Transcriptional and imprinting complexity in Arabidopsis seeds at single-nucleus resolution
Seeds are a key life cycle stage for many plants. Seeds are also the basis of agriculture and the primary source of calories consumed by humans1. Here, we employ single-nucleus RNA-sequencing to generate a transcriptional atlas of developing Arabidopsis thaliana seeds, with a focus on endosperm. End...
| Main Authors: | , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Springer Science and Business Media LLC
2021
|
| Online Access: | https://hdl.handle.net/1721.1/138287 |
| _version_ | 1826198262116253696 |
|---|---|
| author | Picard, Colette L Povilus, Rebecca A Williams, Ben P Gehring, Mary |
| author2 | Whitehead Institute for Biomedical Research |
| author_facet | Whitehead Institute for Biomedical Research Picard, Colette L Povilus, Rebecca A Williams, Ben P Gehring, Mary |
| author_sort | Picard, Colette L |
| collection | MIT |
| description | Seeds are a key life cycle stage for many plants. Seeds are also the basis of agriculture and the primary source of calories consumed by humans1. Here, we employ single-nucleus RNA-sequencing to generate a transcriptional atlas of developing Arabidopsis thaliana seeds, with a focus on endosperm. Endosperm, the primary site of gene imprinting in flowering plants, mediates the relationship between the maternal parent and the embryo2. We identify transcriptionally uncharacterized nuclei types in the chalazal endosperm, which interfaces with maternal tissue for nutrient unloading3,4. We demonstrate that the extent of parental bias of maternally expressed imprinted genes varies with cell-cycle phase, and that imprinting of paternally expressed imprinted genes is strongest in chalazal endosperm. Thus, imprinting is spatially and temporally heterogeneous. Increased paternal expression in the chalazal region suggests that parental conflict, which is proposed to drive imprinting evolution, is fiercest at the boundary between filial and maternal tissues. |
| first_indexed | 2024-09-23T11:02:02Z |
| format | Article |
| id | mit-1721.1/138287 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T11:02:02Z |
| publishDate | 2021 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1382872023-04-14T19:49:52Z Transcriptional and imprinting complexity in Arabidopsis seeds at single-nucleus resolution Picard, Colette L Povilus, Rebecca A Williams, Ben P Gehring, Mary Whitehead Institute for Biomedical Research Massachusetts Institute of Technology. Computational and Systems Biology Program Massachusetts Institute of Technology. Department of Biology Seeds are a key life cycle stage for many plants. Seeds are also the basis of agriculture and the primary source of calories consumed by humans1. Here, we employ single-nucleus RNA-sequencing to generate a transcriptional atlas of developing Arabidopsis thaliana seeds, with a focus on endosperm. Endosperm, the primary site of gene imprinting in flowering plants, mediates the relationship between the maternal parent and the embryo2. We identify transcriptionally uncharacterized nuclei types in the chalazal endosperm, which interfaces with maternal tissue for nutrient unloading3,4. We demonstrate that the extent of parental bias of maternally expressed imprinted genes varies with cell-cycle phase, and that imprinting of paternally expressed imprinted genes is strongest in chalazal endosperm. Thus, imprinting is spatially and temporally heterogeneous. Increased paternal expression in the chalazal region suggests that parental conflict, which is proposed to drive imprinting evolution, is fiercest at the boundary between filial and maternal tissues. 2021-12-01T19:33:38Z 2021-12-01T19:33:38Z 2021 2021-12-01T19:32:00Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/138287 Picard, Colette L, Povilus, Rebecca A, Williams, Ben P and Gehring, Mary. 2021. "Transcriptional and imprinting complexity in Arabidopsis seeds at single-nucleus resolution." Nature Plants, 7 (6). en 10.1038/S41477-021-00922-0 Nature Plants Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Springer Science and Business Media LLC PMC |
| spellingShingle | Picard, Colette L Povilus, Rebecca A Williams, Ben P Gehring, Mary Transcriptional and imprinting complexity in Arabidopsis seeds at single-nucleus resolution |
| title | Transcriptional and imprinting complexity in Arabidopsis seeds at single-nucleus resolution |
| title_full | Transcriptional and imprinting complexity in Arabidopsis seeds at single-nucleus resolution |
| title_fullStr | Transcriptional and imprinting complexity in Arabidopsis seeds at single-nucleus resolution |
| title_full_unstemmed | Transcriptional and imprinting complexity in Arabidopsis seeds at single-nucleus resolution |
| title_short | Transcriptional and imprinting complexity in Arabidopsis seeds at single-nucleus resolution |
| title_sort | transcriptional and imprinting complexity in arabidopsis seeds at single nucleus resolution |
| url | https://hdl.handle.net/1721.1/138287 |
| work_keys_str_mv | AT picardcolettel transcriptionalandimprintingcomplexityinarabidopsisseedsatsinglenucleusresolution AT povilusrebeccaa transcriptionalandimprintingcomplexityinarabidopsisseedsatsinglenucleusresolution AT williamsbenp transcriptionalandimprintingcomplexityinarabidopsisseedsatsinglenucleusresolution AT gehringmary transcriptionalandimprintingcomplexityinarabidopsisseedsatsinglenucleusresolution |