A Robust Method for Perfusable Microvascular Network Formation In Vitro

Micropost-based microfluidic devices are widely used for microvascular network (MVN) formation in diverse research fields. However, consistently generating perfusable MVNs of physiological morphology and dimension has proven to be challenging. Here, how initial seeding parameters determine key chara...

Full description

Bibliographic Details
Main Authors: Wan, Zhengpeng, Zhong, Amy X, Zhang, Shun, Pavlou, Georgios, Coughlin, Mark F, Shelton, Sarah E, Nguyen, Huu Tuan, Lorch, Jochen H, Barbie, David A, Kamm, Roger D
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering
Format: Article
Language:English
Published: Wiley 2022
Online Access:https://hdl.handle.net/1721.1/142858
Description
Summary:Micropost-based microfluidic devices are widely used for microvascular network (MVN) formation in diverse research fields. However, consistently generating perfusable MVNs of physiological morphology and dimension has proven to be challenging. Here, how initial seeding parameters determine key characteristics of MVN formation is investigated and a robust two-step seeding strategy to generate perfusable physiological MVNs in microfluidic devices is established.