Versatile Biological Sample Preparation Platform using Microfluidic Cell Sorting Device
Biological assays for various biological samples are often limited by the low purity of target cells/particles due to the presence of significant host background. A spiral inertial microfluidic cell sorting device can be used to separate cells/particles based on their sizes without any specific labe...
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| Format: | Thesis |
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Massachusetts Institute of Technology
2022
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| Online Access: | https://hdl.handle.net/1721.1/140073 https://orcid.org/0000-0003-0935-4158 |
| Summary: | Biological assays for various biological samples are often limited by the low purity of target cells/particles due to the presence of significant host background. A spiral inertial microfluidic cell sorting device can be used to separate cells/particles based on their sizes without any specific labeling and result in a purified sample with a higher purity of target particles, enabling a higher chance of detecting/analyzing those particles of interest for biological assays. Moreover, the cell-sorting ability of spiral devices can be applied as a cell-washing technology to isolate target cells while removing unwanted particles such as adventitious agents for a better quality of cellular products or manufacturing cells in biomanufacturing. This work provides numerous applications of spiral cell sorter-based microfluidic sample preparation on various biological samples. We propose to apply spiral microfluidic sorter for various biological samples to acquire enhanced readouts for targeted downstream assays such as Next-generation sequencing (NGS) or improved quality of cells for biomanufacturing. |
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