogenetic silencing in the macaque primary visual cortex with the help of the Opto-Array results in reliable retinotopic visual deficits in a luminance discrimination task. We separately confirmed that Opto-Array illumination results in local neural silencing, and that behavioral effects are not due to tissue heating. These results demonstrate the effectiveness of the Opto-Array for behavioral optogenetic applications in large brains. |
| first_indexed | 2024-09-23T14:19:35Z |
| format | Article |
| id | mit-1721.1/138202 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T14:19:35Z |
| publishDate | 2021 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1382022023-04-13T16:04:41Z Chronically implantable LED arrays for behavioral optogenetics in primates Rajalingham, Rishi Sorenson, Michael Azadi, Reza Bohn, Simon DiCarlo, James J Afraz, Arash Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences McGovern Institute for Brain Research at MIT Center for Brains, Minds, and Machines Optogenetic methods have been widely used in rodent brains, but remain relatively under-developed for nonhuman primates such as rhesus macaques, an animal model with a large brain expressing sophisticated sensory, motor and cognitive behaviors. To address challenges in behavioral optogenetics in large brains, we developed Opto-Array, a chronically implantable array of light-emitting diodes for high-throughput optogenetic perturbation. We demonstrated that optogenetic silencing in the macaque primary visual cortex with the help of the Opto-Array results in reliable retinotopic visual deficits in a luminance discrimination task. We separately confirmed that Opto-Array illumination results in local neural silencing, and that behavioral effects are not due to tissue heating. These results demonstrate the effectiveness of the Opto-Array for behavioral optogenetic applications in large brains. 2021-11-22T19:47:49Z 2021-11-22T19:47:49Z 2021-09 2021-11-22T19:43:38Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/138202 Rajalingham, Rishi, Sorenson, Michael, Azadi, Reza, Bohn, Simon, DiCarlo, James J et al. 2021. "Chronically implantable LED arrays for behavioral optogenetics in primates." Nature Methods, 18 (9). en 10.1038/s41592-021-01238-9 Nature Methods Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Springer Science and Business Media LLC bioRxiv |
| spellingShingle | Rajalingham, Rishi Sorenson, Michael Azadi, Reza Bohn, Simon DiCarlo, James J Afraz, Arash Chronically implantable LED arrays for behavioral optogenetics in primates |
| title | Chronically implantable LED arrays for behavioral optogenetics in primates |
| title_full | Chronically implantable LED arrays for behavioral optogenetics in primates |
| title_fullStr | Chronically implantable LED arrays for behavioral optogenetics in primates |
| title_full_unstemmed | Chronically implantable LED arrays for behavioral optogenetics in primates |
| title_short | Chronically implantable LED arrays for behavioral optogenetics in primates |
| title_sort | chronically implantable led arrays for behavioral optogenetics in primates |
| url | https://hdl.handle.net/1721.1/138202 |
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