Modular Integration of Hydrogel Neural Interfaces
Thermal drawing has been recently leveraged to yield multifunctional, fiber-based neural probes at near kilometer length scales. Despite its promise, the widespread adoption of this approach has been impeded by (1) material compatibility requirements and (2) labor-intensive interfacing of functional...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Chemical Society (ACS)
2022
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| Online Access: | https://hdl.handle.net/1721.1/139769 |
| _version_ | 1826216104204173312 |
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| author | Tabet, Anthony Antonini, Marc-Joseph Sahasrabudhe, Atharva Park, Jimin Rosenfeld, Dekel Koehler, Florian Yuk, Hyunwoo Hanson, Samuel Stinson, Jordan Stok, Melissa Zhao, Xuanhe Wang, Chun Anikeeva, Polina |
| author2 | Massachusetts Institute of Technology. Department of Materials Science and Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Materials Science and Engineering Tabet, Anthony Antonini, Marc-Joseph Sahasrabudhe, Atharva Park, Jimin Rosenfeld, Dekel Koehler, Florian Yuk, Hyunwoo Hanson, Samuel Stinson, Jordan Stok, Melissa Zhao, Xuanhe Wang, Chun Anikeeva, Polina |
| author_sort | Tabet, Anthony |
| collection | MIT |
| description | Thermal drawing has been recently leveraged to yield multifunctional, fiber-based neural probes at near kilometer length scales. Despite its promise, the widespread adoption of this approach has been impeded by (1) material compatibility requirements and (2) labor-intensive interfacing of functional features to external hardware. Furthermore, in multifunctional fibers, significant volume is occupied by passive polymer cladding that so far has only served structural or electrical insulation purposes. In this article, we report a rapid, robust, and modular approach to creating multifunctional fiber-based neural interfaces using a solvent evaporation or entrapment-driven (SEED) integration process. This process brings together electrical, optical, and microfluidic modalities all encased within a copolymer comprised of water-soluble poly(ethylene glycol) tethered to water-insoluble poly(urethane) (PU-PEG). We employ these devices for simultaneous optogenetics and electrophysiology and demonstrate that multifunctional neural probes can be used to deliver cellular cargo with high viability. Upon exposure to water, PU-PEG cladding spontaneously forms a hydrogel, which in addition to enabling integration of modalities, can harbor small molecules and nanomaterials that can be released into local tissue following implantation. We also synthesized a custom nanodroplet forming block polymer and demonstrated that embedding such materials within the hydrogel cladding of our probes enables delivery of hydrophobic small molecules in vitro and in vivo. Our approach widens the chemical toolbox and expands the capabilities of multifunctional neural interfaces. |
| first_indexed | 2024-09-23T16:42:05Z |
| format | Article |
| id | mit-1721.1/139769 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T16:42:05Z |
| publishDate | 2022 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/1397692023-01-20T16:34:38Z Modular Integration of Hydrogel Neural Interfaces Tabet, Anthony Antonini, Marc-Joseph Sahasrabudhe, Atharva Park, Jimin Rosenfeld, Dekel Koehler, Florian Yuk, Hyunwoo Hanson, Samuel Stinson, Jordan Stok, Melissa Zhao, Xuanhe Wang, Chun Anikeeva, Polina Massachusetts Institute of Technology. Department of Materials Science and Engineering McGovern Institute for Brain Research at MIT Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Massachusetts Institute of Technology. Research Laboratory of Electronics Massachusetts Institute of Technology. Department of Chemical Engineering Koch Institute for Integrative Cancer Research at MIT Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Chemistry Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Department of Biological Engineering Thermal drawing has been recently leveraged to yield multifunctional, fiber-based neural probes at near kilometer length scales. Despite its promise, the widespread adoption of this approach has been impeded by (1) material compatibility requirements and (2) labor-intensive interfacing of functional features to external hardware. Furthermore, in multifunctional fibers, significant volume is occupied by passive polymer cladding that so far has only served structural or electrical insulation purposes. In this article, we report a rapid, robust, and modular approach to creating multifunctional fiber-based neural interfaces using a solvent evaporation or entrapment-driven (SEED) integration process. This process brings together electrical, optical, and microfluidic modalities all encased within a copolymer comprised of water-soluble poly(ethylene glycol) tethered to water-insoluble poly(urethane) (PU-PEG). We employ these devices for simultaneous optogenetics and electrophysiology and demonstrate that multifunctional neural probes can be used to deliver cellular cargo with high viability. Upon exposure to water, PU-PEG cladding spontaneously forms a hydrogel, which in addition to enabling integration of modalities, can harbor small molecules and nanomaterials that can be released into local tissue following implantation. We also synthesized a custom nanodroplet forming block polymer and demonstrated that embedding such materials within the hydrogel cladding of our probes enables delivery of hydrophobic small molecules in vitro and in vivo. Our approach widens the chemical toolbox and expands the capabilities of multifunctional neural interfaces. 2022-01-27T14:58:57Z 2022-01-27T14:58:57Z 2021 2022-01-27T14:53:14Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/139769 Tabet, Anthony, Antonini, Marc-Joseph, Sahasrabudhe, Atharva, Park, Jimin, Rosenfeld, Dekel et al. 2021. "Modular Integration of Hydrogel Neural Interfaces." ACS Central Science, 7 (9). en 10.1021/ACSCENTSCI.1C00592 ACS Central Science Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf American Chemical Society (ACS) ACS |
| spellingShingle | Tabet, Anthony Antonini, Marc-Joseph Sahasrabudhe, Atharva Park, Jimin Rosenfeld, Dekel Koehler, Florian Yuk, Hyunwoo Hanson, Samuel Stinson, Jordan Stok, Melissa Zhao, Xuanhe Wang, Chun Anikeeva, Polina Modular Integration of Hydrogel Neural Interfaces |
| title | Modular Integration of Hydrogel Neural Interfaces |
| title_full | Modular Integration of Hydrogel Neural Interfaces |
| title_fullStr | Modular Integration of Hydrogel Neural Interfaces |
| title_full_unstemmed | Modular Integration of Hydrogel Neural Interfaces |
| title_short | Modular Integration of Hydrogel Neural Interfaces |
| title_sort | modular integration of hydrogel neural interfaces |
| url | https://hdl.handle.net/1721.1/139769 |
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