OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human
<jats:title>Abstract</jats:title><jats:p>Understanding the molecular underpinnings of pluripotency is a prerequisite for optimal maintenance and application of embryonic stem cells (ESCs). While the protein-protein interactions of core pluripotency factors have been identified in m...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer Science and Business Media LLC
2022
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| Online Access: | https://hdl.handle.net/1721.1/146844 |
| _version_ | 1811073511928627200 |
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| author | Huang, Xin Park, Kyoung-mi Gontarz, Paul Zhang, Bo Pan, Joshua McKenzie, Zachary Fischer, Laura A Dong, Chen Dietmann, Sabine Xing, Xiaoyun Shliaha, Pavel V Yang, Jihong Li, Dan Ding, Junjun Lungjangwa, Tenzin Mitalipova, Maya Khan, Shafqat A Imsoonthornruksa, Sumeth Jensen, Nick Wang, Ting Kadoch, Cigall Jaenisch, Rudolf Wang, Jianlong Theunissen, Thorold W |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Huang, Xin Park, Kyoung-mi Gontarz, Paul Zhang, Bo Pan, Joshua McKenzie, Zachary Fischer, Laura A Dong, Chen Dietmann, Sabine Xing, Xiaoyun Shliaha, Pavel V Yang, Jihong Li, Dan Ding, Junjun Lungjangwa, Tenzin Mitalipova, Maya Khan, Shafqat A Imsoonthornruksa, Sumeth Jensen, Nick Wang, Ting Kadoch, Cigall Jaenisch, Rudolf Wang, Jianlong Theunissen, Thorold W |
| author_sort | Huang, Xin |
| collection | MIT |
| description | <jats:title>Abstract</jats:title><jats:p>Understanding the molecular underpinnings of pluripotency is a prerequisite for optimal maintenance and application of embryonic stem cells (ESCs). While the protein-protein interactions of core pluripotency factors have been identified in mouse ESCs, their interactome in human ESCs (hESCs) has not to date been explored. Here we mapped the OCT4 interactomes in naïve and primed hESCs, revealing extensive connections to mammalian ATP-dependent nucleosome remodeling complexes. In naïve hESCs, OCT4 is associated with both BRG1 and BRM, the two paralog ATPases of the BAF complex. Genome-wide location analyses and genetic studies reveal that these two enzymes cooperate in a functionally redundant manner in the transcriptional regulation of blastocyst-specific genes. In contrast, in primed hESCs, OCT4 cooperates with BRG1 and SOX2 to promote chromatin accessibility at ectodermal genes. This work reveals how a common transcription factor utilizes differential BAF complexes to control distinct transcriptional programs in naïve and primed hESCs.</jats:p> |
| first_indexed | 2024-09-23T09:34:09Z |
| format | Article |
| id | mit-1721.1/146844 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T09:34:09Z |
| publishDate | 2022 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1468442022-12-13T03:40:44Z OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human Huang, Xin Park, Kyoung-mi Gontarz, Paul Zhang, Bo Pan, Joshua McKenzie, Zachary Fischer, Laura A Dong, Chen Dietmann, Sabine Xing, Xiaoyun Shliaha, Pavel V Yang, Jihong Li, Dan Ding, Junjun Lungjangwa, Tenzin Mitalipova, Maya Khan, Shafqat A Imsoonthornruksa, Sumeth Jensen, Nick Wang, Ting Kadoch, Cigall Jaenisch, Rudolf Wang, Jianlong Theunissen, Thorold W Massachusetts Institute of Technology. Department of Biology <jats:title>Abstract</jats:title><jats:p>Understanding the molecular underpinnings of pluripotency is a prerequisite for optimal maintenance and application of embryonic stem cells (ESCs). While the protein-protein interactions of core pluripotency factors have been identified in mouse ESCs, their interactome in human ESCs (hESCs) has not to date been explored. Here we mapped the OCT4 interactomes in naïve and primed hESCs, revealing extensive connections to mammalian ATP-dependent nucleosome remodeling complexes. In naïve hESCs, OCT4 is associated with both BRG1 and BRM, the two paralog ATPases of the BAF complex. Genome-wide location analyses and genetic studies reveal that these two enzymes cooperate in a functionally redundant manner in the transcriptional regulation of blastocyst-specific genes. In contrast, in primed hESCs, OCT4 cooperates with BRG1 and SOX2 to promote chromatin accessibility at ectodermal genes. This work reveals how a common transcription factor utilizes differential BAF complexes to control distinct transcriptional programs in naïve and primed hESCs.</jats:p> 2022-12-12T15:40:24Z 2022-12-12T15:40:24Z 2021 2022-12-12T15:36:35Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/146844 Huang, Xin, Park, Kyoung-mi, Gontarz, Paul, Zhang, Bo, Pan, Joshua et al. 2021. "OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human." Nature Communications, 12 (1). en 10.1038/S41467-021-25107-3 Nature Communications Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Springer Science and Business Media LLC Nature |
| spellingShingle | Huang, Xin Park, Kyoung-mi Gontarz, Paul Zhang, Bo Pan, Joshua McKenzie, Zachary Fischer, Laura A Dong, Chen Dietmann, Sabine Xing, Xiaoyun Shliaha, Pavel V Yang, Jihong Li, Dan Ding, Junjun Lungjangwa, Tenzin Mitalipova, Maya Khan, Shafqat A Imsoonthornruksa, Sumeth Jensen, Nick Wang, Ting Kadoch, Cigall Jaenisch, Rudolf Wang, Jianlong Theunissen, Thorold W OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human |
| title | OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human |
| title_full | OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human |
| title_fullStr | OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human |
| title_full_unstemmed | OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human |
| title_short | OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human |
| title_sort | oct4 cooperates with distinct atp dependent chromatin remodelers in naive and primed pluripotent states in human |
| url | https://hdl.handle.net/1721.1/146844 |
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