Gene expression changes during mammalian male meiotic initiation
In sexually reproducing organisms, germ cells can divide by mitosis, which generates daughter cells that are genetically identical to the mother cell, or meiosis, which divides the genome for haploid gamete generation. Germ cells undergo meiotic initiation, the transition from the mitotic cell cycle...
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| Format: | Thesis |
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Massachusetts Institute of Technology
2022
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| Online Access: | https://hdl.handle.net/1721.1/142698 |
| _version_ | 1826213287929315328 |
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| author | Christensen, Holly C. |
| author2 | Page, David C. |
| author_facet | Page, David C. Christensen, Holly C. |
| author_sort | Christensen, Holly C. |
| collection | MIT |
| description | In sexually reproducing organisms, germ cells can divide by mitosis, which generates daughter cells that are genetically identical to the mother cell, or meiosis, which divides the genome for haploid gamete generation. Germ cells undergo meiotic initiation, the transition from the mitotic cell cycle to the meiotic cell cycle, once in a generation. In mammals, genetic studies have identified key regulators of meiotic initiation. However, a holistic assessment of the transcriptional changes during meiotic initiation has not been performed. In this thesis, we describe the transcriptional changes that occur during meiotic initiation in male mice.
Previously, obtaining pure populations of male germ cells right before, during, and immediately after meiotic initiation was not experimentally achievable. Using the 3S method, which combines spermatogenesis synchronization, lineage tracing, and cell sorting, we obtained pure populations of undifferentiated Type A spermatogonia, Type B spermatogonia, STRA8- preleptotene spermatocytes, loSTRA8 preleptotene spermatocytes, hiSTRA8 preleptotene spermatocytes, and leptotene spermatocytes. Following transcriptome sequencing, data analysis showed several different patterns of gene expression during meiotic initiation, but the largest groups of genes showed either increased or decreased expression across the time course. While the genes whose expression increased at meiotic initiation were enriched for functions in meiotic prophase I, the genes whose expression decreased at meiotic initiation were enriched for housekeeping processes, including mRNA and protein regulation. Both upregulated and downregulated genes were expressed throughout the time course, but their expression levels were modulated at meiotic initiation. By performing motif enrichment analysis at the proximal promoters, we identified YY1 as a potential regulator of the genes whose expression decreases at meiotic initiation. In taking a descriptive experimental approach we identified novel gene expression changes during meiotic initiation, which furthers our understanding of germ cell biology. |
| first_indexed | 2024-09-23T15:46:41Z |
| format | Thesis |
| id | mit-1721.1/142698 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T15:46:41Z |
| publishDate | 2022 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/1426982022-05-25T03:05:16Z Gene expression changes during mammalian male meiotic initiation Christensen, Holly C. Page, David C. Massachusetts Institute of Technology. Department of Biology In sexually reproducing organisms, germ cells can divide by mitosis, which generates daughter cells that are genetically identical to the mother cell, or meiosis, which divides the genome for haploid gamete generation. Germ cells undergo meiotic initiation, the transition from the mitotic cell cycle to the meiotic cell cycle, once in a generation. In mammals, genetic studies have identified key regulators of meiotic initiation. However, a holistic assessment of the transcriptional changes during meiotic initiation has not been performed. In this thesis, we describe the transcriptional changes that occur during meiotic initiation in male mice. Previously, obtaining pure populations of male germ cells right before, during, and immediately after meiotic initiation was not experimentally achievable. Using the 3S method, which combines spermatogenesis synchronization, lineage tracing, and cell sorting, we obtained pure populations of undifferentiated Type A spermatogonia, Type B spermatogonia, STRA8- preleptotene spermatocytes, loSTRA8 preleptotene spermatocytes, hiSTRA8 preleptotene spermatocytes, and leptotene spermatocytes. Following transcriptome sequencing, data analysis showed several different patterns of gene expression during meiotic initiation, but the largest groups of genes showed either increased or decreased expression across the time course. While the genes whose expression increased at meiotic initiation were enriched for functions in meiotic prophase I, the genes whose expression decreased at meiotic initiation were enriched for housekeeping processes, including mRNA and protein regulation. Both upregulated and downregulated genes were expressed throughout the time course, but their expression levels were modulated at meiotic initiation. By performing motif enrichment analysis at the proximal promoters, we identified YY1 as a potential regulator of the genes whose expression decreases at meiotic initiation. In taking a descriptive experimental approach we identified novel gene expression changes during meiotic initiation, which furthers our understanding of germ cell biology. Ph.D. 2022-05-24T19:19:42Z 2022-05-24T19:19:42Z 2021-09 2022-05-19T19:26:10.169Z Thesis https://hdl.handle.net/1721.1/142698 In Copyright - Educational Use Permitted Copyright retained by author(s) https://rightsstatements.org/page/InC-EDU/1.0/ application/pdf Massachusetts Institute of Technology |
| spellingShingle | Christensen, Holly C. Gene expression changes during mammalian male meiotic initiation |
| title | Gene expression changes during mammalian male meiotic initiation |
| title_full | Gene expression changes during mammalian male meiotic initiation |
| title_fullStr | Gene expression changes during mammalian male meiotic initiation |
| title_full_unstemmed | Gene expression changes during mammalian male meiotic initiation |
| title_short | Gene expression changes during mammalian male meiotic initiation |
| title_sort | gene expression changes during mammalian male meiotic initiation |
| url | https://hdl.handle.net/1721.1/142698 |
| work_keys_str_mv | AT christensenhollyc geneexpressionchangesduringmammalianmalemeioticinitiation |