Identification of N-Terminally Diversified GLP-1R Agonists Using Saturation Mutagenesis and Chemical Design
© The glucagon-like peptide 1 receptor (GLP-1R) is a class B G-protein coupled receptor (GPCR) and diabetes drug target expressed mainly in pancreatic β-cells that, when activated by its agonist glucagon-like peptide 1 (GLP-1) after a meal, stimulates insulin secretion and β-cell survival and proli...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
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American Chemical Society (ACS)
2022
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| Online Access: | https://hdl.handle.net/1721.1/141201.2 |
| _version_ | 1811091354427588608 |
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| author | Longwell, Chelsea K. Hanna, Stephanie Hartrampf, Nina Sperberg, R. Andres Parra Huang, Po-Ssu Pentelute, Bradley L. Cochran, Jennifer R. |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Longwell, Chelsea K. Hanna, Stephanie Hartrampf, Nina Sperberg, R. Andres Parra Huang, Po-Ssu Pentelute, Bradley L. Cochran, Jennifer R. |
| author_sort | Longwell, Chelsea K. |
| collection | MIT |
| description | © The glucagon-like peptide 1 receptor (GLP-1R) is a class B G-protein coupled receptor (GPCR) and diabetes drug target expressed mainly in pancreatic β-cells that, when activated by its agonist glucagon-like peptide 1 (GLP-1) after a meal, stimulates insulin secretion and β-cell survival and proliferation. The N-terminal region of GLP-1 interacts with membrane-proximal residues of GLP-1R, stabilizing its active conformation to trigger intracellular signaling. The best-studied agonist peptides, GLP-1 and exendin-4, share sequence homology at their N-terminal region; however, modifications that can be tolerated here are not fully understood. In this work, a functional screen of GLP-1 variants with randomized N-terminal domains reveals new GLP-1R agonists and uncovers a pattern whereby a negative charge is preferred at the third position in various sequence contexts. We further tested this sequence-structure-activity principle by synthesizing peptide analogues where this position was mutated to both canonical and noncanonical amino acids. We discovered a highly active GLP-1 analogue in which the native glutamate residue three positions from the N-terminus was replaced with the sulfo-containing amino acid cysteic acid (GLP-1-CYA). The receptor binding and downstream signaling properties elicited by GLP-1-CYA were similar to the wild type GLP-1 peptide. Computational modeling identified a likely mode of interaction of the negatively charged side chain in GLP-1-CYA with an arginine on GLP-1R. This work highlights a strategy of combinatorial peptide screening coupled with chemical exploration that could be used to generate novel agonists for other receptors with peptide ligands. |
| first_indexed | 2024-09-23T15:01:09Z |
| format | Article |
| id | mit-1721.1/141201.2 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T15:01:09Z |
| publishDate | 2022 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/141201.22024-06-14T16:26:35Z Identification of N-Terminally Diversified GLP-1R Agonists Using Saturation Mutagenesis and Chemical Design Longwell, Chelsea K. Hanna, Stephanie Hartrampf, Nina Sperberg, R. Andres Parra Huang, Po-Ssu Pentelute, Bradley L. Cochran, Jennifer R. Massachusetts Institute of Technology. Department of Chemistry Massachusetts Institute of Technology. Center for Environmental Health Sciences © The glucagon-like peptide 1 receptor (GLP-1R) is a class B G-protein coupled receptor (GPCR) and diabetes drug target expressed mainly in pancreatic β-cells that, when activated by its agonist glucagon-like peptide 1 (GLP-1) after a meal, stimulates insulin secretion and β-cell survival and proliferation. The N-terminal region of GLP-1 interacts with membrane-proximal residues of GLP-1R, stabilizing its active conformation to trigger intracellular signaling. The best-studied agonist peptides, GLP-1 and exendin-4, share sequence homology at their N-terminal region; however, modifications that can be tolerated here are not fully understood. In this work, a functional screen of GLP-1 variants with randomized N-terminal domains reveals new GLP-1R agonists and uncovers a pattern whereby a negative charge is preferred at the third position in various sequence contexts. We further tested this sequence-structure-activity principle by synthesizing peptide analogues where this position was mutated to both canonical and noncanonical amino acids. We discovered a highly active GLP-1 analogue in which the native glutamate residue three positions from the N-terminus was replaced with the sulfo-containing amino acid cysteic acid (GLP-1-CYA). The receptor binding and downstream signaling properties elicited by GLP-1-CYA were similar to the wild type GLP-1 peptide. Computational modeling identified a likely mode of interaction of the negatively charged side chain in GLP-1-CYA with an arginine on GLP-1R. This work highlights a strategy of combinatorial peptide screening coupled with chemical exploration that could be used to generate novel agonists for other receptors with peptide ligands. 2022-03-23T15:18:59Z 2022-03-15T18:52:29Z 2022-03-23T15:18:59Z 2020-12 2020-09 2022-03-15T18:49:01Z Article http://purl.org/eprint/type/JournalArticle 1554-8929 1554-8937 https://hdl.handle.net/1721.1/141201.2 Longwell, Chelsea K, Hanna, Stephanie, Hartrampf, Nina, Sperberg, R Andres Parra, Huang, Po-Ssu et al. 2021. "Identification of N-Terminally Diversified GLP-1R Agonists Using Saturation Mutagenesis and Chemical Design." ACS Chemical Biology, 16 (1). en http://dx.doi.org/10.1021/acschembio.0c00722 ACS Chemical Biology Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/octet-stream American Chemical Society (ACS) ACS |
| spellingShingle | Longwell, Chelsea K. Hanna, Stephanie Hartrampf, Nina Sperberg, R. Andres Parra Huang, Po-Ssu Pentelute, Bradley L. Cochran, Jennifer R. Identification of N-Terminally Diversified GLP-1R Agonists Using Saturation Mutagenesis and Chemical Design |
| title | Identification of N-Terminally Diversified GLP-1R Agonists Using Saturation Mutagenesis and Chemical Design |
| title_full | Identification of N-Terminally Diversified GLP-1R Agonists Using Saturation Mutagenesis and Chemical Design |
| title_fullStr | Identification of N-Terminally Diversified GLP-1R Agonists Using Saturation Mutagenesis and Chemical Design |
| title_full_unstemmed | Identification of N-Terminally Diversified GLP-1R Agonists Using Saturation Mutagenesis and Chemical Design |
| title_short | Identification of N-Terminally Diversified GLP-1R Agonists Using Saturation Mutagenesis and Chemical Design |
| title_sort | identification of n terminally diversified glp 1r agonists using saturation mutagenesis and chemical design |
| url | https://hdl.handle.net/1721.1/141201.2 |
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