Hydrogel-based biocontainment of bacteria for continuous sensing and computation
Genetically modified microorganisms (GMMs) can enable a wide range of important applications including environmental sensing and responsive engineered living materials. However, containment of GMMs to prevent environmental escape and satisfy regulatory requirements is a bottleneck for real-world use...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer Science and Business Media LLC
2022
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| Online Access: | https://hdl.handle.net/1721.1/139764 |
| _version_ | 1826198030600110080 |
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| author | Tang, Tzu-Chieh Tham, Eléonore Liu, Xinyue Yehl, Kevin Rovner, Alexis J Yuk, Hyunwoo de la Fuente-Nunez, Cesar Isaacs, Farren J Zhao, Xuanhe Lu, Timothy K |
| author_facet | Tang, Tzu-Chieh Tham, Eléonore Liu, Xinyue Yehl, Kevin Rovner, Alexis J Yuk, Hyunwoo de la Fuente-Nunez, Cesar Isaacs, Farren J Zhao, Xuanhe Lu, Timothy K |
| author_sort | Tang, Tzu-Chieh |
| collection | MIT |
| description | Genetically modified microorganisms (GMMs) can enable a wide range of important applications including environmental sensing and responsive engineered living materials. However, containment of GMMs to prevent environmental escape and satisfy regulatory requirements is a bottleneck for real-world use. While current biochemical strategies restrict unwanted growth of GMMs in the environment, there is a need for deployable physical containment technologies to achieve redundant, multi-layered and robust containment. We developed a hydrogel-based encapsulation system that incorporates a biocompatible multilayer tough shell and an alginate-based core. This deployable physical containment strategy (DEPCOS) allows no detectable GMM escape, bacteria to be protected against environmental insults including antibiotics and low pH, controllable lifespan and easy retrieval of genomically recoded bacteria. To highlight the versatility of DEPCOS, we demonstrated that robustly encapsulated cells can execute useful functions, including performing cell-cell communication with other encapsulated bacteria and sensing heavy metals in water samples from the Charles River. |
| first_indexed | 2024-09-23T10:57:46Z |
| format | Article |
| id | mit-1721.1/139764 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T10:57:46Z |
| publishDate | 2022 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1397642022-01-28T03:27:18Z Hydrogel-based biocontainment of bacteria for continuous sensing and computation Tang, Tzu-Chieh Tham, Eléonore Liu, Xinyue Yehl, Kevin Rovner, Alexis J Yuk, Hyunwoo de la Fuente-Nunez, Cesar Isaacs, Farren J Zhao, Xuanhe Lu, Timothy K Genetically modified microorganisms (GMMs) can enable a wide range of important applications including environmental sensing and responsive engineered living materials. However, containment of GMMs to prevent environmental escape and satisfy regulatory requirements is a bottleneck for real-world use. While current biochemical strategies restrict unwanted growth of GMMs in the environment, there is a need for deployable physical containment technologies to achieve redundant, multi-layered and robust containment. We developed a hydrogel-based encapsulation system that incorporates a biocompatible multilayer tough shell and an alginate-based core. This deployable physical containment strategy (DEPCOS) allows no detectable GMM escape, bacteria to be protected against environmental insults including antibiotics and low pH, controllable lifespan and easy retrieval of genomically recoded bacteria. To highlight the versatility of DEPCOS, we demonstrated that robustly encapsulated cells can execute useful functions, including performing cell-cell communication with other encapsulated bacteria and sensing heavy metals in water samples from the Charles River. 2022-01-27T14:15:15Z 2022-01-27T14:15:15Z 2021 2022-01-27T14:11:31Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/139764 Tang, Tzu-Chieh, Tham, Eléonore, Liu, Xinyue, Yehl, Kevin, Rovner, Alexis J et al. 2021. "Hydrogel-based biocontainment of bacteria for continuous sensing and computation." Nature Chemical Biology, 17 (6). en 10.1038/S41589-021-00779-6 Nature Chemical Biology Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Springer Science and Business Media LLC Other repository |
| spellingShingle | Tang, Tzu-Chieh Tham, Eléonore Liu, Xinyue Yehl, Kevin Rovner, Alexis J Yuk, Hyunwoo de la Fuente-Nunez, Cesar Isaacs, Farren J Zhao, Xuanhe Lu, Timothy K Hydrogel-based biocontainment of bacteria for continuous sensing and computation |
| title | Hydrogel-based biocontainment of bacteria for continuous sensing and computation |
| title_full | Hydrogel-based biocontainment of bacteria for continuous sensing and computation |
| title_fullStr | Hydrogel-based biocontainment of bacteria for continuous sensing and computation |
| title_full_unstemmed | Hydrogel-based biocontainment of bacteria for continuous sensing and computation |
| title_short | Hydrogel-based biocontainment of bacteria for continuous sensing and computation |
| title_sort | hydrogel based biocontainment of bacteria for continuous sensing and computation |
| url | https://hdl.handle.net/1721.1/139764 |
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