Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium Grasses
Dehydration proteins (dehydrins, DHNs) confer tolerance to water-stress deficit in plants. We performed a comparative genomics and evolutionary study of DHN genes in four model <i>Brachypodium</i> grass species. Due to limited knowledge on dehydrin expression under water deprivation stre...
| Main Authors: | , , , , , , , |
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| Format: | Article |
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Multidisciplinary Digital Publishing Institute
2021
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| Online Access: | https://hdl.handle.net/1721.1/138414 |
| _version_ | 1811088175719776256 |
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| author | Decena, Maria Angeles Gálvez-Rojas, Sergio Agostini, Federico Sancho, Ruben Contreras-Moreira, Bruno Des Marais, David L. Hernandez, Pilar Catalán, Pilar |
| author_facet | Decena, Maria Angeles Gálvez-Rojas, Sergio Agostini, Federico Sancho, Ruben Contreras-Moreira, Bruno Des Marais, David L. Hernandez, Pilar Catalán, Pilar |
| author_sort | Decena, Maria Angeles |
| collection | MIT |
| description | Dehydration proteins (dehydrins, DHNs) confer tolerance to water-stress deficit in plants. We performed a comparative genomics and evolutionary study of DHN genes in four model <i>Brachypodium</i> grass species. Due to limited knowledge on dehydrin expression under water deprivation stress in <i>Brachypodium,</i> we also performed a drought-induced gene expression analysis in 32 ecotypes of the genus’ flagship species <i>B. distachyon</i> showing different hydric requirements. Genomic sequence analysis detected 10 types of dehydrin genes (<i>Bdhn</i>) across the <i>Brachypodium</i> species. Domain and conserved motif contents of peptides encoded by <i>Bdhn</i> genes revealed eight protein architectures. <i>Bdhn</i> genes were spread across several chromosomes. Selection analysis indicated that all the <i>Bdhn</i> genes were constrained by purifying selection. Three upstream <i>cis</i>-regulatory motifs (BES1, MYB124, ZAT) were detected in several <i>Bdhn</i> genes. Gene expression analysis demonstrated that only four <i>Bdhn</i>1-<i>Bdhn</i>2, <i>Bdhn</i>3, and <i>Bdhn</i>7 genes, orthologs of wheat, barley, rice, sorghum, and maize genes, were expressed in mature leaves of <i>B. distachyon</i> and that all of them were more highly expressed in plants under drought conditions. <i>Brachypodium</i> dehydrin expression was significantly correlated with drought-response phenotypic traits (plant biomass, leaf carbon and proline contents and water use efficiency increases, and leaf water and nitrogen content decreases) being more pronounced in drought-tolerant ecotypes. Our results indicate that dehydrin type and regulation could be a key factor determining the acquisition of water-stress tolerance in grasses. |
| first_indexed | 2024-09-23T13:57:27Z |
| format | Article |
| id | mit-1721.1/138414 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T13:57:27Z |
| publishDate | 2021 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | mit-1721.1/1384142021-12-10T03:15:20Z Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium Grasses Decena, Maria Angeles Gálvez-Rojas, Sergio Agostini, Federico Sancho, Ruben Contreras-Moreira, Bruno Des Marais, David L. Hernandez, Pilar Catalán, Pilar Dehydration proteins (dehydrins, DHNs) confer tolerance to water-stress deficit in plants. We performed a comparative genomics and evolutionary study of DHN genes in four model <i>Brachypodium</i> grass species. Due to limited knowledge on dehydrin expression under water deprivation stress in <i>Brachypodium,</i> we also performed a drought-induced gene expression analysis in 32 ecotypes of the genus’ flagship species <i>B. distachyon</i> showing different hydric requirements. Genomic sequence analysis detected 10 types of dehydrin genes (<i>Bdhn</i>) across the <i>Brachypodium</i> species. Domain and conserved motif contents of peptides encoded by <i>Bdhn</i> genes revealed eight protein architectures. <i>Bdhn</i> genes were spread across several chromosomes. Selection analysis indicated that all the <i>Bdhn</i> genes were constrained by purifying selection. Three upstream <i>cis</i>-regulatory motifs (BES1, MYB124, ZAT) were detected in several <i>Bdhn</i> genes. Gene expression analysis demonstrated that only four <i>Bdhn</i>1-<i>Bdhn</i>2, <i>Bdhn</i>3, and <i>Bdhn</i>7 genes, orthologs of wheat, barley, rice, sorghum, and maize genes, were expressed in mature leaves of <i>B. distachyon</i> and that all of them were more highly expressed in plants under drought conditions. <i>Brachypodium</i> dehydrin expression was significantly correlated with drought-response phenotypic traits (plant biomass, leaf carbon and proline contents and water use efficiency increases, and leaf water and nitrogen content decreases) being more pronounced in drought-tolerant ecotypes. Our results indicate that dehydrin type and regulation could be a key factor determining the acquisition of water-stress tolerance in grasses. 2021-12-09T20:20:59Z 2021-12-09T20:20:59Z 2021-12-03 2021-12-09T14:32:22Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/138414 Plants 10 (12): 2664 (2021) PUBLISHER_CC http://dx.doi.org/10.3390/plants10122664 Creative Commons Attribution https://creativecommons.org/licenses/by/4.0/ application/pdf Multidisciplinary Digital Publishing Institute Multidisciplinary Digital Publishing Institute |
| spellingShingle | Decena, Maria Angeles Gálvez-Rojas, Sergio Agostini, Federico Sancho, Ruben Contreras-Moreira, Bruno Des Marais, David L. Hernandez, Pilar Catalán, Pilar Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium Grasses |
| title | Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium Grasses |
| title_full | Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium Grasses |
| title_fullStr | Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium Grasses |
| title_full_unstemmed | Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium Grasses |
| title_short | Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium Grasses |
| title_sort | comparative genomics evolution and drought induced expression of dehydrin genes in model brachypodium grasses |
| url | https://hdl.handle.net/1721.1/138414 |
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