Review: Advanced Atomic Force Microscopy Modes for Biomedical Research
Visualization of biomedical samples in their native environments at the microscopic scale is crucial for studying fundamental principles and discovering biomedical systems with complex interaction. The study of dynamic biological processes requires a microscope system with multiple modalities, high...
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| Format: | Article |
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Multidisciplinary Digital Publishing Institute
2022
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| Online Access: | https://hdl.handle.net/1721.1/146828 |
| _version_ | 1811089661317087232 |
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| author | Xia, Fangzhou Youcef-Toumi, Kamal |
| author2 | Massachusetts Institute of Technology. Mechatronics Research Laboratory |
| author_facet | Massachusetts Institute of Technology. Mechatronics Research Laboratory Xia, Fangzhou Youcef-Toumi, Kamal |
| author_sort | Xia, Fangzhou |
| collection | MIT |
| description | Visualization of biomedical samples in their native environments at the microscopic scale is crucial for studying fundamental principles and discovering biomedical systems with complex interaction. The study of dynamic biological processes requires a microscope system with multiple modalities, high spatial/temporal resolution, large imaging ranges, versatile imaging environments and ideally in-situ manipulation capabilities. Recent development of new Atomic Force Microscopy (AFM) capabilities has made it such a powerful tool for biological and biomedical research. This review introduces novel AFM functionalities including high-speed imaging for dynamic process visualization, mechanobiology with force spectroscopy, molecular species characterization, and AFM nano-manipulation. These capabilities enable many new possibilities for novel scientific research and allow scientists to observe and explore processes at the nanoscale like never before. Selected application examples from recent studies are provided to demonstrate the effectiveness of these AFM techniques. |
| first_indexed | 2024-09-23T14:22:44Z |
| format | Article |
| id | mit-1721.1/146828 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T14:22:44Z |
| publishDate | 2022 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | mit-1721.1/1468282023-07-05T20:03:02Z Review: Advanced Atomic Force Microscopy Modes for Biomedical Research Xia, Fangzhou Youcef-Toumi, Kamal Massachusetts Institute of Technology. Mechatronics Research Laboratory Visualization of biomedical samples in their native environments at the microscopic scale is crucial for studying fundamental principles and discovering biomedical systems with complex interaction. The study of dynamic biological processes requires a microscope system with multiple modalities, high spatial/temporal resolution, large imaging ranges, versatile imaging environments and ideally in-situ manipulation capabilities. Recent development of new Atomic Force Microscopy (AFM) capabilities has made it such a powerful tool for biological and biomedical research. This review introduces novel AFM functionalities including high-speed imaging for dynamic process visualization, mechanobiology with force spectroscopy, molecular species characterization, and AFM nano-manipulation. These capabilities enable many new possibilities for novel scientific research and allow scientists to observe and explore processes at the nanoscale like never before. Selected application examples from recent studies are provided to demonstrate the effectiveness of these AFM techniques. 2022-12-12T13:18:03Z 2022-12-12T13:18:03Z 2022-12-02 2022-12-09T20:23:08Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/146828 Biosensors 12 (12): 1116 (2022) PUBLISHER_CC http://dx.doi.org/10.3390/bios12121116 Creative Commons Attribution https://creativecommons.org/licenses/by/4.0/ application/pdf Multidisciplinary Digital Publishing Institute Multidisciplinary Digital Publishing Institute |
| spellingShingle | Xia, Fangzhou Youcef-Toumi, Kamal Review: Advanced Atomic Force Microscopy Modes for Biomedical Research |
| title | Review: Advanced Atomic Force Microscopy Modes for Biomedical Research |
| title_full | Review: Advanced Atomic Force Microscopy Modes for Biomedical Research |
| title_fullStr | Review: Advanced Atomic Force Microscopy Modes for Biomedical Research |
| title_full_unstemmed | Review: Advanced Atomic Force Microscopy Modes for Biomedical Research |
| title_short | Review: Advanced Atomic Force Microscopy Modes for Biomedical Research |
| title_sort | review advanced atomic force microscopy modes for biomedical research |
| url | https://hdl.handle.net/1721.1/146828 |
| work_keys_str_mv | AT xiafangzhou reviewadvancedatomicforcemicroscopymodesforbiomedicalresearch AT youceftoumikamal reviewadvancedatomicforcemicroscopymodesforbiomedicalresearch |