Air-liquid interface culture promotes maturation and allows environmental exposure of pluripotent stem cell–derived alveolar epithelium
Type 2 alveolar epithelial cells (AT2s), facultative progenitor cells of the lung alveolus, play a vital role in the biology of the distal lung. In vitro model systems that incorporate human cells, recapitulate the biology of primary AT2s, and interface with the outside environment could serve as us...
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
American Society for Clinical Investigation
2022
|
| Online Access: | https://hdl.handle.net/1721.1/146847 |
| _version_ | 1826193421590593536 |
|---|---|
| author | Abo, Kristine M Sainz de Aja, Julio Lindstrom-Vautrin, Jonathan Alysandratos, Konstantinos-Dionysios Richards, Alexsia Garcia-de-Alba, Carolina Huang, Jessie Hix, Olivia T Werder, Rhiannon B Bullitt, Esther Hinds, Anne Falconer, Isaac Villacorta-Martin, Carlos Jaenisch, Rudolf Kim, Carla F Kotton, Darrell N Wilson, Andrew A |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Abo, Kristine M Sainz de Aja, Julio Lindstrom-Vautrin, Jonathan Alysandratos, Konstantinos-Dionysios Richards, Alexsia Garcia-de-Alba, Carolina Huang, Jessie Hix, Olivia T Werder, Rhiannon B Bullitt, Esther Hinds, Anne Falconer, Isaac Villacorta-Martin, Carlos Jaenisch, Rudolf Kim, Carla F Kotton, Darrell N Wilson, Andrew A |
| author_sort | Abo, Kristine M |
| collection | MIT |
| description | Type 2 alveolar epithelial cells (AT2s), facultative progenitor cells of the lung alveolus, play a vital role in the biology of the distal lung. In vitro model systems that incorporate human cells, recapitulate the biology of primary AT2s, and interface with the outside environment could serve as useful tools to elucidate functional characteristics of AT2s in homeostasis and disease. We and others recently adapted human induced pluripotent stem cell-derived AT2s (iAT2s) for air-liquid interface (ALI) culture. Here, we comprehensively characterize the effects of ALI culture on iAT2s and benchmark their transcriptional profile relative to both freshly sorted and cultured primary human fetal and adult AT2s. We find that iAT2s cultured at ALI maintain an AT2 phenotype while upregulating expression of transcripts associated with AT2 maturation. We then leverage this platform to assay the effects of exposure to clinically significant, inhaled toxicants including cigarette smoke and electronic cigarette vapor. |
| first_indexed | 2024-09-23T09:39:00Z |
| format | Article |
| id | mit-1721.1/146847 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T09:39:00Z |
| publishDate | 2022 |
| publisher | American Society for Clinical Investigation |
| record_format | dspace |
| spelling | mit-1721.1/1468472022-12-13T03:40:06Z Air-liquid interface culture promotes maturation and allows environmental exposure of pluripotent stem cell–derived alveolar epithelium Abo, Kristine M Sainz de Aja, Julio Lindstrom-Vautrin, Jonathan Alysandratos, Konstantinos-Dionysios Richards, Alexsia Garcia-de-Alba, Carolina Huang, Jessie Hix, Olivia T Werder, Rhiannon B Bullitt, Esther Hinds, Anne Falconer, Isaac Villacorta-Martin, Carlos Jaenisch, Rudolf Kim, Carla F Kotton, Darrell N Wilson, Andrew A Massachusetts Institute of Technology. Department of Biology Type 2 alveolar epithelial cells (AT2s), facultative progenitor cells of the lung alveolus, play a vital role in the biology of the distal lung. In vitro model systems that incorporate human cells, recapitulate the biology of primary AT2s, and interface with the outside environment could serve as useful tools to elucidate functional characteristics of AT2s in homeostasis and disease. We and others recently adapted human induced pluripotent stem cell-derived AT2s (iAT2s) for air-liquid interface (ALI) culture. Here, we comprehensively characterize the effects of ALI culture on iAT2s and benchmark their transcriptional profile relative to both freshly sorted and cultured primary human fetal and adult AT2s. We find that iAT2s cultured at ALI maintain an AT2 phenotype while upregulating expression of transcripts associated with AT2 maturation. We then leverage this platform to assay the effects of exposure to clinically significant, inhaled toxicants including cigarette smoke and electronic cigarette vapor. 2022-12-12T18:36:42Z 2022-12-12T18:36:42Z 2022 2022-12-12T18:27:25Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/146847 Abo, Kristine M, Sainz de Aja, Julio, Lindstrom-Vautrin, Jonathan, Alysandratos, Konstantinos-Dionysios, Richards, Alexsia et al. 2022. "Air-liquid interface culture promotes maturation and allows environmental exposure of pluripotent stem cell–derived alveolar epithelium." JCI Insight, 7 (6). en 10.1172/JCI.INSIGHT.155589 JCI Insight Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf American Society for Clinical Investigation American Society for Clinical Investigation |
