Placental MRI: Development of an MRI compatible ex vivo system for whole placenta dual perfusion
© 2020 Elsevier Ltd Purpose: Placental dysfunction plays a key role in diseases that affect the fetus in utero and after birth. Aiming to develop a platform for validating in vivo placental MRI and investigations into placental physiology, we designed and built a prototype MRI-compatible perfusion c...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Elsevier BV
2022
|
| Online Access: | https://hdl.handle.net/1721.1/142672 |
| _version_ | 1826204342213935104 |
|---|---|
| author | Stout, Jeffrey N Rouhani, Shahin Turk, Esra Abaci Ha, Christopher G Luo, Jie Rich, Karen Wald, Lawerence L Adalsteinsson, Elfar Barth, William H Grant, P Ellen Roberts, Drucilla J |
| author2 | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science |
| author_facet | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Stout, Jeffrey N Rouhani, Shahin Turk, Esra Abaci Ha, Christopher G Luo, Jie Rich, Karen Wald, Lawerence L Adalsteinsson, Elfar Barth, William H Grant, P Ellen Roberts, Drucilla J |
| author_sort | Stout, Jeffrey N |
| collection | MIT |
| description | © 2020 Elsevier Ltd Purpose: Placental dysfunction plays a key role in diseases that affect the fetus in utero and after birth. Aiming to develop a platform for validating in vivo placental MRI and investigations into placental physiology, we designed and built a prototype MRI-compatible perfusion chamber with an integrated MRI receive coil for high SNR ex vivo placental imaging. Principal results: After optimizing placenta vascular clearing and perfusion protocols, we performed contrast enhanced MR angiography and MR relaxometry on eight carefully selected placentas while they were perfused via the umbilical arteries (UAs). Additionally, two of these placentas underwent maternal perfusion via the intervillous space (IVS). Despite striving for homogenous perfusion across the whole placenta, imaging results were highly heterogeneous for both UA and IVS perfused placentas. By histology, we observed blood congestion in the villi in regions that showed low UA perfusion during MRI. In two placentas prominent chorionic arteries followed by adjacent veins underwent contrast enhancement in the absence of villous capillary blush. The single placenta from a pregnancy affected by IUGR had the most homogeneous villous capillary perfusion. Major conclusions: A dual perfusion system for ex vivo placentas compatible with MRI permitted assessment of UA and IVS placental perfusion. We observed spatial UA perfusion heterogeneity and evidence for arteriovenous shunting in placentas from normal pregnancies and deliveries, but relative villous capillary perfusion homogeneity in a single IUGR placenta. Future work will focus on system optimization, followed by physiological manipulation and validation of in vivo placental MRI. |
| first_indexed | 2024-09-23T12:53:08Z |
| format | Article |
| id | mit-1721.1/142672 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T12:53:08Z |
| publishDate | 2022 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1426722024-03-19T13:45:27Z Placental MRI: Development of an MRI compatible ex vivo system for whole placenta dual perfusion Stout, Jeffrey N Rouhani, Shahin Turk, Esra Abaci Ha, Christopher G Luo, Jie Rich, Karen Wald, Lawerence L Adalsteinsson, Elfar Barth, William H Grant, P Ellen Roberts, Drucilla J Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Institute for Medical Engineering & Science © 2020 Elsevier Ltd Purpose: Placental dysfunction plays a key role in diseases that affect the fetus in utero and after birth. Aiming to develop a platform for validating in vivo placental MRI and investigations into placental physiology, we designed and built a prototype MRI-compatible perfusion chamber with an integrated MRI receive coil for high SNR ex vivo placental imaging. Principal results: After optimizing placenta vascular clearing and perfusion protocols, we performed contrast enhanced MR angiography and MR relaxometry on eight carefully selected placentas while they were perfused via the umbilical arteries (UAs). Additionally, two of these placentas underwent maternal perfusion via the intervillous space (IVS). Despite striving for homogenous perfusion across the whole placenta, imaging results were highly heterogeneous for both UA and IVS perfused placentas. By histology, we observed blood congestion in the villi in regions that showed low UA perfusion during MRI. In two placentas prominent chorionic arteries followed by adjacent veins underwent contrast enhancement in the absence of villous capillary blush. The single placenta from a pregnancy affected by IUGR had the most homogeneous villous capillary perfusion. Major conclusions: A dual perfusion system for ex vivo placentas compatible with MRI permitted assessment of UA and IVS placental perfusion. We observed spatial UA perfusion heterogeneity and evidence for arteriovenous shunting in placentas from normal pregnancies and deliveries, but relative villous capillary perfusion homogeneity in a single IUGR placenta. Future work will focus on system optimization, followed by physiological manipulation and validation of in vivo placental MRI. 2022-05-24T14:33:15Z 2022-05-24T14:33:15Z 2020 2022-05-24T14:19:43Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/142672 Stout, Jeffrey N, Rouhani, Shahin, Turk, Esra Abaci, Ha, Christopher G, Luo, Jie et al. 2020. "Placental MRI: Development of an MRI compatible ex vivo system for whole placenta dual perfusion." Placenta, 101. en 10.1016/J.PLACENTA.2020.07.026 Placenta Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licens http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier BV Elsevier |
| spellingShingle | Stout, Jeffrey N Rouhani, Shahin Turk, Esra Abaci Ha, Christopher G Luo, Jie Rich, Karen Wald, Lawerence L Adalsteinsson, Elfar Barth, William H Grant, P Ellen Roberts, Drucilla J Placental MRI: Development of an MRI compatible ex vivo system for whole placenta dual perfusion |
| title | Placental MRI: Development of an MRI compatible ex vivo system for whole placenta dual perfusion |
| title_full | Placental MRI: Development of an MRI compatible ex vivo system for whole placenta dual perfusion |
| title_fullStr | Placental MRI: Development of an MRI compatible ex vivo system for whole placenta dual perfusion |
| title_full_unstemmed | Placental MRI: Development of an MRI compatible ex vivo system for whole placenta dual perfusion |
| title_short | Placental MRI: Development of an MRI compatible ex vivo system for whole placenta dual perfusion |
| title_sort | placental mri development of an mri compatible ex vivo system for whole placenta dual perfusion |
| url | https://hdl.handle.net/1721.1/142672 |
| work_keys_str_mv | AT stoutjeffreyn placentalmridevelopmentofanmricompatibleexvivosystemforwholeplacentadualperfusion AT rouhanishahin placentalmridevelopmentofanmricompatibleexvivosystemforwholeplacentadualperfusion AT turkesraabaci placentalmridevelopmentofanmricompatibleexvivosystemforwholeplacentadualperfusion AT hachristopherg placentalmridevelopmentofanmricompatibleexvivosystemforwholeplacentadualperfusion AT luojie placentalmridevelopmentofanmricompatibleexvivosystemforwholeplacentadualperfusion AT richkaren placentalmridevelopmentofanmricompatibleexvivosystemforwholeplacentadualperfusion AT waldlawerencel placentalmridevelopmentofanmricompatibleexvivosystemforwholeplacentadualperfusion AT adalsteinssonelfar placentalmridevelopmentofanmricompatibleexvivosystemforwholeplacentadualperfusion AT barthwilliamh placentalmridevelopmentofanmricompatibleexvivosystemforwholeplacentadualperfusion AT grantpellen placentalmridevelopmentofanmricompatibleexvivosystemforwholeplacentadualperfusion AT robertsdrucillaj placentalmridevelopmentofanmricompatibleexvivosystemforwholeplacentadualperfusion |