Probing Monotopic Phosphoglycosyl Transferases from Complex Cellular Milieu
Monotopic phosphoglycosyl transferase enzymes (monoPGTs) initiate the assembly of prokaryotic glycoconjugates essential for bacterial survival and proliferation. MonoPGTs belong to an expansive superfamily with a diverse and richly annotated sequence space; however, the biochemical roles of most mon...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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American Chemical Society (ACS)
2022
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| Online Access: | https://hdl.handle.net/1721.1/146813 |
| _version_ | 1826194737668816896 |
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| author | Anderson, Alyssa J Seebald, Leah M Arbour, Christine A Imperiali, Barbara |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Anderson, Alyssa J Seebald, Leah M Arbour, Christine A Imperiali, Barbara |
| author_sort | Anderson, Alyssa J |
| collection | MIT |
| description | Monotopic phosphoglycosyl transferase enzymes (monoPGTs) initiate the assembly of prokaryotic glycoconjugates essential for bacterial survival and proliferation. MonoPGTs belong to an expansive superfamily with a diverse and richly annotated sequence space; however, the biochemical roles of most monoPGTs in glycoconjugate biosynthesis pathways remain elusive. To better understand these critical enzymes, we have implemented activity-based protein profiling (ABPP) probes as protein-centric, membrane protein compatible tools that lay the groundwork for understanding the activity and regulation of the monoPGT superfamily from a cellular proteome. With straightforward gel-based readouts, we demonstrate robust, covalent labeling at the active site of various representative monoPGTs from cell membrane fractions using 3-phenyl-2H-azirine probes. |
| first_indexed | 2024-09-23T10:01:02Z |
| format | Article |
| id | mit-1721.1/146813 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T10:01:02Z |
| publishDate | 2022 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/1468132022-12-10T03:36:35Z Probing Monotopic Phosphoglycosyl Transferases from Complex Cellular Milieu Anderson, Alyssa J Seebald, Leah M Arbour, Christine A Imperiali, Barbara Massachusetts Institute of Technology. Department of Biology Monotopic phosphoglycosyl transferase enzymes (monoPGTs) initiate the assembly of prokaryotic glycoconjugates essential for bacterial survival and proliferation. MonoPGTs belong to an expansive superfamily with a diverse and richly annotated sequence space; however, the biochemical roles of most monoPGTs in glycoconjugate biosynthesis pathways remain elusive. To better understand these critical enzymes, we have implemented activity-based protein profiling (ABPP) probes as protein-centric, membrane protein compatible tools that lay the groundwork for understanding the activity and regulation of the monoPGT superfamily from a cellular proteome. With straightforward gel-based readouts, we demonstrate robust, covalent labeling at the active site of various representative monoPGTs from cell membrane fractions using 3-phenyl-2H-azirine probes. 2022-12-09T17:58:00Z 2022-12-09T17:58:00Z 2022-11-18 2022-12-09T17:47:04Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/146813 Anderson, Alyssa J, Seebald, Leah M, Arbour, Christine A and Imperiali, Barbara. 2022. "Probing Monotopic Phosphoglycosyl Transferases from Complex Cellular Milieu." ACS Chemical Biology, 17 (11). en 10.1021/acschembio.2c00648 ACS Chemical Biology Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf American Chemical Society (ACS) PMC |
| spellingShingle | Anderson, Alyssa J Seebald, Leah M Arbour, Christine A Imperiali, Barbara Probing Monotopic Phosphoglycosyl Transferases from Complex Cellular Milieu |
| title | Probing Monotopic Phosphoglycosyl Transferases from Complex Cellular Milieu |
| title_full | Probing Monotopic Phosphoglycosyl Transferases from Complex Cellular Milieu |
| title_fullStr | Probing Monotopic Phosphoglycosyl Transferases from Complex Cellular Milieu |
| title_full_unstemmed | Probing Monotopic Phosphoglycosyl Transferases from Complex Cellular Milieu |
| title_short | Probing Monotopic Phosphoglycosyl Transferases from Complex Cellular Milieu |
| title_sort | probing monotopic phosphoglycosyl transferases from complex cellular milieu |
| url | https://hdl.handle.net/1721.1/146813 |
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