Genetically targeted chemical assembly of functional materials in living cells, tissues, and animals
© 2020 American Association for the Advancement of Science. All rights reserved. The structural and functional complexity of multicellular biological systems, such as the brain, are beyond the reach of human design or assembly capabilities. Cells in living organisms may be recruited to construct syn...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
American Association for the Advancement of Science (AAAS)
2022
|
| Online Access: | https://hdl.handle.net/1721.1/141320 |
| _version_ | 1826197885497114624 |
|---|---|
| author | Liu, Jia Kim, Yoon Seok Richardson, Claire E Tom, Ariane Ramakrishnan, Charu Birey, Fikri Katsumata, Toru Chen, Shucheng Wang, Cheng Wang, Xiao Joubert, Lydia-Marie Jiang, Yuanwen Wang, Huiliang Fenno, Lief E Tok, Jeffrey B-H Pașca, Sergiu P Shen, Kang Bao, Zhenan Deisseroth, Karl |
| author_facet | Liu, Jia Kim, Yoon Seok Richardson, Claire E Tom, Ariane Ramakrishnan, Charu Birey, Fikri Katsumata, Toru Chen, Shucheng Wang, Cheng Wang, Xiao Joubert, Lydia-Marie Jiang, Yuanwen Wang, Huiliang Fenno, Lief E Tok, Jeffrey B-H Pașca, Sergiu P Shen, Kang Bao, Zhenan Deisseroth, Karl |
| author_sort | Liu, Jia |
| collection | MIT |
| description | © 2020 American Association for the Advancement of Science. All rights reserved. The structural and functional complexity of multicellular biological systems, such as the brain, are beyond the reach of human design or assembly capabilities. Cells in living organisms may be recruited to construct synthetic materials or structures if treated as anatomically defined compartments for specific chemistry, harnessing biology for the assembly of complex functional structures. By integrating engineered-enzyme targeting and polymer chemistry, we genetically instructed specific living neurons to guide chemical synthesis of electrically functional (conductive or insulating) polymers at the plasma membrane. Electrophysiological and behavioral analyses confirmed that rationally designed, genetically targeted assembly of functional polymers not only preserved neuronal viability but also achieved remodeling of membrane properties and modulated cell type-specific behaviors in freely moving animals. This approach may enable the creation of diverse, complex, and functional structures and materials within living systems. |
| first_indexed | 2024-09-23T10:55:09Z |
| format | Article |
| id | mit-1721.1/141320 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T10:55:09Z |
| publishDate | 2022 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | dspace |
| spelling | mit-1721.1/1413202022-03-22T03:28:50Z Genetically targeted chemical assembly of functional materials in living cells, tissues, and animals Liu, Jia Kim, Yoon Seok Richardson, Claire E Tom, Ariane Ramakrishnan, Charu Birey, Fikri Katsumata, Toru Chen, Shucheng Wang, Cheng Wang, Xiao Joubert, Lydia-Marie Jiang, Yuanwen Wang, Huiliang Fenno, Lief E Tok, Jeffrey B-H Pașca, Sergiu P Shen, Kang Bao, Zhenan Deisseroth, Karl © 2020 American Association for the Advancement of Science. All rights reserved. The structural and functional complexity of multicellular biological systems, such as the brain, are beyond the reach of human design or assembly capabilities. Cells in living organisms may be recruited to construct synthetic materials or structures if treated as anatomically defined compartments for specific chemistry, harnessing biology for the assembly of complex functional structures. By integrating engineered-enzyme targeting and polymer chemistry, we genetically instructed specific living neurons to guide chemical synthesis of electrically functional (conductive or insulating) polymers at the plasma membrane. Electrophysiological and behavioral analyses confirmed that rationally designed, genetically targeted assembly of functional polymers not only preserved neuronal viability but also achieved remodeling of membrane properties and modulated cell type-specific behaviors in freely moving animals. This approach may enable the creation of diverse, complex, and functional structures and materials within living systems. 