Dual modes of CRISPR-associated transposon homing
Tn7-like transposons have co-opted CRISPR systems, including class 1 type I-F, I-B, and class 2 type V-K. Intriguingly, although these CRISPR-associated transposases (CASTs) undergo robust CRISPR RNA (crRNA)-guided transposition, they are almost never found in sites targeted by the crRNAs encoded by...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier BV
2022
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| Online Access: | https://hdl.handle.net/1721.1/142535.2 |
| _version_ | 1811087642114129920 |
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| author | Saito, Makoto Ladha, Alim Strecker, Jonathan Faure, Guilhem Neumann, Edwin Altae-Tran, Han Macrae, Rhiannon K. Zhang, Feng |
| author2 | McGovern Institute for Brain Research at MIT |
| author_facet | McGovern Institute for Brain Research at MIT Saito, Makoto Ladha, Alim Strecker, Jonathan Faure, Guilhem Neumann, Edwin Altae-Tran, Han Macrae, Rhiannon K. Zhang, Feng |
| author_sort | Saito, Makoto |
| collection | MIT |
| description | Tn7-like transposons have co-opted CRISPR systems, including class 1 type I-F, I-B, and class 2 type V-K. Intriguingly, although these CRISPR-associated transposases (CASTs) undergo robust CRISPR RNA (crRNA)-guided transposition, they are almost never found in sites targeted by the crRNAs encoded by the cognate CRISPR array. To understand this paradox, we investigated CAST V-K and I-B systems and found two distinct modes of transposition: (1) crRNA-guided transposition and (2) CRISPR array-independent homing. We show distinct CAST systems utilize different molecular mechanisms to target their homing site. Type V-K CAST systems use a short, delocalized crRNA for RNA-guided homing, whereas type I-B CAST systems, which contain two distinct target selector proteins, use TniQ for RNA-guided DNA transposition and TnsD for homing to an attachment site. These observations illuminate a key step in the life cycle of CAST systems and highlight the diversity of molecular mechanisms mediating transposon homing. |
| first_indexed | 2024-09-23T13:49:42Z |
| format | Article |
| id | mit-1721.1/142535.2 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T13:49:42Z |
| publishDate | 2022 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/142535.22024-03-14T17:38:19Z Dual modes of CRISPR-associated transposon homing Saito, Makoto Ladha, Alim Strecker, Jonathan Faure, Guilhem Neumann, Edwin Altae-Tran, Han Macrae, Rhiannon K. Zhang, Feng McGovern Institute for Brain Research at MIT Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Massachusetts Institute of Technology. Department of Biological Engineering Howard Hughes Medical Institute Tn7-like transposons have co-opted CRISPR systems, including class 1 type I-F, I-B, and class 2 type V-K. Intriguingly, although these CRISPR-associated transposases (CASTs) undergo robust CRISPR RNA (crRNA)-guided transposition, they are almost never found in sites targeted by the crRNAs encoded by the cognate CRISPR array. To understand this paradox, we investigated CAST V-K and I-B systems and found two distinct modes of transposition: (1) crRNA-guided transposition and (2) CRISPR array-independent homing. We show distinct CAST systems utilize different molecular mechanisms to target their homing site. Type V-K CAST systems use a short, delocalized crRNA for RNA-guided homing, whereas type I-B CAST systems, which contain two distinct target selector proteins, use TniQ for RNA-guided DNA transposition and TnsD for homing to an attachment site. These observations illuminate a key step in the life cycle of CAST systems and highlight the diversity of molecular mechanisms mediating transposon homing. 2022-05-16T19:19:43Z 2022-05-16T14:19:09Z 2022-05-16T19:19:43Z 2021-03 2021-01 2022-05-16T13:57:53Z Article http://purl.org/eprint/type/JournalArticle 0092-8674 https://hdl.handle.net/1721.1/142535.2 Saito, Makoto, Ladha, Alim, Strecker, Jonathan, Faure, Guilhem, Neumann, Edwin et al. 2021. "Dual modes of CRISPR-associated transposon homing." Cell, 184 (9). en http://dx.doi.org/10.1016/j.cell.2021.03.006 Cell Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licens http://creativecommons.org/licenses/by-nc-nd/4.0/ application/octet-stream Elsevier BV PMC |
| spellingShingle | Saito, Makoto Ladha, Alim Strecker, Jonathan Faure, Guilhem Neumann, Edwin Altae-Tran, Han Macrae, Rhiannon K. Zhang, Feng Dual modes of CRISPR-associated transposon homing |
| title | Dual modes of CRISPR-associated transposon homing |
| title_full | Dual modes of CRISPR-associated transposon homing |
| title_fullStr | Dual modes of CRISPR-associated transposon homing |
| title_full_unstemmed | Dual modes of CRISPR-associated transposon homing |
| title_short | Dual modes of CRISPR-associated transposon homing |
| title_sort | dual modes of crispr associated transposon homing |
| url | https://hdl.handle.net/1721.1/142535.2 |
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