Implantable Nanosensors for Human Steroid Hormone Sensing In Vivo Using a Self‐Templating Corona Phase Molecular Recognition
Dynamic measurements of steroid hormones in vivo are critical, but steroid sensing is currently limited by the availability of specific molecular recognition elements due to the chemical similarity of these hormones. In this work, a new, self-templating synthetic approach is applied using corona pha...
| Main Authors: | , , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
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Wiley
2022
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| Online Access: | https://hdl.handle.net/1721.1/140428 |
| _version_ | 1826193000085061632 |
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| author | Lee, Michael A. Wang, Song Jin, Xiaojia Bakh, Naveed Ali Nguyen, Freddy T. Dong, Juyao Silmore, Kevin S. Gong, Xun Pham, Crystal Jones, Kelvin K. Muthupalani, Sureshkumar Bisker, Gili Son, Manki Strano, Michael S. |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Lee, Michael A. Wang, Song Jin, Xiaojia Bakh, Naveed Ali Nguyen, Freddy T. Dong, Juyao Silmore, Kevin S. Gong, Xun Pham, Crystal Jones, Kelvin K. Muthupalani, Sureshkumar Bisker, Gili Son, Manki Strano, Michael S. |
| author_sort | Lee, Michael A. |
| collection | MIT |
| description | Dynamic measurements of steroid hormones in vivo are critical, but steroid sensing is currently limited by the availability of specific molecular recognition elements due to the chemical similarity of these hormones. In this work, a new, self-templating synthetic approach is applied using corona phase molecular recognition (CoPhMoRe) targeting the steroid family of molecules to produce near infrared fluorescent, implantable sensors. A key limitation of CoPhMoRe has been its reliance on library generation for sensor screening. This problem is addressed with a self-templating strategy of polymer design, using the examples of progesterone and cortisol sensing based on a styrene and acrylic acid copolymer library augmented with an acrylated steroid. The pendant steroid attached to the corona backbone is shown to self-template the phase, providing a unique CoPhMoRE design strategy with high efficacy. The resulting sensors exhibit excellent stability and reversibility upon repeated analyte cycling. It is shown that molecular recognition using such constructs is viable even in vivo after sensor implantation into a murine model by employing a poly (ethylene glycol) diacrylate (PEGDA) hydrogel and porous cellulose interface to limit nonspecific absorption. The results demonstrate that CoPhMoRe templating is sufficiently robust to enable a new class of continuous, in vivo biosensors. |
| first_indexed | 2024-09-23T09:32:08Z |
| format | Article |
| id | mit-1721.1/140428 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T09:32:08Z |
| publishDate | 2022 |
| publisher | Wiley |
| record_format | dspace |
| spelling | mit-1721.1/1404282024-06-06T20:09:56Z Implantable Nanosensors for Human Steroid Hormone Sensing In Vivo Using a Self‐Templating Corona Phase Molecular Recognition Lee, Michael A. Wang, Song Jin, Xiaojia Bakh, Naveed Ali Nguyen, Freddy T. Dong, Juyao Silmore, Kevin S. Gong, Xun Pham, Crystal Jones, Kelvin K. Muthupalani, Sureshkumar Bisker, Gili Son, Manki Strano, Michael S. Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Division of Comparative Medicine Dynamic measurements of steroid hormones in vivo are critical, but steroid sensing is currently limited by the availability of specific molecular recognition elements due to the chemical similarity of these hormones. In this work, a new, self-templating synthetic approach is applied using corona phase molecular recognition (CoPhMoRe) targeting the steroid family of molecules to produce near infrared fluorescent, implantable sensors. A key limitation of CoPhMoRe has been its reliance on library generation for sensor screening. This problem is addressed with a self-templating strategy of polymer design, using the examples of progesterone and cortisol sensing based on a styrene and acrylic acid copolymer library augmented with an acrylated steroid. The pendant steroid attached to the corona backbone is shown to self-template the phase, providing a unique CoPhMoRE design strategy with high efficacy. The resulting sensors exhibit excellent stability and reversibility upon repeated analyte cycling. It is shown that molecular recognition using such constructs is viable even in vivo after sensor implantation into a murine model by employing a poly (ethylene glycol) diacrylate (PEGDA) hydrogel and porous cellulose interface to limit nonspecific absorption. The results demonstrate that CoPhMoRe templating is sufficiently robust to enable a new class of continuous, in vivo biosensors. 2022-02-16T18:21:40Z 2022-02-16T18:21:40Z 2020-09-16 Article http://purl.org/eprint/type/JournalArticle 2192-2640 2192-2659 https://hdl.handle.net/1721.1/140428 Lee, M. A., Wang, S., Jin, X., Bakh, N. A., Nguyen, F. T., Dong, J., Silmore, K. S., Gong, X., Pham, C., Jones, K. K., Muthupalani, S., Bisker, G., Son, M., Strano, M. S., Implantable Nanosensors for Human Steroid Hormone Sensing In Vivo Using a Self-Templating Corona Phase Molecular Recognition. Adv. Healthcare Mater. 2020, 9, 2000429. en http://dx.doi.org/10.1002/adhm.202000429 Advanced Healthcare Materials Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Wiley Wiley |
| spellingShingle | Lee, Michael A. Wang, Song Jin, Xiaojia Bakh, Naveed Ali Nguyen, Freddy T. Dong, Juyao Silmore, Kevin S. Gong, Xun Pham, Crystal Jones, Kelvin K. Muthupalani, Sureshkumar Bisker, Gili Son, Manki Strano, Michael S. Implantable Nanosensors for Human Steroid Hormone Sensing In Vivo Using a Self‐Templating Corona Phase Molecular Recognition |
| title | Implantable Nanosensors for Human Steroid Hormone Sensing In Vivo Using a Self‐Templating Corona Phase Molecular Recognition |
| title_full | Implantable Nanosensors for Human Steroid Hormone Sensing In Vivo Using a Self‐Templating Corona Phase Molecular Recognition |
| title_fullStr | Implantable Nanosensors for Human Steroid Hormone Sensing In Vivo Using a Self‐Templating Corona Phase Molecular Recognition |
| title_full_unstemmed | Implantable Nanosensors for Human Steroid Hormone Sensing In Vivo Using a Self‐Templating Corona Phase Molecular Recognition |
| title_short | Implantable Nanosensors for Human Steroid Hormone Sensing In Vivo Using a Self‐Templating Corona Phase Molecular Recognition |
| title_sort | implantable nanosensors for human steroid hormone sensing in vivo using a self templating corona phase molecular recognition |
| url | https://hdl.handle.net/1721.1/140428 |
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