RNA Pol IV induces antagonistic parent-of-origin effects on Arabidopsis endosperm
<jats:p>Gene expression in endosperm—a seed tissue that mediates transfer of maternal resources to offspring—is under complex epigenetic control. We show here that plant-specific RNA polymerase IV (Pol IV) mediates parental control of endosperm gene expression. Pol IV is required for the produ...
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022
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| Online Access: | https://hdl.handle.net/1721.1/142622 |
| _version_ | 1826192663662034944 |
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| author | Satyaki, Prasad RV Gehring, Mary |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Satyaki, Prasad RV Gehring, Mary |
| author_sort | Satyaki, Prasad RV |
| collection | MIT |
| description | <jats:p>Gene expression in endosperm—a seed tissue that mediates transfer of maternal resources to offspring—is under complex epigenetic control. We show here that plant-specific RNA polymerase IV (Pol IV) mediates parental control of endosperm gene expression. Pol IV is required for the production of small interfering RNAs that typically direct DNA methylation. We compared small RNAs (sRNAs), DNA methylation, and mRNAs in <jats:italic>Arabidopsis thaliana</jats:italic> endosperm from heterozygotes produced by reciprocally crossing wild-type (WT) plants to Pol IV mutants. We find that maternally and paternally acting Pol IV induce distinct effects on endosperm. Loss of maternal or paternal Pol IV impacts sRNAs and DNA methylation at different genomic sites. Strikingly, maternally and paternally acting Pol IV have antagonistic impacts on gene expression at some loci, divergently promoting or repressing endosperm gene expression. Antagonistic parent-of-origin effects have only rarely been described and are consistent with a gene regulatory system evolving under parental conflict.</jats:p> |
| first_indexed | 2024-09-23T09:27:23Z |
| format | Article |
| id | mit-1721.1/142622 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T09:27:23Z |
| publishDate | 2022 |
| publisher | Public Library of Science (PLoS) |
| record_format | dspace |
| spelling | mit-1721.1/1426222023-04-20T14:32:22Z RNA Pol IV induces antagonistic parent-of-origin effects on Arabidopsis endosperm Satyaki, Prasad RV Gehring, Mary Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research <jats:p>Gene expression in endosperm—a seed tissue that mediates transfer of maternal resources to offspring—is under complex epigenetic control. We show here that plant-specific RNA polymerase IV (Pol IV) mediates parental control of endosperm gene expression. Pol IV is required for the production of small interfering RNAs that typically direct DNA methylation. We compared small RNAs (sRNAs), DNA methylation, and mRNAs in <jats:italic>Arabidopsis thaliana</jats:italic> endosperm from heterozygotes produced by reciprocally crossing wild-type (WT) plants to Pol IV mutants. We find that maternally and paternally acting Pol IV induce distinct effects on endosperm. Loss of maternal or paternal Pol IV impacts sRNAs and DNA methylation at different genomic sites. Strikingly, maternally and paternally acting Pol IV have antagonistic impacts on gene expression at some loci, divergently promoting or repressing endosperm gene expression. Antagonistic parent-of-origin effects have only rarely been described and are consistent with a gene regulatory system evolving under parental conflict.</jats:p> 2022-05-20T11:56:24Z 2022-05-20T11:56:24Z 2022-04 2022-05-20T11:45:46Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/142622 Satyaki, Prasad RV and Gehring, Mary. 2022. "RNA Pol IV induces antagonistic parent-of-origin effects on Arabidopsis endosperm." PLOS Biology, 20 (4). en 10.1371/journal.pbio.3001602 PLOS Biology Creative Commons Attribution 4.0 International License https://creativecommons.org/licenses/by/4.0 application/pdf Public Library of Science (PLoS) PLoS |
| spellingShingle | Satyaki, Prasad RV Gehring, Mary RNA Pol IV induces antagonistic parent-of-origin effects on Arabidopsis endosperm |
| title | RNA Pol IV induces antagonistic parent-of-origin effects on Arabidopsis endosperm |
| title_full | RNA Pol IV induces antagonistic parent-of-origin effects on Arabidopsis endosperm |
| title_fullStr | RNA Pol IV induces antagonistic parent-of-origin effects on Arabidopsis endosperm |
| title_full_unstemmed | RNA Pol IV induces antagonistic parent-of-origin effects on Arabidopsis endosperm |
| title_short | RNA Pol IV induces antagonistic parent-of-origin effects on Arabidopsis endosperm |
| title_sort | rna pol iv induces antagonistic parent of origin effects on arabidopsis endosperm |
| url | https://hdl.handle.net/1721.1/142622 |
| work_keys_str_mv | AT satyakiprasadrv rnapolivinducesantagonisticparentoforigineffectsonarabidopsisendosperm AT gehringmary rnapolivinducesantagonisticparentoforigineffectsonarabidopsisendosperm |