Mechanisms Driving Neutrophil-Induced T-cell Immunoparalysis in Ovarian Cancer
T-cell activation and expansion in the tumor microenvironment (TME) are critical for antitumor immunity. Neutrophils in the TME acquire a complement-dependent T-cell suppressor phenotype that is characterized by inhibition of T-cell proliferation and activation through mechanisms distinct from those...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Association for Cancer Research (AACR)
2021
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| Online Access: | https://hdl.handle.net/1721.1/138289 |
| _version_ | 1811081598774280192 |
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| author | Emmons, Tiffany R Giridharan, Thejaswini Singel, Kelly L Khan, ANM Nazmul H Ricciuti, Jason Howard, Kaitlyn Silva-Del Toro, Stephanie L Debreceni, Ivy L Aarts, Cathelijn EM Brouwer, Mieke C Suzuki, Sora Kuijpers, Taco W Jongerius, Ilse Allen, Lee-Ann H Ferreira, Viviana P Schubart, Anna Sellner, Holger Eder, Jörg Holland, Steven M Ram, Sanjay Lederer, James A Eng, Kevin H Moysich, Kirsten B Odunsi, Kunle Yaffe, Michael B Zsiros, Emese Segal, Brahm H |
| author_facet | Emmons, Tiffany R Giridharan, Thejaswini Singel, Kelly L Khan, ANM Nazmul H Ricciuti, Jason Howard, Kaitlyn Silva-Del Toro, Stephanie L Debreceni, Ivy L Aarts, Cathelijn EM Brouwer, Mieke C Suzuki, Sora Kuijpers, Taco W Jongerius, Ilse Allen, Lee-Ann H Ferreira, Viviana P Schubart, Anna Sellner, Holger Eder, Jörg Holland, Steven M Ram, Sanjay Lederer, James A Eng, Kevin H Moysich, Kirsten B Odunsi, Kunle Yaffe, Michael B Zsiros, Emese Segal, Brahm H |
| author_sort | Emmons, Tiffany R |
| collection | MIT |
| description | T-cell activation and expansion in the tumor microenvironment (TME) are critical for antitumor immunity. Neutrophils in the TME acquire a complement-dependent T-cell suppressor phenotype that is characterized by inhibition of T-cell proliferation and activation through mechanisms distinct from those of myeloid-derived suppressor cells. In this study, we used ascites fluid supernatants (ASC) from patients with ovarian cancer as an authentic component of the TME to evaluate the effects of ASC on neutrophil function and mechanisms for neutrophil-driven immune suppression. ASC prolonged neutrophil life span, decreased neutrophil density, and induced nuclear hypersegmentation. Mass cytometry analysis showed that ASC induced 15 distinct neutrophil clusters. ASC stimulated complement deposition and signaling in neutrophils, resulting in surface mobilization of granule constituents, including NADPH oxidase. NADPH oxidase activation and phosphatidylserine signaling were required for neutrophil suppressor function, although we did not observe a direct role of extracellular reactive oxygen species in inhibiting T-cell proliferation. Postoperative surgical drainage fluid also induced a complement-dependent neutrophil suppressor phenotype, pointing to this effect as a general response to injury. Like circulating lymphocytes, ASC-activated neutrophils caused complement-dependent suppression of tumor-associated lymphocytes. ASC-activated neutrophils adhered to T cells and caused trogocytosis of T-cell membranes. These injury and signaling cues resulted in T-cell immunoparalysis characterized by impaired NFAT translocation, IL2 production, glucose uptake, mitochondrial function, and mTOR activation. Our results demonstrate that complement-dependent priming of neutrophil effector functions in the TME induces a T-cell nonresponsiveness distinct from established checkpoint pathways and identify targets for immunotherapy.See related Spotlight by Cassatella, p. 725. |
| first_indexed | 2024-09-23T11:49:20Z |
| format | Article |
| id | mit-1721.1/138289 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T11:49:20Z |
| publishDate | 2021 |
| publisher | American Association for Cancer Research (AACR) |
| record_format | dspace |
