Widespread and Highly Correlated Somato-dendritic Activity in Cortical Layer 5 Neurons
© 2019 Elsevier Inc. Dendritic integration can expand the information-processing capabilities of neurons. However, the recruitment of active dendritic processing in vivo and its relationship to somatic activity remain poorly understood. Here, we use two-photon GCaMP6f imaging to simultaneously monit...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier BV
2021
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| Online Access: | https://hdl.handle.net/1721.1/138264 |
| _version_ | 1811097408021463040 |
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| author | Beaulieu-Laroche, Lou Toloza, Enrique HS Brown, Norma J Harnett, Mark T |
| author_facet | Beaulieu-Laroche, Lou Toloza, Enrique HS Brown, Norma J Harnett, Mark T |
| author_sort | Beaulieu-Laroche, Lou |
| collection | MIT |
| description | © 2019 Elsevier Inc. Dendritic integration can expand the information-processing capabilities of neurons. However, the recruitment of active dendritic processing in vivo and its relationship to somatic activity remain poorly understood. Here, we use two-photon GCaMP6f imaging to simultaneously monitor dendritic and somatic compartments in the awake primary visual cortex. Activity in layer 5 pyramidal neuron somata and distal apical trunk dendrites shows surprisingly high functional correlation. This strong coupling persists across neural activity levels and is unchanged by visual stimuli and locomotion. Ex vivo combined somato-dendritic patch-clamp and GCaMP6f recordings indicate that dendritic signals specifically reflect local electrogenesis triggered by dendritic inputs or high-frequency bursts of somatic action potentials. In contrast to the view that dendrites are only sparsely recruited under highly specific conditions in vivo, our results provide evidence that active dendritic integration is a widespread and intrinsic feature of cortical computation. Beaulieu-Laroche et al. perform near-simultaneous calcium imaging of somatic and dendritic activity to reveal that active dendritic integration is an integral feature of information processing in cortical pyramidal neurons. |
| first_indexed | 2024-09-23T16:59:03Z |
| format | Article |
| id | mit-1721.1/138264 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T16:59:03Z |
| publishDate | 2021 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1382642021-12-02T03:01:02Z Widespread and Highly Correlated Somato-dendritic Activity in Cortical Layer 5 Neurons Beaulieu-Laroche, Lou Toloza, Enrique HS Brown, Norma J Harnett, Mark T © 2019 Elsevier Inc. Dendritic integration can expand the information-processing capabilities of neurons. However, the recruitment of active dendritic processing in vivo and its relationship to somatic activity remain poorly understood. Here, we use two-photon GCaMP6f imaging to simultaneously monitor dendritic and somatic compartments in the awake primary visual cortex. Activity in layer 5 pyramidal neuron somata and distal apical trunk dendrites shows surprisingly high functional correlation. This strong coupling persists across neural activity levels and is unchanged by visual stimuli and locomotion. Ex vivo combined somato-dendritic patch-clamp and GCaMP6f recordings indicate that dendritic signals specifically reflect local electrogenesis triggered by dendritic inputs or high-frequency bursts of somatic action potentials. In contrast to the view that dendrites are only sparsely recruited under highly specific conditions in vivo, our results provide evidence that active dendritic integration is a widespread and intrinsic feature of cortical computation. Beaulieu-Laroche et al. perform near-simultaneous calcium imaging of somatic and dendritic activity to reveal that active dendritic integration is an integral feature of information processing in cortical pyramidal neurons. 2021-12-01T15:17:05Z 2021-12-01T15:17:05Z 2019 2021-12-01T15:13:59Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/138264 Beaulieu-Laroche, Lou, Toloza, Enrique HS, Brown, Norma J and Harnett, Mark T. 2019. "Widespread and Highly Correlated Somato-dendritic Activity in Cortical Layer 5 Neurons." Neuron, 103 (2). en 10.1016/J.NEURON.2019.05.014 Neuron Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier BV PMC |
| spellingShingle | Beaulieu-Laroche, Lou Toloza, Enrique HS Brown, Norma J Harnett, Mark T Widespread and Highly Correlated Somato-dendritic Activity in Cortical Layer 5 Neurons |
| title | Widespread and Highly Correlated Somato-dendritic Activity in Cortical Layer 5 Neurons |
| title_full | Widespread and Highly Correlated Somato-dendritic Activity in Cortical Layer 5 Neurons |
| title_fullStr | Widespread and Highly Correlated Somato-dendritic Activity in Cortical Layer 5 Neurons |
| title_full_unstemmed | Widespread and Highly Correlated Somato-dendritic Activity in Cortical Layer 5 Neurons |
| title_short | Widespread and Highly Correlated Somato-dendritic Activity in Cortical Layer 5 Neurons |
| title_sort | widespread and highly correlated somato dendritic activity in cortical layer 5 neurons |
| url | https://hdl.handle.net/1721.1/138264 |
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