De-scattering with Excitation Patterning enables rapid wide-field imaging through scattering media
Nonlinear optical microscopy has enabled in vivo deep tissue imaging on the millimeter scale. A key unmet challenge is its limited throughput especially compared to rapid wide-field modalities that are used ubiquitously in thin specimens. Wide-field imaging methods in tissue specimens have found suc...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Association for the Advancement of Science (AAAS)
2021
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| Online Access: | https://hdl.handle.net/1721.1/138331 |
| _version_ | 1826188045401980928 |
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| author | Zheng, Cheng Park, Jong Kang Yildirim, Murat Boivin, Josiah R Xue, Yi Sur, Mriganka So, Peter TC Wadduwage, Dushan N |
| author2 | Massachusetts Institute of Technology. Department of Mechanical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Mechanical Engineering Zheng, Cheng Park, Jong Kang Yildirim, Murat Boivin, Josiah R Xue, Yi Sur, Mriganka So, Peter TC Wadduwage, Dushan N |
| author_sort | Zheng, Cheng |
| collection | MIT |
| description | Nonlinear optical microscopy has enabled in vivo deep tissue imaging on the millimeter scale. A key unmet challenge is its limited throughput especially compared to rapid wide-field modalities that are used ubiquitously in thin specimens. Wide-field imaging methods in tissue specimens have found successes in optically cleared tissues and at shallower depths, but the scattering of emission photons in thick turbid samples severely degrades image quality at the camera. To address this challenge, we introduce a novel technique called De-scattering with Excitation Patterning or "DEEP," which uses patterned nonlinear excitation followed by computational imaging-assisted wide-field detection. Multiphoton temporal focusing allows high-resolution excitation patterns to be projected deep inside specimen at multiple scattering lengths due to the use of long wavelength light. Computational reconstruction allows high-resolution structural features to be reconstructed from tens to hundreds of DEEP images instead of millions of point-scanning measurements. |
| first_indexed | 2024-09-23T07:53:45Z |
| format | Article |
| id | mit-1721.1/138331 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T07:53:45Z |
| publishDate | 2021 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | dspace |
| spelling | mit-1721.1/1383312023-04-18T18:23:09Z De-scattering with Excitation Patterning enables rapid wide-field imaging through scattering media Zheng, Cheng Park, Jong Kang Yildirim, Murat Boivin, Josiah R Xue, Yi Sur, Mriganka So, Peter TC Wadduwage, Dushan N Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Laser Biomedical Research Center Massachusetts Institute of Technology. Department of Biological Engineering Picower Institute for Learning and Memory Nonlinear optical microscopy has enabled in vivo deep tissue imaging on the millimeter scale. A key unmet challenge is its limited throughput especially compared to rapid wide-field modalities that are used ubiquitously in thin specimens. Wide-field imaging methods in tissue specimens have found successes in optically cleared tissues and at shallower depths, but the scattering of emission photons in thick turbid samples severely degrades image quality at the camera. To address this challenge, we introduce a novel technique called De-scattering with Excitation Patterning or "DEEP," which uses patterned nonlinear excitation followed by computational imaging-assisted wide-field detection. Multiphoton temporal focusing allows high-resolution excitation patterns to be projected deep inside specimen at multiple scattering lengths due to the use of long wavelength light. Computational reconstruction allows high-resolution structural features to be reconstructed from tens to hundreds of DEEP images instead of millions of point-scanning measurements. 2021-12-06T17:58:08Z 2021-12-06T17:58:08Z 2021-07 2021-12-06T17:54:06Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/138331 Zheng, Cheng, Park, Jong Kang, Yildirim, Murat, Boivin, Josiah R, Xue, Yi et al. 2021. "De-scattering with Excitation Patterning enables rapid wide-field imaging through scattering media." Science Advances, 7 (28). en 10.1126/sciadv.aay5496 Science Advances Creative Commons Attribution NonCommercial License 4.0 https://creativecommons.org/licenses/by-nc/4.0/ application/pdf American Association for the Advancement of Science (AAAS) Science Advances |
| spellingShingle | Zheng, Cheng Park, Jong Kang Yildirim, Murat Boivin, Josiah R Xue, Yi Sur, Mriganka So, Peter TC Wadduwage, Dushan N De-scattering with Excitation Patterning enables rapid wide-field imaging through scattering media |
| title | De-scattering with Excitation Patterning enables rapid wide-field imaging through scattering media |
| title_full | De-scattering with Excitation Patterning enables rapid wide-field imaging through scattering media |
| title_fullStr | De-scattering with Excitation Patterning enables rapid wide-field imaging through scattering media |
| title_full_unstemmed | De-scattering with Excitation Patterning enables rapid wide-field imaging through scattering media |
| title_short | De-scattering with Excitation Patterning enables rapid wide-field imaging through scattering media |
| title_sort | de scattering with excitation patterning enables rapid wide field imaging through scattering media |
| url | https://hdl.handle.net/1721.1/138331 |
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