Compact RNA editors with small Cas13 proteins
CRISPR-Cas13 systems have been developed for precise RNA editing, and can potentially be used therapeutically when temporary changes are desirable or when DNA editing is challenging. We have identified and characterized an ultrasmall family of Cas13b proteins-Cas13bt-that can mediate mammalian trans...
| Main Authors: | , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
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Springer Science and Business Media LLC
2022
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| Online Access: | https://hdl.handle.net/1721.1/144412 |
| _version_ | 1811095647393153024 |
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| author | Kannan, Soumya Altae-Tran, Han Jin, Xin Madigan, Victoria J Oshiro, Rachel Makarova, Kira S Koonin, Eugene V Zhang, Feng |
| author2 | Howard Hughes Medical Institute |
| author_facet | Howard Hughes Medical Institute Kannan, Soumya Altae-Tran, Han Jin, Xin Madigan, Victoria J Oshiro, Rachel Makarova, Kira S Koonin, Eugene V Zhang, Feng |
| author_sort | Kannan, Soumya |
| collection | MIT |
| description | CRISPR-Cas13 systems have been developed for precise RNA editing, and can potentially be used therapeutically when temporary changes are desirable or when DNA editing is challenging. We have identified and characterized an ultrasmall family of Cas13b proteins-Cas13bt-that can mediate mammalian transcript knockdown. We have engineered compact variants of REPAIR and RESCUE RNA editors by functionalizing Cas13bt with adenosine and cytosine deaminase domains, and demonstrated packaging of the editors within a single adeno-associated virus. |
| first_indexed | 2024-09-23T16:22:41Z |
| format | Article |
| id | mit-1721.1/144412 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T16:22:41Z |
| publishDate | 2022 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1444122023-01-17T20:35:04Z Compact RNA editors with small Cas13 proteins Kannan, Soumya Altae-Tran, Han Jin, Xin Madigan, Victoria J Oshiro, Rachel Makarova, Kira S Koonin, Eugene V Zhang, Feng Howard Hughes Medical Institute McGovern Institute for Brain Research at MIT Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences CRISPR-Cas13 systems have been developed for precise RNA editing, and can potentially be used therapeutically when temporary changes are desirable or when DNA editing is challenging. We have identified and characterized an ultrasmall family of Cas13b proteins-Cas13bt-that can mediate mammalian transcript knockdown. We have engineered compact variants of REPAIR and RESCUE RNA editors by functionalizing Cas13bt with adenosine and cytosine deaminase domains, and demonstrated packaging of the editors within a single adeno-associated virus. 2022-08-22T16:54:47Z 2022-08-22T16:54:47Z 2022-02 2022-08-22T16:43:45Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/144412 Kannan, Soumya, Altae-Tran, Han, Jin, Xin, Madigan, Victoria J, Oshiro, Rachel et al. 2022. "Compact RNA editors with small Cas13 proteins." Nature Biotechnology, 40 (2). en 10.1038/s41587-021-01030-2 Nature Biotechnology Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Springer Science and Business Media LLC PMC |
| spellingShingle | Kannan, Soumya Altae-Tran, Han Jin, Xin Madigan, Victoria J Oshiro, Rachel Makarova, Kira S Koonin, Eugene V Zhang, Feng Compact RNA editors with small Cas13 proteins |
| title | Compact RNA editors with small Cas13 proteins |
| title_full | Compact RNA editors with small Cas13 proteins |
| title_fullStr | Compact RNA editors with small Cas13 proteins |
| title_full_unstemmed | Compact RNA editors with small Cas13 proteins |
| title_short | Compact RNA editors with small Cas13 proteins |
| title_sort | compact rna editors with small cas13 proteins |
| url | https://hdl.handle.net/1721.1/144412 |
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