Microphysiological Neurovascular Barriers to Model the Inner Retinal Microvasculature
Blood-neural barriers regulate nutrient supply to neuronal tissues and prevent neurotoxicity. In particular, the inner blood-retinal barrier (iBRB) and blood–brain barrier (BBB) share common origins in development, and similar morphology and function in adult tissue, while barrier breakdow...
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| Format: | Article |
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MDPI AG
2022
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| Online Access: | https://hdl.handle.net/1721.1/140289 |
| _version_ | 1826199590599131136 |
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| author | Maurissen, Thomas L. Pavlou, Georgios Bichsel, Colette Villaseñor, Roberto Kamm, Roger D. Ragelle, Héloïse |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Maurissen, Thomas L. Pavlou, Georgios Bichsel, Colette Villaseñor, Roberto Kamm, Roger D. Ragelle, Héloïse |
| author_sort | Maurissen, Thomas L. |
| collection | MIT |
| description | Blood-neural barriers regulate nutrient supply to neuronal tissues and prevent neurotoxicity. In particular, the inner blood-retinal barrier (iBRB) and blood–brain barrier (BBB) share common origins in development, and similar morphology and function in adult tissue, while barrier breakdown and leakage of neurotoxic molecules can be accompanied by neurodegeneration. Therefore, pre-clinical research requires human in vitro models that elucidate pathophysiological mechanisms and support drug discovery, to add to animal in vivo modeling that poorly predict patient responses. Advanced cellular models such as microphysiological systems (MPS) recapitulate tissue organization and function in many organ-specific contexts, providing physiological relevance, potential for customization to different population groups, and scalability for drug screening purposes. While human-based MPS have been developed for tissues such as lung, gut, brain and tumors, few comprehensive models exist for ocular tissues and iBRB modeling. Recent BBB in vitro models using human cells of the neurovascular unit (NVU) showed physiological morphology and permeability values, and reproduced brain neurological disorder phenotypes that could be applicable to modeling the iBRB. Here, we describe similarities between iBRB and BBB properties, compare existing neurovascular barrier models, propose leverage of MPS-based strategies to develop new iBRB models, and explore potentials to personalize cellular inputs and improve pre-clinical testing. |
| first_indexed | 2024-09-23T11:22:21Z |
| format | Article |
| id | mit-1721.1/140289 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T11:22:21Z |
| publishDate | 2022 |
| publisher | MDPI AG |
| record_format | dspace |
| spelling | mit-1721.1/1402892024-06-07T17:37:35Z Microphysiological Neurovascular Barriers to Model the Inner Retinal Microvasculature Maurissen, Thomas L. Pavlou, Georgios Bichsel, Colette Villaseñor, Roberto Kamm, Roger D. Ragelle, Héloïse Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Blood-neural barriers regulate nutrient supply to neuronal tissues and prevent neurotoxicity. In particular, the inner blood-retinal barrier (iBRB) and blood–brain barrier (BBB) share common origins in development, and similar morphology and function in adult tissue, while barrier breakdown and leakage of neurotoxic molecules can be accompanied by neurodegeneration. Therefore, pre-clinical research requires human in vitro models that elucidate pathophysiological mechanisms and support drug discovery, to add to animal in vivo modeling that poorly predict patient responses. Advanced cellular models such as microphysiological systems (MPS) recapitulate tissue organization and function in many organ-specific contexts, providing physiological relevance, potential for customization to different population groups, and scalability for drug screening purposes. While human-based MPS have been developed for tissues such as lung, gut, brain and tumors, few comprehensive models exist for ocular tissues and iBRB modeling. Recent BBB in vitro models using human cells of the neurovascular unit (NVU) showed physiological morphology and permeability values, and reproduced brain neurological disorder phenotypes that could be applicable to modeling the iBRB. Here, we describe similarities between iBRB and BBB properties, compare existing neurovascular barrier models, propose leverage of MPS-based strategies to develop new iBRB models, and explore potentials to personalize cellular inputs and improve pre-clinical testing. 2022-02-11T17:22:00Z 2022-02-11T17:22:00Z 2022-01-24 2022-02-11T14:46:24Z Article http://purl.org/eprint/type/JournalArticle 2075-4426 https://hdl.handle.net/1721.1/140289 Maurissen TL, Pavlou G, Bichsel C, Villaseñor R, Kamm RD, Ragelle H. Microphysiological Neurovascular Barriers to Model the Inner Retinal Microvasculature. Journal of Personalized Medicine. 2022; 12(2):148 10.3390/jpm12020148 Journal of Personalized Medicine Creative Commons Attribution https://creativecommons.org/licenses/by/4.0/ application/pdf MDPI AG Multidisciplinary Digital Publishing Institute |
| spellingShingle | Maurissen, Thomas L. Pavlou, Georgios Bichsel, Colette Villaseñor, Roberto Kamm, Roger D. Ragelle, Héloïse Microphysiological Neurovascular Barriers to Model the Inner Retinal Microvasculature |
| title | Microphysiological Neurovascular Barriers to Model the Inner Retinal Microvasculature |
| title_full | Microphysiological Neurovascular Barriers to Model the Inner Retinal Microvasculature |
| title_fullStr | Microphysiological Neurovascular Barriers to Model the Inner Retinal Microvasculature |
| title_full_unstemmed | Microphysiological Neurovascular Barriers to Model the Inner Retinal Microvasculature |
| title_short | Microphysiological Neurovascular Barriers to Model the Inner Retinal Microvasculature |
| title_sort | microphysiological neurovascular barriers to model the inner retinal microvasculature |
| url | https://hdl.handle.net/1721.1/140289 |
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