A Robust Method for Perfusable Microvascular Network Formation In Vitro
Micropost-based microfluidic devices are widely used for microvascular network (MVN) formation in diverse research fields. However, consistently generating perfusable MVNs of physiological morphology and dimension has proven to be challenging. Here, how initial seeding parameters determine key chara...
| Main Authors: | , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022
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| Online Access: | https://hdl.handle.net/1721.1/142858 |
| _version_ | 1826191546626605056 |
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| author | Wan, Zhengpeng Zhong, Amy X Zhang, Shun Pavlou, Georgios Coughlin, Mark F Shelton, Sarah E Nguyen, Huu Tuan Lorch, Jochen H Barbie, David A Kamm, Roger D |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Wan, Zhengpeng Zhong, Amy X Zhang, Shun Pavlou, Georgios Coughlin, Mark F Shelton, Sarah E Nguyen, Huu Tuan Lorch, Jochen H Barbie, David A Kamm, Roger D |
| author_sort | Wan, Zhengpeng |
| collection | MIT |
| description | Micropost-based microfluidic devices are widely used for microvascular network (MVN) formation in diverse research fields. However, consistently generating perfusable MVNs of physiological morphology and dimension has proven to be challenging. Here, how initial seeding parameters determine key characteristics of MVN formation is investigated and a robust two-step seeding strategy to generate perfusable physiological MVNs in microfluidic devices is established. |
| first_indexed | 2024-09-23T08:57:33Z |
| format | Article |
| id | mit-1721.1/142858 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:57:33Z |
| publishDate | 2022 |
| publisher | Wiley |
| record_format | dspace |
| spelling | mit-1721.1/1428582023-01-30T20:37:33Z A Robust Method for Perfusable Microvascular Network Formation In Vitro Wan, Zhengpeng Zhong, Amy X Zhang, Shun Pavlou, Georgios Coughlin, Mark F Shelton, Sarah E Nguyen, Huu Tuan Lorch, Jochen H Barbie, David A Kamm, Roger D Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Micropost-based microfluidic devices are widely used for microvascular network (MVN) formation in diverse research fields. However, consistently generating perfusable MVNs of physiological morphology and dimension has proven to be challenging. Here, how initial seeding parameters determine key characteristics of MVN formation is investigated and a robust two-step seeding strategy to generate perfusable physiological MVNs in microfluidic devices is established. 2022-06-01T19:32:15Z 2022-06-01T19:32:15Z 2022-04-03 2022-06-01T19:21:08Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/142858 Wan, Zhengpeng, Zhong, Amy X, Zhang, Shun, Pavlou, Georgios, Coughlin, Mark F et al. 2022. "A Robust Method for Perfusable Microvascular Network Formation In Vitro." Small Methods. en 10.1002/smtd.202200143 Small Methods Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licens http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Wiley Wiley |
| spellingShingle | Wan, Zhengpeng Zhong, Amy X Zhang, Shun Pavlou, Georgios Coughlin, Mark F Shelton, Sarah E Nguyen, Huu Tuan Lorch, Jochen H Barbie, David A Kamm, Roger D A Robust Method for Perfusable Microvascular Network Formation In Vitro |
| title | A Robust Method for Perfusable Microvascular Network Formation In Vitro |
| title_full | A Robust Method for Perfusable Microvascular Network Formation In Vitro |
| title_fullStr | A Robust Method for Perfusable Microvascular Network Formation In Vitro |
| title_full_unstemmed | A Robust Method for Perfusable Microvascular Network Formation In Vitro |
| title_short | A Robust Method for Perfusable Microvascular Network Formation In Vitro |
| title_sort | robust method for perfusable microvascular network formation in vitro |
| url | https://hdl.handle.net/1721.1/142858 |
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