2022-03-21T13:52:08Z 2022-03-21T13:52:08Z 2020 2022-03-21T13:35:02Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/141320 Liu, Jia, Kim, Yoon Seok, Richardson, Claire E, Tom, Ariane, Ramakrishnan, Charu et al. 2020. "Genetically targeted chemical assembly of functional materials in living cells, tissues, and animals." Science, 367 (6484). en 10.1126/SCIENCE.AAY4866 Science Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf American Association for the Advancement of Science (AAAS) PMC |
| spellingShingle | Liu, Jia Kim, Yoon Seok Richardson, Claire E Tom, Ariane Ramakrishnan, Charu Birey, Fikri Katsumata, Toru Chen, Shucheng Wang, Cheng Wang, Xiao Joubert, Lydia-Marie Jiang, Yuanwen Wang, Huiliang Fenno, Lief E Tok, Jeffrey B-H Pașca, Sergiu P Shen, Kang Bao, Zhenan Deisseroth, Karl Genetically targeted chemical assembly of functional materials in living cells, tissues, and animals |
| title | Genetically targeted chemical assembly of functional materials in living cells, tissues, and animals |
| title_full | Genetically targeted chemical assembly of functional materials in living cells, tissues, and animals |
| title_fullStr | Genetically targeted chemical assembly of functional materials in living cells, tissues, and animals |
| title_full_unstemmed | Genetically targeted chemical assembly of functional materials in living cells, tissues, and animals |
| title_short | Genetically targeted chemical assembly of functional materials in living cells, tissues, and animals |
| title_sort | genetically targeted chemical assembly of functional materials in living cells tissues and animals |
| url | https://hdl.handle.net/1721.1/141320 |
| work_keys_str_mv | AT liujia geneticallytargetedchemicalassemblyoffunctionalmaterialsinlivingcellstissuesandanimals AT kimyoonseok geneticallytargetedchemicalassemblyoffunctionalmaterialsinlivingcellstissuesandanimals AT richardsonclairee geneticallytargetedchemicalassemblyoffunctionalmaterialsinlivingcellstissuesandanimals AT tomariane geneticallytargetedchemicalassemblyoffunctionalmaterialsinlivingcellstissuesandanimals AT ramakrishnancharu geneticallytargetedchemicalassemblyoffunctionalmaterialsinlivingcellstissuesandanimals AT bireyfikri geneticallytargetedchemicalassemblyoffunctionalmaterialsinlivingcellstissuesandanimals AT katsumatatoru geneticallytargetedchemicalassemblyoffunctionalmaterialsinlivingcellstissuesandanimals AT chenshucheng geneticallytargetedchemicalassemblyoffunctionalmaterialsinlivingcellstissuesandanimals AT wangcheng geneticallytargetedchemicalassemblyoffunctionalmaterialsinlivingcellstissuesandanimals AT wangxiao geneticallytargetedchemicalassemblyoffunctionalmaterialsinlivingcellstissuesandanimals AT joubertlydiamarie geneticallytargetedchemicalassemblyoffunctionalmaterialsinlivingcellstissuesandanimals AT jiangyuanwen geneticallytargetedchemicalassemblyoffunctionalmaterialsinlivingcellstissuesandanimals AT wanghuiliang geneticallytargetedchemicalassemblyoffunctionalmaterialsinlivingcellstissuesandanimals AT fennoliefe geneticallytargetedchemicalassemblyoffunctionalmaterialsinlivingcellstissuesandanimals AT tokjeffreybh geneticallytargetedchemicalassemblyoffunctionalmaterialsinlivingcellstissuesandanimals AT pascasergiup geneticallytargetedchemicalassemblyoffunctionalmaterialsinlivingcellstissuesandanimals AT shenkang geneticallytargetedchemicalassemblyoffunctionalmaterialsinlivingcellstissuesandanimals AT baozhenan geneticallytargetedchemicalassemblyoffunctionalmaterialsinlivingcellstissuesandanimals AT deisserothkarl geneticallytargetedchemicalassemblyoffunctionalmaterialsinlivingcellstissuesandanimals |