High spatial and temporal resolution synthetic aperture phase microscopy
A new optical microscopy technique, termed high spatial and temporal resolution synthetic aperture phase microscopy (HISTR-SAPM), is proposed to improve the lateral resolution of wide-field coherent imaging. Under plane wave illumination, the resolution is increased by twofold to around 260 nm, whil...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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SPIE-Intl Soc Optical Eng
2022
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| Online Access: | https://hdl.handle.net/1721.1/139684 |
| _version_ | 1811072537705054208 |
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| author | Zheng, Cheng Jin, Di He, Yanping Lin, Hongtao Hu, Juejun Yaqoob, Zahid So, Peter TC Zhou, Renjie |
| author2 | Massachusetts Institute of Technology. Department of Mechanical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Mechanical Engineering Zheng, Cheng Jin, Di He, Yanping Lin, Hongtao Hu, Juejun Yaqoob, Zahid So, Peter TC Zhou, Renjie |
| author_sort | Zheng, Cheng |
| collection | MIT |
| description | A new optical microscopy technique, termed high spatial and temporal resolution synthetic aperture phase microscopy (HISTR-SAPM), is proposed to improve the lateral resolution of wide-field coherent imaging. Under plane wave illumination, the resolution is increased by twofold to around 260 nm, while achieving millisecond-level temporal resolution. In HISTR-SAPM, digital micromirror devices are used to actively change the sample illumination beam angle at high speed with high stability. An off-axis interferometer is used to measure the sample scattered complex fields, which are then processed to reconstruct high-resolution phase images. Using HISTR-SAPM, we are able to map the height profiles of subwavelength photonic structures and resolve the period structures that have 198 nm linewidth and 132 nm gap (i.e., a full pitch of 330 nm). As the reconstruction averages out laser speckle noise while maintaining high temporal resolution, HISTR-SAPM further enables imaging and quantification of nanoscale dynamics of live cells, such as red blood cell membrane fluctuations and subcellular structure dynamics within nucleated cells. We envision that HISTR-SAPM will broadly benefit research in material science and biology. |
| first_indexed | 2024-09-23T09:07:30Z |
| format | Article |
| id | mit-1721.1/139684 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T09:07:30Z |
| publishDate | 2022 |
| publisher | SPIE-Intl Soc Optical Eng |
| record_format | dspace |
| spelling | mit-1721.1/1396842023-02-09T20:11:55Z High spatial and temporal resolution synthetic aperture phase microscopy Zheng, Cheng Jin, Di He, Yanping Lin, Hongtao Hu, Juejun Yaqoob, Zahid So, Peter TC Zhou, Renjie Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Department of Materials Science and Engineering Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory Massachusetts Institute of Technology. Laser Biomedical Research Center Massachusetts Institute of Technology. Department of Biological Engineering A new optical microscopy technique, termed high spatial and temporal resolution synthetic aperture phase microscopy (HISTR-SAPM), is proposed to improve the lateral resolution of wide-field coherent imaging. Under plane wave illumination, the resolution is increased by twofold to around 260 nm, while achieving millisecond-level temporal resolution. In HISTR-SAPM, digital micromirror devices are used to actively change the sample illumination beam angle at high speed with high stability. An off-axis interferometer is used to measure the sample scattered complex fields, which are then processed to reconstruct high-resolution phase images. Using HISTR-SAPM, we are able to map the height profiles of subwavelength photonic structures and resolve the period structures that have 198 nm linewidth and 132 nm gap (i.e., a full pitch of 330 nm). As the reconstruction averages out laser speckle noise while maintaining high temporal resolution, HISTR-SAPM further enables imaging and quantification of nanoscale dynamics of live cells, such as red blood cell membrane fluctuations and subcellular structure dynamics within nucleated cells. We envision that HISTR-SAPM will broadly benefit research in material science and biology. 2022-01-25T13:44:39Z 2022-01-25T13:44:39Z 2020 2022-01-25T13:41:49Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/139684 Zheng, Cheng, Jin, Di, He, Yanping, Lin, Hongtao, Hu, Juejun et al. 2020. "High spatial and temporal resolution synthetic aperture phase microscopy." Advanced Photonics, 2 (06). en 10.1117/1.AP.2.6.065002 Advanced Photonics Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf SPIE-Intl Soc Optical Eng SPIE |
| spellingShingle | Zheng, Cheng Jin, Di He, Yanping Lin, Hongtao Hu, Juejun Yaqoob, Zahid So, Peter TC Zhou, Renjie High spatial and temporal resolution synthetic aperture phase microscopy |
| title | High spatial and temporal resolution synthetic aperture phase microscopy |
| title_full | High spatial and temporal resolution synthetic aperture phase microscopy |
| title_fullStr | High spatial and temporal resolution synthetic aperture phase microscopy |
| title_full_unstemmed | High spatial and temporal resolution synthetic aperture phase microscopy |
| title_short | High spatial and temporal resolution synthetic aperture phase microscopy |
| title_sort | high spatial and temporal resolution synthetic aperture phase microscopy |
| url | https://hdl.handle.net/1721.1/139684 |
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