Electrochemical Ultrasensitive Sensing of Uric Acid on Non-Enzymatic Porous Cobalt Oxide Nanosheets-Based Sensor
Transition metal oxide (TMO)-based nanomaterials are effectively utilized to fabricate clinically useful ultra-sensitive sensors. Different nanostructured nanomaterials of TMO have attracted a lot of interest from researchers for diverse applications. Herein, we utilized a hydrothermal method to dev...
| Main Authors: | , , , , , , , |
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| Format: | Article |
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Multidisciplinary Digital Publishing Institute
2022
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| Online Access: | https://hdl.handle.net/1721.1/146832 |
| _version_ | 1811069056765132800 |
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| author | Masrat, Sakeena Nagal, Vandana Khan, Marya Moid, Iqra Alam, Shamshad Bhat, Kiesar Sideeq Khosla, Ajit Ahmad, Rafiq |
| author2 | Singapore-MIT Alliance in Research and Technology (SMART) |
| author_facet | Singapore-MIT Alliance in Research and Technology (SMART) Masrat, Sakeena Nagal, Vandana Khan, Marya Moid, Iqra Alam, Shamshad Bhat, Kiesar Sideeq Khosla, Ajit Ahmad, Rafiq |
| author_sort | Masrat, Sakeena |
| collection | MIT |
| description | Transition metal oxide (TMO)-based nanomaterials are effectively utilized to fabricate clinically useful ultra-sensitive sensors. Different nanostructured nanomaterials of TMO have attracted a lot of interest from researchers for diverse applications. Herein, we utilized a hydrothermal method to develop porous nanosheets of cobalt oxide. This synthesis method is simple and low temperature-based. The morphology of the porous nanosheets like cobalt oxide was investigated in detail using FESEM and TEM. The morphological investigation confirmed the successful formation of the porous nanosheet-like nanostructure. The crystal characteristic of porous cobalt oxide nanosheets was evaluated by XRD analysis, which confirmed the crystallinity of as-synthesized cobalt oxide nanosheets. The uric acid sensor fabrication involves the fixing of porous cobalt oxide nanosheets onto the GCE (glassy carbon electrode). The non-enzymatic electrochemical sensing was measured using CV and DPV analysis. The application of DPV technique during electrochemical testing for uric acid resulted in ultra-high sensitivity (3566.5 µAmM<sup>−1</sup>cm<sup>−2</sup>), which is ~7.58 times better than CV-based sensitivity (470.4 µAmM<sup>−1</sup>cm<sup>−2</sup>). Additionally, uric acid sensors were tested for their selectivity and storage ability. The applicability of the uric acid sensors was tested in the serum sample through standard addition and recovery of known uric acid concentration. This ultrasensitive nature of porous cobalt oxide nanosheets could be utilized to realize the sensing of other biomolecules. |
| first_indexed | 2024-09-23T08:05:00Z |
| format | Article |
| id | mit-1721.1/146832 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T08:05:00Z |
| publishDate | 2022 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | mit-1721.1/1468322023-07-05T20:28:55Z Electrochemical Ultrasensitive Sensing of Uric Acid on Non-Enzymatic Porous Cobalt Oxide Nanosheets-Based Sensor Masrat, Sakeena Nagal, Vandana Khan, Marya Moid, Iqra Alam, Shamshad Bhat, Kiesar Sideeq Khosla, Ajit Ahmad, Rafiq Singapore-MIT Alliance in Research and Technology (SMART) Transition metal oxide (TMO)-based nanomaterials are effectively utilized to fabricate clinically useful ultra-sensitive sensors. Different nanostructured nanomaterials of TMO have attracted a lot of interest from researchers for diverse applications. Herein, we utilized a hydrothermal method to develop porous nanosheets of cobalt oxide. This synthesis method is simple and low temperature-based. The morphology of the porous nanosheets like cobalt oxide was investigated in detail using FESEM and TEM. The morphological investigation confirmed the successful formation of the porous nanosheet-like nanostructure. The crystal characteristic of porous cobalt oxide nanosheets was evaluated by XRD analysis, which confirmed the crystallinity of as-synthesized cobalt oxide nanosheets. The uric acid sensor fabrication involves the fixing of porous cobalt oxide nanosheets onto the GCE (glassy carbon electrode). The non-enzymatic electrochemical sensing was measured using CV and DPV analysis. The application of DPV technique during electrochemical testing for uric acid resulted in ultra-high sensitivity (3566.5 µAmM<sup>−1</sup>cm<sup>−2</sup>), which is ~7.58 times better than CV-based sensitivity (470.4 µAmM<sup>−1</sup>cm<sup>−2</sup>). Additionally, uric acid sensors were tested for their selectivity and storage ability. The applicability of the uric acid sensors was tested in the serum sample through standard addition and recovery of known uric acid concentration. This ultrasensitive nature of porous cobalt oxide nanosheets could be utilized to realize the sensing of other biomolecules. 2022-12-12T13:30:56Z 2022-12-12T13:30:56Z 2022-12-07 2022-12-09T20:23:25Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/146832 Biosensors 12 (12): 1140 (2022) PUBLISHER_CC http://dx.doi.org/10.3390/bios12121140 Creative Commons Attribution https://creativecommons.org/licenses/by/4.0/ application/pdf Multidisciplinary Digital Publishing Institute Multidisciplinary Digital Publishing Institute |
| spellingShingle | Masrat, Sakeena Nagal, Vandana Khan, Marya Moid, Iqra Alam, Shamshad Bhat, Kiesar Sideeq Khosla, Ajit Ahmad, Rafiq Electrochemical Ultrasensitive Sensing of Uric Acid on Non-Enzymatic Porous Cobalt Oxide Nanosheets-Based Sensor |
| title | Electrochemical Ultrasensitive Sensing of Uric Acid on Non-Enzymatic Porous Cobalt Oxide Nanosheets-Based Sensor |
| title_full | Electrochemical Ultrasensitive Sensing of Uric Acid on Non-Enzymatic Porous Cobalt Oxide Nanosheets-Based Sensor |
| title_fullStr | Electrochemical Ultrasensitive Sensing of Uric Acid on Non-Enzymatic Porous Cobalt Oxide Nanosheets-Based Sensor |
| title_full_unstemmed | Electrochemical Ultrasensitive Sensing of Uric Acid on Non-Enzymatic Porous Cobalt Oxide Nanosheets-Based Sensor |
| title_short | Electrochemical Ultrasensitive Sensing of Uric Acid on Non-Enzymatic Porous Cobalt Oxide Nanosheets-Based Sensor |
| title_sort | electrochemical ultrasensitive sensing of uric acid on non enzymatic porous cobalt oxide nanosheets based sensor |
| url | https://hdl.handle.net/1721.1/146832 |
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