| spellingShingle | Abo, Kristine M Sainz de Aja, Julio Lindstrom-Vautrin, Jonathan Alysandratos, Konstantinos-Dionysios Richards, Alexsia Garcia-de-Alba, Carolina Huang, Jessie Hix, Olivia T Werder, Rhiannon B Bullitt, Esther Hinds, Anne Falconer, Isaac Villacorta-Martin, Carlos Jaenisch, Rudolf Kim, Carla F Kotton, Darrell N Wilson, Andrew A Air-liquid interface culture promotes maturation and allows environmental exposure of pluripotent stem cell–derived alveolar epithelium |
| title | Air-liquid interface culture promotes maturation and allows environmental exposure of pluripotent stem cell–derived alveolar epithelium |
| title_full | Air-liquid interface culture promotes maturation and allows environmental exposure of pluripotent stem cell–derived alveolar epithelium |
| title_fullStr | Air-liquid interface culture promotes maturation and allows environmental exposure of pluripotent stem cell–derived alveolar epithelium |
| title_full_unstemmed | Air-liquid interface culture promotes maturation and allows environmental exposure of pluripotent stem cell–derived alveolar epithelium |
| title_short | Air-liquid interface culture promotes maturation and allows environmental exposure of pluripotent stem cell–derived alveolar epithelium |
| title_sort | air liquid interface culture promotes maturation and allows environmental exposure of pluripotent stem cell derived alveolar epithelium |
| url | https://hdl.handle.net/1721.1/146847 |
| work_keys_str_mv | AT abokristinem airliquidinterfaceculturepromotesmaturationandallowsenvironmentalexposureofpluripotentstemcellderivedalveolarepithelium AT sainzdeajajulio airliquidinterfaceculturepromotesmaturationandallowsenvironmentalexposureofpluripotentstemcellderivedalveolarepithelium AT lindstromvautrinjonathan airliquidinterfaceculturepromotesmaturationandallowsenvironmentalexposureofpluripotentstemcellderivedalveolarepithelium AT alysandratoskonstantinosdionysios airliquidinterfaceculturepromotesmaturationandallowsenvironmentalexposureofpluripotentstemcellderivedalveolarepithelium AT richardsalexsia airliquidinterfaceculturepromotesmaturationandallowsenvironmentalexposureofpluripotentstemcellderivedalveolarepithelium AT garciadealbacarolina airliquidinterfaceculturepromotesmaturationandallowsenvironmentalexposureofpluripotentstemcellderivedalveolarepithelium AT huangjessie airliquidinterfaceculturepromotesmaturationandallowsenvironmentalexposureofpluripotentstemcellderivedalveolarepithelium AT hixoliviat airliquidinterfaceculturepromotesmaturationandallowsenvironmentalexposureofpluripotentstemcellderivedalveolarepithelium AT werderrhiannonb airliquidinterfaceculturepromotesmaturationandallowsenvironmentalexposureofpluripotentstemcellderivedalveolarepithelium AT bullittesther airliquidinterfaceculturepromotesmaturationandallowsenvironmentalexposureofpluripotentstemcellderivedalveolarepithelium AT hindsanne airliquidinterfaceculturepromotesmaturationandallowsenvironmentalexposureofpluripotentstemcellderivedalveolarepithelium AT falconerisaac airliquidinterfaceculturepromotesmaturationandallowsenvironmentalexposureofpluripotentstemcellderivedalveolarepithelium AT villacortamartincarlos airliquidinterfaceculturepromotesmaturationandallowsenvironmentalexposureofpluripotentstemcellderivedalveolarepithelium AT jaenischrudolf airliquidinterfaceculturepromotesmaturationandallowsenvironmentalexposureofpluripotentstemcellderivedalveolarepithelium AT kimcarlaf airliquidinterfaceculturepromotesmaturationandallowsenvironmentalexposureofpluripotentstemcellderivedalveolarepithelium AT kottondarrelln airliquidinterfaceculturepromotesmaturationandallowsenvironmentalexposureofpluripotentstemcellderivedalveolarepithelium AT wilsonandrewa airliquidinterfaceculturepromotesmaturationandallowsenvironmentalexposureofpluripotentstemcellderivedalveolarepithelium |