A cellular and spatial map of the choroid plexus across brain ventricles and ages
The choroid plexus (ChP) in each brain ventricle produces cerebrospinal fluid (CSF) and forms the blood-CSF barrier. Here, we construct a single-cell and spatial atlas of each ChP in the developing, adult, and aged mouse brain. We delineate diverse cell types, subtypes, cell states, and expression p...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier BV
2022
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| Online Access: | https://hdl.handle.net/1721.1/143444 |
| _version_ | 1826209814266511360 |
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| author | Dani, Neil Herbst, Rebecca H McCabe, Cristin Green, Gilad S Kaiser, Karol Head, Joshua P Cui, Jin Shipley, Frederick B Jang, Ahram Dionne, Danielle Nguyen, Lan Rodman, Christopher Riesenfeld, Samantha J Prochazka, Jan Prochazkova, Michaela Sedlacek, Radislav Zhang, Feng Bryja, Vitezslav Rozenblatt-Rosen, Orit Habib, Naomi Regev, Aviv Lehtinen, Maria K |
| author2 | McGovern Institute for Brain Research at MIT |
| author_facet | McGovern Institute for Brain Research at MIT Dani, Neil Herbst, Rebecca H McCabe, Cristin Green, Gilad S Kaiser, Karol Head, Joshua P Cui, Jin Shipley, Frederick B Jang, Ahram Dionne, Danielle Nguyen, Lan Rodman, Christopher Riesenfeld, Samantha J Prochazka, Jan Prochazkova, Michaela Sedlacek, Radislav Zhang, Feng Bryja, Vitezslav Rozenblatt-Rosen, Orit Habib, Naomi Regev, Aviv Lehtinen, Maria K |
| author_sort | Dani, Neil |
| collection | MIT |
| description | The choroid plexus (ChP) in each brain ventricle produces cerebrospinal fluid (CSF) and forms the blood-CSF barrier. Here, we construct a single-cell and spatial atlas of each ChP in the developing, adult, and aged mouse brain. We delineate diverse cell types, subtypes, cell states, and expression programs in epithelial and mesenchymal cells across ages and ventricles. In the developing ChP, we predict a common progenitor pool for epithelial and neuronal cells, validated by lineage tracing. Epithelial and fibroblast cells show regionalized expression by ventricle, starting at embryonic stages and persisting with age, with a dramatic transcriptional shift with maturation, and a smaller shift in each aged cell type. With aging, epithelial cells upregulate host-defense programs, and resident macrophages upregulate interleukin-1β (IL-1β) signaling genes. Our atlas reveals cellular diversity, architecture and signaling across ventricles during development, maturation, and aging of the ChP-brain barrier. |
| first_indexed | 2024-09-23T14:31:17Z |
| format | Article |
| id | mit-1721.1/143444 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T14:31:17Z |
| publishDate | 2022 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1434442023-12-08T17:53:34Z A cellular and spatial map of the choroid plexus across brain ventricles and ages Dani, Neil Herbst, Rebecca H McCabe, Cristin Green, Gilad S Kaiser, Karol Head, Joshua P Cui, Jin Shipley, Frederick B Jang, Ahram Dionne, Danielle Nguyen, Lan Rodman, Christopher Riesenfeld, Samantha J Prochazka, Jan Prochazkova, Michaela Sedlacek, Radislav Zhang, Feng Bryja, Vitezslav Rozenblatt-Rosen, Orit Habib, Naomi Regev, Aviv Lehtinen, Maria K McGovern Institute for Brain Research at MIT Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Massachusetts Institute of Technology. Department of Biological Engineering Koch Institute for Integrative Cancer Research at MIT Massachusetts Institute of Technology. Department of Biology The choroid plexus (ChP) in each brain ventricle produces cerebrospinal fluid (CSF) and forms the blood-CSF barrier. Here, we construct a single-cell and spatial atlas of each ChP in the developing, adult, and aged mouse brain. We delineate diverse cell types, subtypes, cell states, and expression programs in epithelial and mesenchymal cells across ages and ventricles. In the developing ChP, we predict a common progenitor pool for epithelial and neuronal cells, validated by lineage tracing. Epithelial and fibroblast cells show regionalized expression by ventricle, starting at embryonic stages and persisting with age, with a dramatic transcriptional shift with maturation, and a smaller shift in each aged cell type. With aging, epithelial cells upregulate host-defense programs, and resident macrophages upregulate interleukin-1β (IL-1β) signaling genes. Our atlas reveals cellular diversity, architecture and signaling across ventricles during development, maturation, and aging of the ChP-brain barrier. 2022-06-15T16:55:31Z 2022-06-15T16:55:31Z 2021 2022-06-15T16:51:58Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/143444 Dani, Neil, Herbst, Rebecca H, McCabe, Cristin, Green, Gilad S, Kaiser, Karol et al. 2021. "A cellular and spatial map of the choroid plexus across brain ventricles and ages." Cell, 184 (11). en 10.1016/J.CELL.2021.04.003 Cell Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier BV PMC |
| spellingShingle | Dani, Neil Herbst, Rebecca H McCabe, Cristin Green, Gilad S Kaiser, Karol Head, Joshua P Cui, Jin Shipley, Frederick B Jang, Ahram Dionne, Danielle Nguyen, Lan Rodman, Christopher Riesenfeld, Samantha J Prochazka, Jan Prochazkova, Michaela Sedlacek, Radislav Zhang, Feng Bryja, Vitezslav Rozenblatt-Rosen, Orit Habib, Naomi Regev, Aviv Lehtinen, Maria K A cellular and spatial map of the choroid plexus across brain ventricles and ages |
| title | A cellular and spatial map of the choroid plexus across brain ventricles and ages |
| title_full | A cellular and spatial map of the choroid plexus across brain ventricles and ages |
| title_fullStr | A cellular and spatial map of the choroid plexus across brain ventricles and ages |
| title_full_unstemmed | A cellular and spatial map of the choroid plexus across brain ventricles and ages |
| title_short | A cellular and spatial map of the choroid plexus across brain ventricles and ages |
| title_sort | cellular and spatial map of the choroid plexus across brain ventricles and ages |
| url | https://hdl.handle.net/1721.1/143444 |
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