Versatile Biological Sample Preparation Platform using Microfluidic Cell Sorting Device
Biological assays for various biological samples are often limited by the low purity of target cells/particles due to the presence of significant host background. A spiral inertial microfluidic cell sorting device can be used to separate cells/particles based on their sizes without any specific labe...
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| Format: | Thesis |
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Massachusetts Institute of Technology
2022
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| Online Access: | https://hdl.handle.net/1721.1/140073 https://orcid.org/0000-0003-0935-4158 |
| _version_ | 1826206648442552320 |
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| author | Choi, Kyungyong |
| author2 | Han, Jongyoon |
| author_facet | Han, Jongyoon Choi, Kyungyong |
| author_sort | Choi, Kyungyong |
| collection | MIT |
| description | Biological assays for various biological samples are often limited by the low purity of target cells/particles due to the presence of significant host background. A spiral inertial microfluidic cell sorting device can be used to separate cells/particles based on their sizes without any specific labeling and result in a purified sample with a higher purity of target particles, enabling a higher chance of detecting/analyzing those particles of interest for biological assays. Moreover, the cell-sorting ability of spiral devices can be applied as a cell-washing technology to isolate target cells while removing unwanted particles such as adventitious agents for a better quality of cellular products or manufacturing cells in biomanufacturing. This work provides numerous applications of spiral cell sorter-based microfluidic sample preparation on various biological samples. We propose to apply spiral microfluidic sorter for various biological samples to acquire enhanced readouts for targeted downstream assays such as Next-generation sequencing (NGS) or improved quality of cells for biomanufacturing. |
| first_indexed | 2024-09-23T13:36:06Z |
| format | Thesis |
| id | mit-1721.1/140073 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T13:36:06Z |
| publishDate | 2022 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/1400732022-02-08T03:30:52Z Versatile Biological Sample Preparation Platform using Microfluidic Cell Sorting Device Choi, Kyungyong Han, Jongyoon Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Biological assays for various biological samples are often limited by the low purity of target cells/particles due to the presence of significant host background. A spiral inertial microfluidic cell sorting device can be used to separate cells/particles based on their sizes without any specific labeling and result in a purified sample with a higher purity of target particles, enabling a higher chance of detecting/analyzing those particles of interest for biological assays. Moreover, the cell-sorting ability of spiral devices can be applied as a cell-washing technology to isolate target cells while removing unwanted particles such as adventitious agents for a better quality of cellular products or manufacturing cells in biomanufacturing. This work provides numerous applications of spiral cell sorter-based microfluidic sample preparation on various biological samples. We propose to apply spiral microfluidic sorter for various biological samples to acquire enhanced readouts for targeted downstream assays such as Next-generation sequencing (NGS) or improved quality of cells for biomanufacturing. Ph.D. 2022-02-07T15:22:29Z 2022-02-07T15:22:29Z 2021-09 2021-09-21T19:30:43.295Z Thesis https://hdl.handle.net/1721.1/140073 https://orcid.org/0000-0003-0935-4158 In Copyright - Educational Use Permitted Copyright MIT http://rightsstatements.org/page/InC-EDU/1.0/ application/pdf Massachusetts Institute of Technology |
| spellingShingle | Choi, Kyungyong Versatile Biological Sample Preparation Platform using Microfluidic Cell Sorting Device |
| title | Versatile Biological Sample Preparation Platform using Microfluidic Cell Sorting Device |
| title_full | Versatile Biological Sample Preparation Platform using Microfluidic Cell Sorting Device |
| title_fullStr | Versatile Biological Sample Preparation Platform using Microfluidic Cell Sorting Device |
| title_full_unstemmed | Versatile Biological Sample Preparation Platform using Microfluidic Cell Sorting Device |
| title_short | Versatile Biological Sample Preparation Platform using Microfluidic Cell Sorting Device |
| title_sort | versatile biological sample preparation platform using microfluidic cell sorting device |
| url | https://hdl.handle.net/1721.1/140073 https://orcid.org/0000-0003-0935-4158 |
| work_keys_str_mv | AT choikyungyong versatilebiologicalsamplepreparationplatformusingmicrofluidiccellsortingdevice |