Activation of a signaling pathway by the physical translocation of a chromosome
In every organism, the cell cycle requires the execution of multiple processes in a strictly defined order. However, the mechanisms used to ensure such order remain poorly understood, particularly in bacteria. Here, we show that the activation of the essential CtrA signaling pathway that triggers ce...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Elsevier BV
2022
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| Online Access: | https://hdl.handle.net/1721.1/144414 |
| _version_ | 1826190405609193472 |
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| author | Guzzo, Mathilde Sanderlin, Allen G Castro, Lennice K Laub, Michael T |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Guzzo, Mathilde Sanderlin, Allen G Castro, Lennice K Laub, Michael T |
| author_sort | Guzzo, Mathilde |
| collection | MIT |
| description | In every organism, the cell cycle requires the execution of multiple processes in a strictly defined order. However, the mechanisms used to ensure such order remain poorly understood, particularly in bacteria. Here, we show that the activation of the essential CtrA signaling pathway that triggers cell division in Caulobacter crescentus is intrinsically coupled to the initiation of DNA replication via the physical translocation of a newly replicated chromosome, powered by the ParABS system. We demonstrate that ParA accumulation at the new cell pole during chromosome segregation recruits ChpT, an intermediate component of the CtrA signaling pathway. ChpT is normally restricted from accessing the selective PopZ polar microdomain until the new chromosome and ParA arrive. Consequently, any disruption to DNA replication initiation prevents ChpT polarization and, in turn, cell division. Collectively, our findings reveal how major cell-cycle events are coordinated in Caulobacter and, importantly, how chromosome translocation triggers an essential signaling pathway. |
| first_indexed | 2024-09-23T08:39:46Z |
| format | Article |
| id | mit-1721.1/144414 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:39:46Z |
| publishDate | 2022 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1444142023-07-10T19:28:31Z Activation of a signaling pathway by the physical translocation of a chromosome Guzzo, Mathilde Sanderlin, Allen G Castro, Lennice K Laub, Michael T Massachusetts Institute of Technology. Department of Biology Howard Hughes Medical Institute In every organism, the cell cycle requires the execution of multiple processes in a strictly defined order. However, the mechanisms used to ensure such order remain poorly understood, particularly in bacteria. Here, we show that the activation of the essential CtrA signaling pathway that triggers cell division in Caulobacter crescentus is intrinsically coupled to the initiation of DNA replication via the physical translocation of a newly replicated chromosome, powered by the ParABS system. We demonstrate that ParA accumulation at the new cell pole during chromosome segregation recruits ChpT, an intermediate component of the CtrA signaling pathway. ChpT is normally restricted from accessing the selective PopZ polar microdomain until the new chromosome and ParA arrive. Consequently, any disruption to DNA replication initiation prevents ChpT polarization and, in turn, cell division. Collectively, our findings reveal how major cell-cycle events are coordinated in Caulobacter and, importantly, how chromosome translocation triggers an essential signaling pathway. 2022-08-22T17:29:05Z 2022-08-22T17:29:05Z 2021 2022-08-22T16:59:02Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/144414 Guzzo, Mathilde, Sanderlin, Allen G, Castro, Lennice K and Laub, Michael T. 2021. "Activation of a signaling pathway by the physical translocation of a chromosome." Developmental Cell, 56 (15). en 10.1016/J.DEVCEL.2021.06.014 Developmental Cell Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier BV PMC |
| spellingShingle | Guzzo, Mathilde Sanderlin, Allen G Castro, Lennice K Laub, Michael T Activation of a signaling pathway by the physical translocation of a chromosome |
| title | Activation of a signaling pathway by the physical translocation of a chromosome |
| title_full | Activation of a signaling pathway by the physical translocation of a chromosome |
| title_fullStr | Activation of a signaling pathway by the physical translocation of a chromosome |
| title_full_unstemmed | Activation of a signaling pathway by the physical translocation of a chromosome |
| title_short | Activation of a signaling pathway by the physical translocation of a chromosome |
| title_sort | activation of a signaling pathway by the physical translocation of a chromosome |
| url | https://hdl.handle.net/1721.1/144414 |
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