Toxoplasma gondii exploits the host ESCRT machinery for parasite uptake of host cytosolic proteins
<jats:p><jats:italic>Toxoplasma gondii</jats:italic> is a master manipulator capable of effectively siphoning the resources from the host cell for its intracellular subsistence. However, the molecular underpinnings of how the parasite gains resources from its host remain largely un...
| Main Authors: | , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022
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| Online Access: | https://hdl.handle.net/1721.1/146900 |
| _version_ | 1811077286461440000 |
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| author | Rivera-Cuevas, Yolanda Mayoral, Joshua Di Cristina, Manlio Lawrence, Anna-Lisa E Olafsson, Einar B Patel, Romir K Thornhill, Dishari Waldman, Benjamin S Ono, Akira Sexton, Jonathan Z Lourido, Sebastian Weiss, Louis M Carruthers, Vern B |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Rivera-Cuevas, Yolanda Mayoral, Joshua Di Cristina, Manlio Lawrence, Anna-Lisa E Olafsson, Einar B Patel, Romir K Thornhill, Dishari Waldman, Benjamin S Ono, Akira Sexton, Jonathan Z Lourido, Sebastian Weiss, Louis M Carruthers, Vern B |
| author_sort | Rivera-Cuevas, Yolanda |
| collection | MIT |
| description | <jats:p><jats:italic>Toxoplasma gondii</jats:italic> is a master manipulator capable of effectively siphoning the resources from the host cell for its intracellular subsistence. However, the molecular underpinnings of how the parasite gains resources from its host remain largely unknown. Residing within a non-fusogenic parasitophorous vacuole (PV), the parasite must acquire resources across the limiting membrane of its replicative niche, which is decorated with parasite proteins including those secreted from dense granules. We discovered a role for the host Endosomal Sorting Complex Required for Transport (ESCRT) machinery in host cytosolic protein uptake by <jats:italic>T</jats:italic>. <jats:italic>gondii</jats:italic> by disrupting host ESCRT function. We identified the transmembrane dense granule protein TgGRA14, which contains motifs homologous to the late domain motifs of HIV-1 Gag, as a candidate for the recruitment of the host ESCRT machinery to the PV membrane. Using an HIV-1 virus-like particle (VLP) release assay, we found that the motif-containing portion of TgGRA14 is sufficient to substitute for HIV-1 Gag late domain to mediate ESCRT-dependent VLP budding. We also show that TgGRA14 is proximal to and interacts with host ESCRT components and other dense granule proteins during infection. Furthermore, analysis of TgGRA14-deficient parasites revealed a marked reduction in ingestion of a host cytosolic protein compared to WT parasites. Thus, we propose a model in which <jats:italic>T</jats:italic>. <jats:italic>gondii</jats:italic> recruits the host ESCRT machinery to the PV where it can interact with TgGRA14 for the internalization of host cytosolic proteins across the PV membrane (PVM). These findings provide new insight into how <jats:italic>T</jats:italic>. <jats:italic>gondii</jats:italic> accesses contents of the host cytosol by exploiting a key pathway for vesicular budding and membrane scission.</jats:p> |
| first_indexed | 2024-09-23T10:40:32Z |
| format | Article |
| id | mit-1721.1/146900 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T10:40:32Z |
| publishDate | 2022 |
| publisher | Public Library of Science (PLoS) |
| record_format | dspace |
| spelling | mit-1721.1/1469002022-12-17T03:34:28Z Toxoplasma gondii exploits the host ESCRT machinery for parasite uptake of host cytosolic proteins Rivera-Cuevas, Yolanda Mayoral, Joshua Di Cristina, Manlio Lawrence, Anna-Lisa E Olafsson, Einar B Patel, Romir K Thornhill, Dishari Waldman, Benjamin S Ono, Akira Sexton, Jonathan Z Lourido, Sebastian Weiss, Louis M Carruthers, Vern B Massachusetts Institute of Technology. Department of Biology <jats:p><jats:italic>Toxoplasma gondii</jats:italic> is a master manipulator capable of effectively siphoning the resources from the host cell for its intracellular subsistence. However, the molecular underpinnings of how the parasite gains resources from its host remain largely unknown. Residing within a non-fusogenic parasitophorous vacuole (PV), the parasite must acquire resources across the limiting membrane of its replicative niche, which is decorated with parasite proteins including those secreted from dense granules. We discovered a role for the host Endosomal Sorting Complex Required for Transport (ESCRT) machinery in host cytosolic protein uptake by <jats:italic>T</jats:italic>. <jats:italic>gondii</jats:italic> by disrupting host ESCRT function. We identified the transmembrane dense granule protein TgGRA14, which contains motifs homologous to the late domain motifs of HIV-1 Gag, as a candidate for the recruitment of the host ESCRT machinery to the PV membrane. Using an HIV-1 virus-like particle (VLP) release assay, we found that the motif-containing portion of TgGRA14 is sufficient to substitute for HIV-1 Gag late domain to mediate ESCRT-dependent VLP budding. We also show that TgGRA14 is proximal to and interacts with host ESCRT components and other dense granule proteins during infection. Furthermore, analysis of TgGRA14-deficient parasites revealed a marked reduction in ingestion of a host cytosolic protein compared to WT parasites. Thus, we propose a model in which <jats:italic>T</jats:italic>. <jats:italic>gondii</jats:italic> recruits the host ESCRT machinery to the PV where it can interact with TgGRA14 for the internalization of host cytosolic proteins across the PV membrane (PVM). These findings provide new insight into how <jats:italic>T</jats:italic>. <jats:italic>gondii</jats:italic> accesses contents of the host cytosol by exploiting a key pathway for vesicular budding and membrane scission.</jats:p> 2022-12-16T17:40:02Z 2022-12-16T17:40:02Z 2021 2022-12-16T17:36:15Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/146900 Rivera-Cuevas, Yolanda, Mayoral, Joshua, Di Cristina, Manlio, Lawrence, Anna-Lisa E, Olafsson, Einar B et al. 2021. "Toxoplasma gondii exploits the host ESCRT machinery for parasite uptake of host cytosolic proteins." PLoS Pathogens, 17 (12). en 10.1371/JOURNAL.PPAT.1010138 PLoS Pathogens Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Public Library of Science (PLoS) PLoS |
| spellingShingle | Rivera-Cuevas, Yolanda Mayoral, Joshua Di Cristina, Manlio Lawrence, Anna-Lisa E Olafsson, Einar B Patel, Romir K Thornhill, Dishari Waldman, Benjamin S Ono, Akira Sexton, Jonathan Z Lourido, Sebastian Weiss, Louis M Carruthers, Vern B Toxoplasma gondii exploits the host ESCRT machinery for parasite uptake of host cytosolic proteins |
| title | Toxoplasma gondii exploits the host ESCRT machinery for parasite uptake of host cytosolic proteins |
| title_full | Toxoplasma gondii exploits the host ESCRT machinery for parasite uptake of host cytosolic proteins |
| title_fullStr | Toxoplasma gondii exploits the host ESCRT machinery for parasite uptake of host cytosolic proteins |
| title_full_unstemmed | Toxoplasma gondii exploits the host ESCRT machinery for parasite uptake of host cytosolic proteins |
| title_short | Toxoplasma gondii exploits the host ESCRT machinery for parasite uptake of host cytosolic proteins |
| title_sort | toxoplasma gondii exploits the host escrt machinery for parasite uptake of host cytosolic proteins |
| url | https://hdl.handle.net/1721.1/146900 |
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