MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum
Comet assay is a standard approach for studying DNA damage in malaria, but high-throughput options are not available. The CometChip was previously developed using mammalian cells as a high-throughput version of the comet assay. It is based on the same principle as the comet assay but provides greate...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
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Elsevier BV
2022
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| Online Access: | https://hdl.handle.net/1721.1/143612 |
| _version_ | 1811077903337652224 |
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| author | Xiong, Aoli Kaushal, Simran Tay, Ian Junjie Engelward, Bevin P Han, Jongyoon Preiser, Peter R |
| author2 | Singapore-MIT Alliance in Research and Technology (SMART) |
| author_facet | Singapore-MIT Alliance in Research and Technology (SMART) Xiong, Aoli Kaushal, Simran Tay, Ian Junjie Engelward, Bevin P Han, Jongyoon Preiser, Peter R |
| author_sort | Xiong, Aoli |
| collection | MIT |
| description | Comet assay is a standard approach for studying DNA damage in malaria, but high-throughput options are not available. The CometChip was previously developed using mammalian cells as a high-throughput version of the comet assay. It is based on the same principle as the comet assay but provides greater efficacy, automated data processing, and improved consistency between experiments. In this protocol, we present MalariaCometChip to quantitatively assess drug-induced DNA damage in Plasmodium falciparum. For complete details on the use and execution of this protocol, please refer to Xiong et al. (2020). |
| first_indexed | 2024-09-23T10:50:07Z |
| format | Article |
| id | mit-1721.1/143612 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T10:50:07Z |
| publishDate | 2022 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1436122023-02-09T15:41:21Z MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum Xiong, Aoli Kaushal, Simran Tay, Ian Junjie Engelward, Bevin P Han, Jongyoon Preiser, Peter R Singapore-MIT Alliance in Research and Technology (SMART) Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Comet assay is a standard approach for studying DNA damage in malaria, but high-throughput options are not available. The CometChip was previously developed using mammalian cells as a high-throughput version of the comet assay. It is based on the same principle as the comet assay but provides greater efficacy, automated data processing, and improved consistency between experiments. In this protocol, we present MalariaCometChip to quantitatively assess drug-induced DNA damage in Plasmodium falciparum. For complete details on the use and execution of this protocol, please refer to Xiong et al. (2020). 2022-06-30T16:19:09Z 2022-06-30T16:19:09Z 2021 2022-06-30T15:52:33Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/143612 Xiong, Aoli, Kaushal, Simran, Tay, Ian Junjie, Engelward, Bevin P, Han, Jongyoon et al. 2021. "MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum." STAR Protocols, 2 (3). en 10.1016/J.XPRO.2021.100797 STAR Protocols Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier BV Elsevier |
| spellingShingle | Xiong, Aoli Kaushal, Simran Tay, Ian Junjie Engelward, Bevin P Han, Jongyoon Preiser, Peter R MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum |
| title | MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum |
| title_full | MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum |
| title_fullStr | MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum |
| title_full_unstemmed | MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum |
| title_short | MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum |
| title_sort | malariacometchip for high throughput quantification of dna damage in plasmodium falciparum |
| url | https://hdl.handle.net/1721.1/143612 |
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