Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13
Nucleic acid detection is vital for agricultural applications including trait detection during breeding, pest surveillance, and pathogen identification. Here, we use a modified version of the CRISPR-based nucleic acid detection platform SHERLOCK to quantify levels of a glyphosate resistance gene in...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Mary Ann Liebert Inc
2021
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| Online Access: | https://hdl.handle.net/1721.1/138384 |
| _version_ | 1811095513734316032 |
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| author | Abudayyeh, Omar O Gootenberg, Jonathan S Kellner, Max J Zhang, Feng |
| author_facet | Abudayyeh, Omar O Gootenberg, Jonathan S Kellner, Max J Zhang, Feng |
| author_sort | Abudayyeh, Omar O |
| collection | MIT |
| description | Nucleic acid detection is vital for agricultural applications including trait detection during breeding, pest surveillance, and pathogen identification. Here, we use a modified version of the CRISPR-based nucleic acid detection platform SHERLOCK to quantify levels of a glyphosate resistance gene in a mixture of soybeans and to detect multiple plant genes in a single reaction. SHERLOCK is rapid (∼15 min), quantitative, and portable, and can process crude soybean extracts as input material for minimal nucleic acid sample preparation. This field-ready SHERLOCK platform with color-based lateral flow readout can be applied for detection and quantitation of genes in a range of agricultural applications. |
| first_indexed | 2024-09-23T16:18:14Z |
| format | Article |
| id | mit-1721.1/138384 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T16:18:14Z |
| publishDate | 2021 |
| publisher | Mary Ann Liebert Inc |
| record_format | dspace |
| spelling | mit-1721.1/1383842021-12-09T03:02:00Z Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13 Abudayyeh, Omar O Gootenberg, Jonathan S Kellner, Max J Zhang, Feng Nucleic acid detection is vital for agricultural applications including trait detection during breeding, pest surveillance, and pathogen identification. Here, we use a modified version of the CRISPR-based nucleic acid detection platform SHERLOCK to quantify levels of a glyphosate resistance gene in a mixture of soybeans and to detect multiple plant genes in a single reaction. SHERLOCK is rapid (∼15 min), quantitative, and portable, and can process crude soybean extracts as input material for minimal nucleic acid sample preparation. This field-ready SHERLOCK platform with color-based lateral flow readout can be applied for detection and quantitation of genes in a range of agricultural applications. 2021-12-08T17:52:17Z 2021-12-08T17:52:17Z 2019 2021-12-08T17:49:10Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/138384 Abudayyeh, Omar O, Gootenberg, Jonathan S, Kellner, Max J and Zhang, Feng. 2019. "Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13." The CRISPR Journal, 2 (3). en 10.1089/CRISPR.2019.0011 The CRISPR Journal Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Mary Ann Liebert Inc Mary Ann Liebert |
| spellingShingle | Abudayyeh, Omar O Gootenberg, Jonathan S Kellner, Max J Zhang, Feng Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13 |
| title | Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13 |
| title_full | Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13 |
| title_fullStr | Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13 |
| title_full_unstemmed | Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13 |
| title_short | Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13 |
| title_sort | nucleic acid detection of plant genes using crispr cas13 |
| url | https://hdl.handle.net/1721.1/138384 |
| work_keys_str_mv | AT abudayyehomaro nucleicaciddetectionofplantgenesusingcrisprcas13 AT gootenbergjonathans nucleicaciddetectionofplantgenesusingcrisprcas13 AT kellnermaxj nucleicaciddetectionofplantgenesusingcrisprcas13 AT zhangfeng nucleicaciddetectionofplantgenesusingcrisprcas13 |