| spelling | mit-1721.1/1382892021-12-03T03:22:33Z Mechanisms Driving Neutrophil-Induced T-cell Immunoparalysis in Ovarian Cancer Emmons, Tiffany R Giridharan, Thejaswini Singel, Kelly L Khan, ANM Nazmul H Ricciuti, Jason Howard, Kaitlyn Silva-Del Toro, Stephanie L Debreceni, Ivy L Aarts, Cathelijn EM Brouwer, Mieke C Suzuki, Sora Kuijpers, Taco W Jongerius, Ilse Allen, Lee-Ann H Ferreira, Viviana P Schubart, Anna Sellner, Holger Eder, Jörg Holland, Steven M Ram, Sanjay Lederer, James A Eng, Kevin H Moysich, Kirsten B Odunsi, Kunle Yaffe, Michael B Zsiros, Emese Segal, Brahm H T-cell activation and expansion in the tumor microenvironment (TME) are critical for antitumor immunity. Neutrophils in the TME acquire a complement-dependent T-cell suppressor phenotype that is characterized by inhibition of T-cell proliferation and activation through mechanisms distinct from those of myeloid-derived suppressor cells. In this study, we used ascites fluid supernatants (ASC) from patients with ovarian cancer as an authentic component of the TME to evaluate the effects of ASC on neutrophil function and mechanisms for neutrophil-driven immune suppression. ASC prolonged neutrophil life span, decreased neutrophil density, and induced nuclear hypersegmentation. Mass cytometry analysis showed that ASC induced 15 distinct neutrophil clusters. ASC stimulated complement deposition and signaling in neutrophils, resulting in surface mobilization of granule constituents, including NADPH oxidase. NADPH oxidase activation and phosphatidylserine signaling were required for neutrophil suppressor function, although we did not observe a direct role of extracellular reactive oxygen species in inhibiting T-cell proliferation. Postoperative surgical drainage fluid also induced a complement-dependent neutrophil suppressor phenotype, pointing to this effect as a general response to injury. Like circulating lymphocytes, ASC-activated neutrophils caused complement-dependent suppression of tumor-associated lymphocytes. ASC-activated neutrophils adhered to T cells and caused trogocytosis of T-cell membranes. These injury and signaling cues resulted in T-cell immunoparalysis characterized by impaired NFAT translocation, IL2 production, glucose uptake, mitochondrial function, and mTOR activation. Our results demonstrate that complement-dependent priming of neutrophil effector functions in the TME induces a T-cell nonresponsiveness distinct from established checkpoint pathways and identify targets for immunotherapy.See related Spotlight by Cassatella, p. 725. 2021-12-02T14:40:43Z 2021-12-02T14:40:43Z 2021-07 2021-12-02T14:27:59Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/138289 Emmons, Tiffany R, Giridharan, Thejaswini, Singel, Kelly L, Khan, ANM Nazmul H, Ricciuti, Jason et al. 2021. "Mechanisms Driving Neutrophil-Induced T-cell Immunoparalysis in Ovarian Cancer." Cancer Immunology Research, 9 (7). en 10.1158/2326-6066.cir-20-0922 Cancer Immunology Research Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf American Association for Cancer Research (AACR) PMC |
| spellingShingle | Emmons, Tiffany R Giridharan, Thejaswini Singel, Kelly L Khan, ANM Nazmul H Ricciuti, Jason Howard, Kaitlyn Silva-Del Toro, Stephanie L Debreceni, Ivy L Aarts, Cathelijn EM Brouwer, Mieke C Suzuki, Sora Kuijpers, Taco W Jongerius, Ilse Allen, Lee-Ann H Ferreira, Viviana P Schubart, Anna Sellner, Holger Eder, Jörg Holland, Steven M Ram, Sanjay Lederer, James A Eng, Kevin H Moysich, Kirsten B Odunsi, Kunle Yaffe, Michael B Zsiros, Emese Segal, Brahm H Mechanisms Driving Neutrophil-Induced T-cell Immunoparalysis in Ovarian Cancer |
| title | Mechanisms Driving Neutrophil-Induced T-cell Immunoparalysis in Ovarian Cancer |
| title_full | Mechanisms Driving Neutrophil-Induced T-cell Immunoparalysis in Ovarian Cancer |
| title_fullStr | Mechanisms Driving Neutrophil-Induced T-cell Immunoparalysis in Ovarian Cancer |
| title_full_unstemmed | Mechanisms Driving Neutrophil-Induced T-cell Immunoparalysis in Ovarian Cancer |
| title_short | Mechanisms Driving Neutrophil-Induced T-cell Immunoparalysis in Ovarian Cancer |
| title_sort | mechanisms driving neutrophil induced t cell immunoparalysis in ovarian cancer |
| url | https://hdl.handle.net/1721.1/138289 |
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