Constructing an adult orofacial premotor atlas in Allen mouse CCF
Premotor circuits in the brainstem project to pools of orofacial motoneurons to execute essential motor action such as licking, chewing, breathing, and in rodent, whisking. Previous transsynaptic tracing studies only mapped orofacial premotor circuits in neonatal mice, but the adult circuits remain...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications, Ltd
2021
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| Online Access: | https://hdl.handle.net/1721.1/138338 |
| _version_ | 1826212374220111872 |
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| author | Takatoh, Jun Park, Jae Hong Lu, Jinghao Li, Shun Thompson, PM Han, Bao-Xia Zhao, Shengli Kleinfeld, David Friedman, Beth Wang, Fan |
| author_facet | Takatoh, Jun Park, Jae Hong Lu, Jinghao Li, Shun Thompson, PM Han, Bao-Xia Zhao, Shengli Kleinfeld, David Friedman, Beth Wang, Fan |
| author_sort | Takatoh, Jun |
| collection | MIT |
| description | Premotor circuits in the brainstem project to pools of orofacial motoneurons to execute essential motor action such as licking, chewing, breathing, and in rodent, whisking. Previous transsynaptic tracing studies only mapped orofacial premotor circuits in neonatal mice, but the adult circuits remain unknown as a consequence of technical difficulties. Here, we developed a three-step monosynaptic transsynaptic tracing strategy to identify premotor neurons controlling vibrissa, tongue protrusion, and jaw-closing muscles in the adult mouse. We registered these different groups of premotor neurons onto the Allen mouse brain common coordinate framework (CCF) and consequently generated a combined 3D orofacial premotor atlas, revealing unique spatial organizations of distinct premotor circuits. We further uncovered premotor neurons that simultaneously innervate multiple motor nuclei and, consequently, are likely to coordinate different muscles involved in the same orofacial motor actions. Our method for tracing adult premotor circuits and registering to Allen CCF is generally applicable and should facilitate the investigations of motor controls of diverse behaviors. |
| first_indexed | 2024-09-23T15:20:40Z |
| format | Article |
| id | mit-1721.1/138338 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T15:20:40Z |
| publishDate | 2021 |
| publisher | eLife Sciences Publications, Ltd |
| record_format | dspace |
| spelling | mit-1721.1/1383382021-12-08T03:05:22Z Constructing an adult orofacial premotor atlas in Allen mouse CCF Takatoh, Jun Park, Jae Hong Lu, Jinghao Li, Shun Thompson, PM Han, Bao-Xia Zhao, Shengli Kleinfeld, David Friedman, Beth Wang, Fan Premotor circuits in the brainstem project to pools of orofacial motoneurons to execute essential motor action such as licking, chewing, breathing, and in rodent, whisking. Previous transsynaptic tracing studies only mapped orofacial premotor circuits in neonatal mice, but the adult circuits remain unknown as a consequence of technical difficulties. Here, we developed a three-step monosynaptic transsynaptic tracing strategy to identify premotor neurons controlling vibrissa, tongue protrusion, and jaw-closing muscles in the adult mouse. We registered these different groups of premotor neurons onto the Allen mouse brain common coordinate framework (CCF) and consequently generated a combined 3D orofacial premotor atlas, revealing unique spatial organizations of distinct premotor circuits. We further uncovered premotor neurons that simultaneously innervate multiple motor nuclei and, consequently, are likely to coordinate different muscles involved in the same orofacial motor actions. Our method for tracing adult premotor circuits and registering to Allen CCF is generally applicable and should facilitate the investigations of motor controls of diverse behaviors. 2021-12-07T12:57:31Z 2021-12-07T12:57:31Z 2021 2021-12-07T12:51:54Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/138338 Takatoh, Jun, Park, Jae Hong, Lu, Jinghao, Li, Shun, Thompson, PM et al. 2021. "Constructing an adult orofacial premotor atlas in Allen mouse CCF." eLife, 10. en 10.7554/ELIFE.67291 eLife Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf eLife Sciences Publications, Ltd eLife |
| spellingShingle | Takatoh, Jun Park, Jae Hong Lu, Jinghao Li, Shun Thompson, PM Han, Bao-Xia Zhao, Shengli Kleinfeld, David Friedman, Beth Wang, Fan Constructing an adult orofacial premotor atlas in Allen mouse CCF |
| title | Constructing an adult orofacial premotor atlas in Allen mouse CCF |
| title_full | Constructing an adult orofacial premotor atlas in Allen mouse CCF |
| title_fullStr | Constructing an adult orofacial premotor atlas in Allen mouse CCF |
| title_full_unstemmed | Constructing an adult orofacial premotor atlas in Allen mouse CCF |
| title_short | Constructing an adult orofacial premotor atlas in Allen mouse CCF |
| title_sort | constructing an adult orofacial premotor atlas in allen mouse ccf |
| url | https://hdl.handle.net/1721.1/138338 |
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