Polysaccharide length affects mycobacterial cell shape and antibiotic susceptibility
Copyright © 2020 The Authors, some rights reserved. Bacteria control the length of their polysaccharides, which can control cell viability, physiology, virulence, and immune evasion. Polysaccharide chain length affects immunomodulation, but its impact on bacterial physiology and antibiotic susceptib...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Association for the Advancement of Science (AAAS)
2022
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| Online Access: | https://hdl.handle.net/1721.1/141095 |
| _version_ | 1826208228650778624 |
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| author | Justen, Alexander M Hodges, Heather L Kim, Lili M Sadecki, Patric W Porfirio, Sara Ultee, Eveline Black, Ian Chung, Grace S Briegel, Ariane Azadi, Parastoo Kiessling, Laura L |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Justen, Alexander M Hodges, Heather L Kim, Lili M Sadecki, Patric W Porfirio, Sara Ultee, Eveline Black, Ian Chung, Grace S Briegel, Ariane Azadi, Parastoo Kiessling, Laura L |
| author_sort | Justen, Alexander M |
| collection | MIT |
| description | Copyright © 2020 The Authors, some rights reserved. Bacteria control the length of their polysaccharides, which can control cell viability, physiology, virulence, and immune evasion. Polysaccharide chain length affects immunomodulation, but its impact on bacterial physiology and antibiotic susceptibility was unclear. We probed the consequences of truncating the mycobacterial galactan, an essential linear polysaccharide of about 30 residues. Galactan covalently bridges cell envelope layers, with the outermost cell wall linkage point occurring at residue 12. Reducing galactan chain length by approximately half compromises fitness, alters cell morphology, and increases the potency of hydrophobic antibiotics. Systematic variation of the galactan chain length revealed that it determines periplasm size. Thus, glycan chain length can directly affect cellular physiology and antibiotic activity, and mycobacterial glycans, not proteins, regulate periplasm size. |
| first_indexed | 2024-09-23T14:02:32Z |
| format | Article |
| id | mit-1721.1/141095 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T14:02:32Z |
| publishDate | 2022 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | dspace |
| spelling | mit-1721.1/1410952023-06-20T17:30:47Z Polysaccharide length affects mycobacterial cell shape and antibiotic susceptibility Justen, Alexander M Hodges, Heather L Kim, Lili M Sadecki, Patric W Porfirio, Sara Ultee, Eveline Black, Ian Chung, Grace S Briegel, Ariane Azadi, Parastoo Kiessling, Laura L Massachusetts Institute of Technology. Department of Chemistry Copyright © 2020 The Authors, some rights reserved. Bacteria control the length of their polysaccharides, which can control cell viability, physiology, virulence, and immune evasion. Polysaccharide chain length affects immunomodulation, but its impact on bacterial physiology and antibiotic susceptibility was unclear. We probed the consequences of truncating the mycobacterial galactan, an essential linear polysaccharide of about 30 residues. Galactan covalently bridges cell envelope layers, with the outermost cell wall linkage point occurring at residue 12. Reducing galactan chain length by approximately half compromises fitness, alters cell morphology, and increases the potency of hydrophobic antibiotics. Systematic variation of the galactan chain length revealed that it determines periplasm size. Thus, glycan chain length can directly affect cellular physiology and antibiotic activity, and mycobacterial glycans, not proteins, regulate periplasm size. 2022-03-09T17:46:32Z 2022-03-09T17:46:32Z 2020 2022-03-09T17:34:33Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/141095 Justen, Alexander M, Hodges, Heather L, Kim, Lili M, Sadecki, Patric W, Porfirio, Sara et al. 2020. "Polysaccharide length affects mycobacterial cell shape and antibiotic susceptibility." Science Advances, 6 (38). en 10.1126/SCIADV.ABA4015 Science Advances Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf American Association for the Advancement of Science (AAAS) Science Advances |
| spellingShingle | Justen, Alexander M Hodges, Heather L Kim, Lili M Sadecki, Patric W Porfirio, Sara Ultee, Eveline Black, Ian Chung, Grace S Briegel, Ariane Azadi, Parastoo Kiessling, Laura L Polysaccharide length affects mycobacterial cell shape and antibiotic susceptibility |
| title | Polysaccharide length affects mycobacterial cell shape and antibiotic susceptibility |
| title_full | Polysaccharide length affects mycobacterial cell shape and antibiotic susceptibility |
| title_fullStr | Polysaccharide length affects mycobacterial cell shape and antibiotic susceptibility |
| title_full_unstemmed | Polysaccharide length affects mycobacterial cell shape and antibiotic susceptibility |
| title_short | Polysaccharide length affects mycobacterial cell shape and antibiotic susceptibility |
| title_sort | polysaccharide length affects mycobacterial cell shape and antibiotic susceptibility |
| url | https://hdl.handle.net/1721.1/141095 |
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